Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro

Matand, Kanyand; Dessai, Ananta Porobo; Prakash, Channapatna S.

doi:10.1007/bf00233288

Cited by 47 publications

(27 citation statements)

References 14 publications

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“…The stimulatory effect of TDZ on multiple shoot formation has been reported earlier by Huetteman and Preece (1993) in woody trees, Kanyand et al (1994) in Arachis hypogea, Ahmad and Anis (2007) in Cyamopsis tetragonoloba and Siddique and Anis (2007) in Cassia angustifolia. Similarly, Ismail et al (2011) successfully regenerated multiple shoots in nodal segments of C. ternatea using TDZ.…”

Section: Effect Of Thidiazuronsupporting

confidence: 67%

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RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05-2.5 μM) was used as a supplement to the Murashige and Skoog's (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.

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Section: Effect Of Thidiazuronsupporting

confidence: 67%

“…TDZ has been successfully used in plant regeneration systems for many plant species (Faisal et al 2005;Ahmad et al 2006;Ahmad and Anis 2007a) including various legumes (Malik and Saxena 1992;Kanyand et al 1994;Sanago et al 1996;Eapen et al 1998;Victor et al 1999;Ahmad and Anis 2007b;Barik et al 2007). …”

Section: Effect Of Thidiazuronmentioning

confidence: 99%

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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“…This is similar to the results observed in peanut (Kanyand et al, 1994). In a subsequent experiment, a regeneration medium containing 1 mg l Ϫ1 of TDZ was compared with media containing 1 mg l Ϫ1 2,4-D and 1 mg l Ϫ1 BAP (Jähne et al, 1991) or 1 mg l Ϫ1 IAA and 0.2 mg l Ϫ1 kinetin (Wan and Lemaux, 1994) which are often used for barley regeneration.…”

Section: Resultssupporting

confidence: 76%

Thidiazuron promotes in vitro regeneration of wheat and barley

Shan

2000

In Vitro Cell.Dev.Biol.-Plant

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SummaryThidiazuron (TDZ) is a substituted phenylurea which has been shown to be an efficacious regulator of in vitro morphogenesis of many dicot plant species. However, information regarding the effect of TDZ on in vitro regeneration of monocot species is limited. We investigated the effects of TDZ on in vitro regeneration of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) and found that it promoted shoot regeneration from callus in these two important cereal species. Plant regeneration from calluses derived from immature embryo culture of barley and wheat was observed in regeneration media with a wide range of TDZ concentrations (0.045-45 mM). Shoot regeneration from barley calluses was the highest (38.3% for cv. Golden Promise) at 4.5 mM (1 mg l Ϫ1) TDZ, while the optimal TDZ level for wheat regeneration seemed to be 0.9 mM (0.2 mg l Ϫ1 ) (87% for cv. Bob White and 49.4% for cv. Hi-Line). Roots developed normally when the regenerated wheat and barley shoots from TDZ-containing media were transferred to the rooting medium. Comparison with other plant growth regulators commonly used in wheat and barley regeneration media suggested that TDZ was among the best for in vitro regeneration of wheat and barley.

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“…Similar results were obtained with TDZ compared to 2,4-D, KIN, and NAA in Sugarcane (Saccharum spp.) (Gallo-Meagher et al 2000), beans (Phaseolus vulgaris) (Malik and Saxena 1992a), chickpea (Cicer arietinum) (George and Eapen 1997), and peanut (Arachis hypogaea) (Kanyand et al 1994). In pigeon pea, higher regeneration of shoot buds (14.7) from the embryonic axes explants was observed on 0.5 mg L −1 TDZ.…”

Section: Shoot Bud Regenerationmentioning

confidence: 99%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro

Cited by 47 publications

References 14 publications

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Thidiazuron promotes in vitro regeneration of wheat and barley

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

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