Thin‐Chip Microspray System for High‐Performance Fourier‐Transform Ion‐Cyclotron Resonance Mass Spectrometry of Biopolymers

Rossier, Joël S.; Youhnovski, Nikolay; Lion, Niels; Damoc, Eugen; Becker, Susanne; Reymond, Frédéric; Girault, Hubert H.; Przybylski, Michael

doi:10.1002/anie.200390050

Cited by 60 publications

(56 citation statements)

References 33 publications

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“…These devices are now being microfabricated with technologies imported from the microelectronics industry to improve reproducibility and robustness of the system. Many technological approaches have been based on polymer [10][11][12][13] and glass or silicon [14,15] microfabrication, among them are mature devices working either with robotic stations such as the Nanomate TM system from Advion Biosciences [16 ] or stand-alone disposable devices [17]. This kind of micro-or nanospray microfabricated emitter is appearing at a time when exciting developments in mass spectrometry are starting to emerge, such as new methodologies based on the capabilities of MS for the structural elucidation of protein complexes or complex mixture analysis.…”

Section: Sample Volume Reductionmentioning

confidence: 99%

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Why the move to microfluidics for protein analysis?

Lion

Reymond

Girault

et al. 2004

Current Opinion in Biotechnology

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106

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There has been a recent trend towards the miniaturization of analytical tools, but what are the advantages of microfluidic devices and when is their use appropriate? Recent advances in the field of micro-analytical systems can be classified according to instrument performance (which refers here to the desired property of the analytical tool of interest) and two important features specifically related to miniaturisation, namely reduction of the sample volume and the time-to-result. Here we discuss the contribution of these different parameters and aim to highlight the factors of choice in the development and use of microfluidic devices dedicated to protein analysis.

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Section: Sample Volume Reductionmentioning

confidence: 99%

“…For this kind of methodology, microchips (e.g. thin polymer infusion microtips [17]) seem a particularly valuable option, as they can combine small sample volumes with high-performance results and flexible experimental times.…”

Section: Sample Volume Reductionmentioning

confidence: 99%

Why the move to microfluidics for protein analysis?

Lion

Reymond

Girault

et al. 2004

Current Opinion in Biotechnology

Self Cite

106

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“…"Needle-like" approaches were reported using silicon nitride [11], parylene [12], silicon [13][14][15], as well as polycarbonate [16] or polymethyl methacrylate (PMMA) [17]. The reported "channel-like" approaches include PMMA starshaped systems [18], polydimethyl siloxane (PDMS) devices having a triangular shape [19], devices based on a groove feature [20], machined point-like structures [21], polyimide-based triangular systems [22], and triangular parylene sheet-based devices [23].…”

mentioning

confidence: 99%

An open design microfabricated nib-like nanoelectrospray emitter tip on a conducting silicon substrate for the application of the ionization voltage

Gac

Rolando

Arscott

2006

J. Am. Soc. Mass Spectrom.

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This paper describes a novel emitter tip having the shape of a nib and based on an open structure for nano-electrospray ionization mass spectrometry (nanoESI-MS). The nib structure is fabricated with standard lithography techniques using SU-8, an epoxy-based negative photoresist. The tip is comprised of a reservoir, a capillary slot and a point-like feature, and is fabricated on a silicon wafer. We present here a novel scheme for interfacing such nib tips to MS by applying the ionization voltage directly onto the semi-conductor support. The silicon support is in direct contact with the liquid to be analyzed at the reservoir and microchannel level, thus allowing easy use in ESI-MS. This scheme is especially advantageous for automated analysis as the manual step of positioning a metallic wire into the reservoir is avoided. In addition, the analysis performance was enhanced compared with the former scheme, as demonstrated by the tests of standard peptides (gramicidin S, Glu-fibrinopeptide B). The limit of detection was determined to be lower than 10 Ϫ 2 M. Due to their enhanced performance, these microfabricated sources might be of great interest for analysis requiring very high sensitivity, such as proteomics analysis using nanoESI-MS. Proteomics studies are carried out on small amounts of sample and, thereby, need highly sensitive analysis techniques. ESI-MS matches this sensitivity criterion [2]. It also enables species identification using their precise molecular weights and provides fragment sequences using the MS/MS mode [1,3]. Therefore, ESI-MS is one of the best techniques for use in proteomics.Nanospraying is usually performed using glass or fused-silica-based capillary tips [2], and the spray is obtained by applying the ionization voltage to the electrical conductive tip or through the sample solution. However, capillary sources present a number of weaknesses: (1) a poorly controlled fabrication route resulting in a low reproducibility of the sources, (2) an irregular profile of the aperture after source opening, (3) easy clogging due to the small i.d., (4) signal suppression caused by air bubbles, and (5) deterioration of the conductive coating [4,5] upon operation.Different improvements regarding the quality of the emitter tip are proposed in the literature. Reproducible dimensions can be achieved using an enhanced fabrication route based on laser-micromachining [6,7]. More stable coatings have also been reported [8 -10]. Most of these improvements, however, do not solve the basic problems of clogging and the lack of reproducibility that affect the analysis conditions. Using microtechnology techniques, reproducible and ready-to-use high-quality nanospray tips can be produced in large batches. The tip geometry can be chosen to allow on-chip integration together with other microfluidic components, e.g., a chromatography column, and also to be compatible with the use of standard robots. Microfabrication of ESI emitter tips follows two main approaches: (1) a "needle-like" ap- Our approach is to design an em...

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“…The potential success of analytical systems also lies in the low production costs, opening the way for single use and contamination-free analytical devices. One particular category of microfluidic systems that shows promise in proteomics is microfabricated micro-or nanospray emitters [13][14][15][16][17][18][19][20]. First, microfabrication robustness and reproducibility make available reliable micro-and nanospray emitters, with automation possibilities [21].…”

mentioning

confidence: 99%

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

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We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting. (J Am Soc Mass Spectrom 2004, 15, 1767-1779

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Thin‐Chip Microspray System for High‐Performance Fourier‐Transform Ion‐Cyclotron Resonance Mass Spectrometry of Biopolymers

Cited by 60 publications

References 33 publications

Why the move to microfluidics for protein analysis?

Why the move to microfluidics for protein analysis?

An open design microfabricated nib-like nanoelectrospray emitter tip on a conducting silicon substrate for the application of the ionization voltage

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

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