Thin-Layer Chromatography—Densitometry and Thin-Layer Chromatography—Image Analysis for Screening Bile Acid-Binding Activities of Thai Edible Plants

Sakunpak, Apirak; Suksaeree, Jirapornchai; Pathompak, Pathamaporn; Charoonratana, Tossaton; Chankana, Natawat; Sermkaew, Namfa

doi:10.1556/1006.2015.28.5.6

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…Nevertheless, the most potent was observed with P. merkusii heartwood extract [2]. The phytochemical constituents include phenolic compounds, flavonoids, terpenoids and resin acid [3,4]. Thus, P. merkusii could be a new natural product source of anti-S. mutans applied in pharmaceutical or cosmetic products such as oral health care sprays.…”

Section: Introductionmentioning

confidence: 99%

HPLC-DAD method validation for quantification of dehydroabietic acid and abietic acid in oral spray containing Pinus merkusii heartwood extract and its antibacterial effects on clinically isolated Streptococcus mutans

Sakunpak,

Saingam

2023

AChrom

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Pinus merkusii Jungh & De Vries. has become increasingly gathered more attention from researchers because the plant has a range of folk medicinal uses. Heartwood plant is the major source of dehydroabietic acid (DHAA) and abietic acid (AA), which possesses several medicinal properties, such as antiviral, antimicrobial, antiobesity and anti-inflammatory. The research proposed herein a low-cost, fast, specific, uncomplicated, sensitive, precise reverse-phase high-performance liquid chromatography (RP-HPLC). This method was conducted and validated for evaluating an amount of DHAA and AA in ethanol extract and oral spray containing P. merkusii heartwood extract. Additionally, stability and antimicrobial activities against clinically isolated Streptococcus mutans of the oral spray were determined. The separation was achieved on Pursuit 200Å PFP column, 150 × 4.6 mm, particles of 3 µm with a flow rate of 1.0 mL min−1. Methanol and water (70:30 v/v) were used as eluent with an isocratic mode and sample analysis volume was set at 10 µL, at a detection wavelength of 210 and 245 nm. The developed HPLC method for analysis of DHAA and AA showed good linearity with correlation coefficients equal to 1. Moreover, other validation parameters, comprised of accuracy, precision, specificity and detection and quantitation limits of this method displayed excellent reliability, validity and sensitivity. This method could be an interesting alternative for quantitative measurement of P. merkusii heartwood extract, oral spray formulation and other P. merkusii heartwood extract preparations. The result from antibacterial tests suggested that the oral spray containing P. merkusii heartwood extract is able to inhibit the oral pathogens causing dental caries. The oral spray decreased S. mutans population size by about 0.5–2 Log CFU mL−1 at 1–4 h and complete elimination of all bacteria strains within 24 h. This study provides validity for using P. merkusii heartwood extract as an alternative for preventing and treating oral infectious diseases.

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Section: Introductionmentioning

confidence: 99%

HPLC-DAD method validation for quantification of dehydroabietic acid and abietic acid in oral spray containing Pinus merkusii heartwood extract and its antibacterial effects on clinically isolated Streptococcus mutans

Sakunpak,

Saingam

2023

AChrom

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“…To identify new candidate compounds we will investigate plant sources that have established uses as homeopathic remedies. Vietnamese coriander ( Polygonum odoratum ) is a south-east Asian herb (Sakunpak et al., 2015) with homeopathic medicinal qualities (Fujita et al., 2015) including the treatment of stomach pains (Corlett et al., 2003), acting as an anti-inflammatory and has also been shown to have anti-bacterial activity (Ayaz et al., 2015; Ayaz et al., 2016; Ayaz et al., 2017). Additionally, P. ordoratum plant extracts are known to have high levels of antioxidant compounds.…”

Section: Introductionmentioning

confidence: 99%

Polygonum odoratum essential oil inhibits the activity of mushroom derived tyrosinase

Murray

Satooka

Shimizu

et al. 2019

Heliyon

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Plant derived compounds are a source of long term research focus due to their applications in a variety of fields, particularly food preservation. One key way in which phytochemicals are crucial in this area is by disrupting enzyme functionality. In this work, essential oil was extracted by steam distillation from the fresh leaves of Polygonum odoratum (Polygonaceae), commonly known as Vietnamese coriander, and shown to effectively inhibit the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase (EC1.14.18.1). Using GC-MS analysis, twenty five compounds were identified in the essential oil. The most abundant compounds in the essential oil were Alkanals - dodecanal (55.49%), and decanal (11.57%) - followed by anisaldehyde (6.35%); these compounds were individually investigated for inhibitory activity by performing single-compound screening. Each of the top three most abundant compounds inhibited the tyrosinase-catalyzed oxidation of L-DOPA, as identified by UV-VIS spectroscopy and oxygen consumption assays. The inhibitory activity of the major compounds increased when pre-incubated with tyrosinase and without significant additional oxygen consumption, suggesting kcat-type inactivation is not involved. Interactions of the head and tail components of the major alkanals may disrupt the tertiary structure of the enzyme, presenting a potential inhibitory mechanism.

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“…9 This software converts the spots in TLC plates into peaks and then the relationship between the peak area and spot density (i.e., amount of analyte) can be determined easily. [10][11][12] Quantitative analysis is also possible by analyzing the red, green, blue (RGB) values of TLC spots in Microsoft Paint program. 13 Liquid-liquid extraction (LLE) is a widely used sample preparation and pre-concentration method for the analysis of various analytes by TLC.…”

mentioning

confidence: 99%

Coupling Microextraction With Thin Layer Chromatography-Image Processing Analysis: A New Analytical Platform for Drug Analysis

Jain¹,

Singh²,

Sudhaker³

et al. 2017

Toxicol Forensic Med Open J

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Thin-Layer Chromatography—Densitometry and Thin-Layer Chromatography—Image Analysis for Screening Bile Acid-Binding Activities of Thai Edible Plants

Cited by 6 publications

References 15 publications

HPLC-DAD method validation for quantification of dehydroabietic acid and abietic acid in oral spray containing Pinus merkusii heartwood extract and its antibacterial effects on clinically isolated Streptococcus mutans

HPLC-DAD method validation for quantification of dehydroabietic acid and abietic acid in oral spray containing Pinus merkusii heartwood extract and its antibacterial effects on clinically isolated Streptococcus mutans

Polygonum odoratum essential oil inhibits the activity of mushroom derived tyrosinase

Coupling Microextraction With Thin Layer Chromatography-Image Processing Analysis: A New Analytical Platform for Drug Analysis

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