Thin-layer chromatography analysis of fructooligosaccharides in biological samples

Reiffová, Katarína; Nemcová, Radomí­ra

doi:10.1016/j.chroma.2006.01.039

Cited by 53 publications

(25 citation statements)

References 17 publications

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“…The state of ITP in many fields was reviewed by many authors [97 -118] [190,191] Natural neurotoxins [192,193] Aromatic and aliphatic sulfonates [194,195] Pharmaceuticals [85, 86, 196 -205] Coupling of ITP with NMR [190,192,196,[232][233][234][235][236][237] [260] Gebauer and Bocek [119] was published in 2002. From this date, over a hundred novel applications and methods were published; a short survey of these works is given in Table 2.…”

Section: Recent Progress In Itpmentioning

confidence: 99%

Capillary isotachophoresis from the student point of view – images and the reality

et al. 2006

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Section: Recent Progress In Itpmentioning

confidence: 99%

Capillary isotachophoresis from the student point of view – images and the reality

et al. 2006

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“…The detection reagent was prepared by dissolving diphenylamine (2 g) and aniline (2 mL) in acetone (80 mL), carefully adding 15 mL of phosphoric acid and diluting to 100 mL with acetone. 100 mL of the detection reagent was used as dipping solution (Reiffova & Nemcova, 2006).…”

Section: Preparation Of Standards and Detection Reagentmentioning

confidence: 99%

“…Thin layer chromatography analyses of the plant extracts were carried out as described by Reiffova and Nemcova (2006) with slight modifications. Prior to TLC analysis, chromatographic silica gel 60 F 254 (200 × 200 mm) pre-coated aluminum sheets (Merck KGaA, Germany) were pre-treated with 0.02 M sodium acetate, followed by drying in an oven at 50 C for 5 min.…”

Section: Qualitative Thin Layer Chromatography (Tlc) Analysismentioning

confidence: 99%

Selected Sri Lankan food plants and other herbs as potential sources of inulin-type fructans

Mudannayake¹,

Wimalasiri²,

Silva³

et al. 2015

J. Natn. Sci. Foundation Sri Lanka

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Abstract:The objective of this study was to determine the inulin-type fructan content in 20 selected food plants and other herbs commonly found in Sri Lanka. The inulin content of the selected plants were determined qualitatively and quantitatively using thin layer chromatography (TLC) and enzymatic spectrophotometric (ES) methods, respectively. The ES results showed that the inulin-type fructan contents based on fresh weight was highest in Allium sativum (18.62 % ± 1.55), followed by Asparagus falcatus (17.74 % ± 2.92), Asparagus racemosus (11.8 3% ± 0.87), Allium cepa (8.60 % ± 0.88), Allium ampeloprasum (6.20 % ± 0.23), Taraxacum javanicum (5.77 % ± 1.53) and Vernonia cinerea (4.55 % ± 0.93), respectively. Taraxacum javanicum and Vernonia cinerea plant extracts developed distinct blue black spots with the detection reagent on TLC plates similar to chicory inulin standard. However, Allium ampeloprasum, Allium cepa, Allium sativum, Asparagus falcatus and Asparagus racemosus developed thicker blue black streaks on TLC plates due to their higher inulin concentration, which confirmed the ES results. Aloe vera, Alpinia calcarata, Amophophallus campanulatus, Beta vulgaris, Canna indica, Diascorea alata and Sonchus oleraceus FW) of inulin while Caryota urens, Ipomoea batatas, Lasia spinosa and Maranta arundinacea contained very low levels or no (< 0.4 g/100 g FW) inulin.

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“…All the bacterial isolates that exhibited considerable hydrolytic zone were subjected to secondary screening. In this step, the action pattern of inulinase was studied by thin layer chromatography (TLC) (Reiffová & Nemcová 2006). Crude enzyme (1.0 mL) was mixed with an equal volume of substrate solution (2% inulin in 0.1 M sodium acetate buffer, pH 5.5) and incubated at 50…”

Section: Collection Of Soil Samplesmentioning

confidence: 99%

Molecular and biochemical characterization of a new endoinulinase producing bacterial strain of Bacillus safensis AS-08

Singh

Yadav

2013

Biologia

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Microbial inulinases are an important class of industrial enzymes, which are used for the production of fructooligosaccharides and high-fructose syrup. Endoinulinase producing bacterial strains were isolated from soil samples taken from the vicinity of Asparagus sp. root tubers. All the bacterial strains were screened for inulinase activity. The primary screening was carried out based on hydrolytic zone on agar plates containing inulin-based medium and Lugol's iodine solution. Thus 30 inulinase producing bacterial strains were isolated. Out of 30 strains, 5 bacterial strains were found endoinulolytic, whereas 25 were exoinulolytic on the basis of action pattern of the enzyme. In tertiary screening, the bacterial isolate AS-08 was found to be most efficient for inulinase activity. Morphological, biochemical and physiological characteristics of the bacterial isolate AS-08 confirmed it as Bacillus sp. Furthermore, species-specific identification by 16S rDNA sequencing and phylogenetic analysis revealed the isolate as Bacillus safensis. Bacillus pumilus SH-B30 was found to be the nearest homolog. The strain showed maximum inulinase activity (12.56 U/mL) after 20 h of incubation at 37

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Thin-layer chromatography analysis of fructooligosaccharides in biological samples

Cited by 53 publications

References 17 publications

Capillary isotachophoresis from the student point of view – images and the reality

Capillary isotachophoresis from the student point of view – images and the reality

Selected Sri Lankan food plants and other herbs as potential sources of inulin-type fructans

Molecular and biochemical characterization of a new endoinulinase producing bacterial strain of Bacillus safensis AS-08

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