Thin-layer chromatography and densitometry in drug assay: Comparison of methods for monitoring valproic acid in plasma

Corti, Piero; Cenni, A.; Corbini, Gianfranco; Dreassi, Elena; Murratzu, C; Caricchia, A.M.

doi:10.1016/0731-7085(90)80071-v

Cited by 7 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…conjugates) or for complex mixtures (Kay and Warren, 1965;Neher, 1969;Wustermann et al, 1964). Hence, reversed phase systems generated by silanizing silica gel (Corti et al, 1984;Draffehn et al, 1981) or impregnating silica gel with hydrocarbons (Michalec et al, 1962;Copius-Peereboom and Beekes, 1962) have been described. Furthermore, the separation mechanism with cellulose and kieselguhr frequently involves considerable partition (Bennett and Heftmann, 1962;Carreras Matas, 1960;Neher, 1964).…”

Section: Paper and Thin Layer Chromatographymentioning

confidence: 99%

Chromatography as a reference technique for the determination of clinically important steroids

Robards

Towers

1990

Biomedical Chromatography

View full text Add to dashboard Cite

Chromatographic methods (paper chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry) for the determination of clinically important steroids in biological specimens are reviewed. The emphasis is on the use of gas chromatography, gas chromatography-mass spectrometry and high performance liquid chromatography as reference rather than routine techniques. Chromatographic methods are compared with colorimetric, fluorimetric and radioimmunoassay procedures in terms of simplicity of operation, cost and ability to analyse large numbers of specimens. The importance of correct specimen collection and storage are discussed. Sample preparation techniques for the various analytical methods are described. These include extraction of free and conjugated steroids from serum, plasma, urine and saliva by solvent partition, with polymer-based resins such as Amberlite XAD-2, DEAE-Sephadex and Sephadex resins bonded with various other function groups and, more recently, with chemically bonded reversed-phase silicas.

show abstract

Section: Paper and Thin Layer Chromatographymentioning

confidence: 99%

Chromatography as a reference technique for the determination of clinically important steroids

Robards

Towers

1990

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…The spectrophotometric determination ofnorethinodrel is based on its isomerization to norethisterone [ 11,17].…”

Section: 3%mentioning

confidence: 99%

Methods of control and standardization of drugs containing progestogen hormones (A review)

et al. 1998

View full text Add to dashboard Cite

The group of progestogen hormones includes progesterone (corpus luteum hormone) and a number of synthetic preparations with a structure close to that of progesterone (norethisterone, lynestrenol, levonorgestrel, desogestrel, etc.).The process of state control of the commercial progestogen hormonal preparations and the corresponding normative documentation are not free of significant drawbacks (in particular, some documents operative in the Russian Federation do not regulate the contents of related steroids). For this reason, the problems pertaining to the increase in the level of standardization and control of the quality of progestogen hormonal preparations require permanent attention and thorough consideration.A lot of information concerning the analysis of progestogen hormones is contained in the monograph of G6rrg [1]. However, there are some new data accumulated since that publication.The main methods used for the analysis of progesterone and related drugs were reviewed in [2].An analysis of the published data shows that the authenticity of ethisterone [3][4][5], norethisterone [4,5], and hydroxyprogesterone capronate [5] is established using a color reaction with sulfuric acid; levonorgestrel is identified by its reaction with m-dinitrobenzene in alkaline medium [5]; ethis-I State Research Institute of Drug Standardization and Control, Ministry of Health and Medical Industry, Moscow, Russia.

show abstract

“…They are useful in monitoring the therapeutic concentration of a substance, treatment progress, and side effects. Several methods for determination of valproic acid are known, namely: thin layer chromatography [5], liquid chromatography [6][7][8][9], high performance liquid chromatography [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], ultra high performance liquid chromatography [4,25,26], ultra performance liquid chromatography [27][28][29][30][31], gas chromatography [4,18,[32][33][34][35][36][37][38], capillary electrophoresis [39,40], voltamperometry [41], valproateselective electrode [42], and colorimetry [43].…”

Section: Introductionmentioning

confidence: 99%

“…The vast majority of valproic acid was determined in biological samples [4][5][6][7][8][9][10][11][12][13]16,[19][20][21][22][24][25][26][27][28][29][30][31][32][33][34][35][36][38][39][40]. Only a few studies report the determination of valproic acid in pharmaceutical preparations [14,17,20,23,35,37,41,42].…”

Section: Introductionmentioning

confidence: 99%

“…The disadvantage of chromatographic methods is that substances must be isolated from biological material and separated. Until now, only one paper concerning determination of valproic acid in plasma by TLC has been published in the available scientific literature [5]. There are no studies of the use of TLC in the quantitative analysis of valproic acid in pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of TLC-Densitometric Method for Determination of Valproic Acid in Capsules

Parys

Pyka

2022

Molecules

View full text Add to dashboard Cite

Determination of valproic acid in the drug was carried out on the aluminum silica gel 60F254 plates and using acetone–water–chloroform–ethanol–ammonia at a volume ratio of 30:1:8:5:11 as the mobile phase, respectively. Two methods of detection of valproic acid were used. The first was a 2% aqueous CuSO4×5H2O solution, and the second was a 2′,7′-dichlorofluorescein-aluminum chloride-iron (III) chloride system. The applied TLC-densitometric method is selective, linear, accurate, precise, and robust, regardless of the visualizing reagent used for the determination of valproic acid in Convulex capsules. It has low limits of detection (LOD) and limits of quantification (LOQ), which are equal to 5.8 μg/spot and 17.4 μg/spot using a 2% aqueous CuSO4×5H2O solution as visualizing agent and also 0.32 μg/spot and 0.97 μg/spot using a 2′,7′-dichlorofluorescein-aluminum chloride-iron (III) chloride system as visualizing reagent, respectively. The described analytical method can additionally be used to study the identity of valproic acid in a pharmaceutical preparation. The linearity range was found to be 20.00–80.00 μg/spot and 1.00–2.00 μg/spot for valproic acid detected on chromatographic plates using a 2% aqueous CuSO4×5H2O solution and the 2′,7′-dichlorofluorescein-aluminum chloride-iron (III) chloride system, respectively. A coefficient of variation that was less than 3% confirms the satisfactory accuracy and precision of the proposed method. The results of the assay of valproic acid equal 96.2% and 97.0% in relation to the label claim that valproic acid fulfill pharmacopoeial requirements. The developed TLC-densitometric method can be suitable for the routine analysis of valproic acid in pharmaceutical formulations. The proposed TLC-densitometry may be an alternative method to the modern high-performance liquid chromatography and square wave voltammetry in the control of above-mentioned substances, and it can be applied when other analytical techniques is not affordable in the laboratory.

show abstract

Thin-layer chromatography and densitometry in drug assay: Comparison of methods for monitoring valproic acid in plasma

Cited by 7 publications

References 9 publications

Chromatography as a reference technique for the determination of clinically important steroids

Chromatography as a reference technique for the determination of clinically important steroids

Methods of control and standardization of drugs containing progestogen hormones (A review)

Use of TLC-Densitometric Method for Determination of Valproic Acid in Capsules

Contact Info

Product

Resources

About