Thidiazuron promotes in vitro regeneration of wheat and barley

Shan, Xueyan; Li, Desen; Qu, Rongda

doi:10.1007/s11627-000-0038-y

Cited by 53 publications

(34 citation statements)

References 20 publications

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“…As citocininas promovem a proliferação celular dos meristemas laterais, quebram a dormência de gemas em videira e macieira e facilitam a proliferação de múltiplos brotos em algumas culturas (GAIRI e RASHID, 2004;SRIVATANAKUL et al, 2000), incluindo o arroz (YOOKONGKAEW et al, 2007) e outras espécies como feijão (MOHAMED et al, 2006) e trigo (SHAN et al, 2000). Liu et al, (2011) indicam que o desenvolvimento de gemas que originam perfilhos nas plantas de arroz é estimulado pela citocinina, de modo que o aumento no nível desses hormônios favorece o perfilhamento.…”

Section: Resultsunclassified

INFLUÊNCIA DO THIDIAZURON E DA INOCULAÇÃO COM Azospirillum brasilense NO CRESCIMENTO E PRODUTIVIDADE DO ARROZ DE TERRAS ALTAS

Garé

Buzo²,

Arf³

et al. 2017

Revista Brasileira De Engenharia De Biossistemas

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A inoculação com bactérias do gênero Azospirillum pode contribuir com o fornecimento de nitrogênio (N) e com o crescimento das plantas, pois esses microrganismos são também promotores de crescimento. Por outro lado a aplicação nas doses e épocas adequadas do regulador vegetal de efeito citocinínico thidiazuron (TDZ) pode vir a beneficiar a produtividade de grãos. Dessa maneira, o objetivo do trabalho foi avaliar o efeito de doses de TDZ sobre a produtividade do arroz, na presença e ausência da inoculação foliar com Azospirillum brasilense. Foi utilizada a cultivar ANa 5015, no delineamento experimental de blocos casualizados em esquema fatorial 4 x 2, sendo quatro doses de TDZ (0,0; 0,5; 1,0 e 1,5g ha -1 ) aplicadas por ocasião do perfilhamento, 30 DAE, e da aplicação de Azospirillum brasilense (presença ou ausência) em dose fixa aos 20 DAE. Verificou-se que a cultivar ANa 5015 não se mostrou responsiva para a inoculação foliar com Azospirillum brasilense na dose de 200 mL ha -1 . Para as doses de TDZ aplicadas via foliar, houve uma variação significativa na altura de plantas, cuja dose de 0,41 g ha -1 resultou na altura máxima de 1,11 m. Não houve efeito das doses de TDZ na produtividade, massa de 100 grãos, número de panículas por m², grãos por panícula e massa hectolítrica. Palavras-chave: bactérias diazotróficas, irrigação por aspersão, Oryza sativa L., thidiazuron INFLUENCE OF THIDIAZURON AND INOCULATION WITH Azospirillum brasilense IN THE GROWTH AND PRODUCTIVITY OF HIGHLAND RICE ABSTRACTInoculation with bacteria of Azospirillum genus may contribute with the nitrogen (N) supply and the plant growth, since these microorganisms are also growth promoters. On the other hand the application in the appropriate doses and times of the plant regulator of cytokinin effect thidiazuron (TDZ) may benefit grain yield. Thus, the goal of this work was to evaluate the TDZ dose effect on rice yield, in the presence and absence of leaf inoculation with Azospirillum brasilense. The cultivar ANa 5015 was used in the experimental design of randomized blocks in a 4 x 2 factorial scheme, four TDZ (0,0; 0,5; 1,0 and 1,5g ha -1 ) doses applied on the occasion of tillering , 30 DAE, and the application of Azospirillum brasilense (presence or absence) at a fixed dose at 20 DAE. It was verified that the cultivar ANa 5015 was not responsive to the leaf inoculation with Azospirillum brasilense at the dose of 200 mL ha -1 . For TDZ doses applied via leaf, there was a significant variation in plant height, whose *

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Section: Resultsunclassified

INFLUÊNCIA DO THIDIAZURON E DA INOCULAÇÃO COM Azospirillum brasilense NO CRESCIMENTO E PRODUTIVIDADE DO ARROZ DE TERRAS ALTAS

Garé

Buzo²,

Arf³

et al. 2017

Revista Brasileira De Engenharia De Biossistemas

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“…TDZ (Thidiazuron) is a substituted phenyl urea [1-phenyl-3-(1,2,3-thidiazol-5-yl)urea] that has immense potential as a cytokinin in shoot organogenesis in a large number of plant systems including legumes (Murthy et al, 1998;Shan et al, 2000;Ganeshan et al, 2003;Onamu et al, 2003;Mroginski et al, 2004;Sharma et al, 2005;Chen and Chang, 2006;Radhika et al, 2006). Since, the observation made by Malik and Saxena (1992) TDZ has been noted to be the most promising cytokinin for shoot induction in chickpea by number of research teams (Barna and Wakhlu, 1993;Huetteman and Preece, 1993;Murthy et al, 1996;Rizvi and Singh, 2000;Jayanand et al, 2003;Senthil et al, 2004;TewariSingh et al, 2004;Kiran et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

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A rapid, reproducible and efficient regeneration method was developed for chickpea (Cicer arietinum L.) using single cotyledon with half embryonal axis as explants. MS medium supplemented with 4 ìM TDZ, 10 ìM 2-iP and 2 ìM kinetin induced 50-100 adventitious buds/shoots after 14 days of culture and elongated on MS medium supplemented with 5 ìM 2-iP and 2 ìM kinetin. Healthy, strong and 100 % rooting was achieved by exposing cut ends of the shoots to 10 sec pulse treatment with 100 ìmoles/ml IBA followed by their transfer to liquid MS basal medium within 10-14 d. 2-3 cm long shoots were most suitable for rooting. Potting-mixture with good aeration and lesser capacity to retain water was most suitable for achieving successful establishment of chickpea plantlets. Garden soil mixed with sand (gravel) and bio-manure in the ratio of 1:1:1 is most suitable for achieving cent percent transplantation success. Cent percent of plantlets got acclimatized, survived in the pots and showed normal growth, development, flowering followed by podding and seeds setting. Harvesting of seeds was done after the pods were fully matured and dry. In this communication, we have demonstrated for the first time that shoot length, pulse treatment of cut ends of shoots with 100 ìmoles/ml IBA and aeration of potting mixture are key factors for rapid micro-propagation and successful establishment of chickpea.

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“…This showed that the low concentration of IBA in combination with BAP or TDZ was needed for shoot regeneration of sorghum. Shan et al (2000) and Aparna and Rashid (2004) demonstrate that TDZ is an important hormone for the regeneration of cereal and grass species and the production of multiple shoots. Sorghum regeneration is affected by genotype (Baskaran and Jayabalan 2005), which may be due to changes in gene expression, which in turn depends on the spatial and temporal repartition of physiological stages (Vasil and Vasil 1986).…”

Section: Discussionmentioning

confidence: 99%

<i>In Vitro</i> Induction of Polyploidy in <i>Sorghum bicolor</i> L.

2015

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SummaryIn this study the effect of different colchicine concentrations (0.025, 0.05, 0.1 and 0.2%) and treatment time (8, 24 and 48 h) on in vitro polyploidy induction from shoot tips was investigated as a factorial experiment based on completely randomized design with three replications. Polyploidy induction was confirmed by chromosome counts, size and number of stomata and other morphological characters. By assessment of different media, it was revealed that the suitable medium for regeneration of shoot tips is MS medium supplemented with 0.1 mg L 1 IBA (indole-3-butyric acid) and 1 or 2 mg L 1 BAP (6-benzylaminopurine) or 2 mg L 1 TDZ (thidiazuron). In addition, MS medium supplemented with 1 mg L 1 NAA (1-naphthaleneacetic acid) and 0.5 or 1 mg L 1 IBA led to the maximum rooting of plantlets. With increasing colchicine concentration and its treatment duration, explants survival and their rooting considerably decreased. The results revealed that 0.1% concentration of colchicine with 48 h of treatment established the maximum amount of the in vitro induced tetraploid plantlets. The derived tetraploid plantlets had bigger stomata with lower density. Chlorophyll content in tetraploid plantlets was significantly higher than diploids, and they also showed an elevated level of antioxidant enzymes, protein and soluble carbohydrate contents as compared with diploids.

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Thidiazuron promotes in vitro regeneration of wheat and barley

Cited by 53 publications

References 20 publications

INFLUÊNCIA DO THIDIAZURON E DA INOCULAÇÃO COM Azospirillum brasilense NO CRESCIMENTO E PRODUTIVIDADE DO ARROZ DE TERRAS ALTAS

INFLUÊNCIA DO THIDIAZURON E DA INOCULAÇÃO COM Azospirillum brasilense NO CRESCIMENTO E PRODUTIVIDADE DO ARROZ DE TERRAS ALTAS

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

<i>In Vitro</i> Induction of Polyploidy in <i>Sorghum bicolor</i> L.

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