Thin-layer chromatography of phospholipids

Acetoacetate and glucose as substrates for lipid synthesis by rat brain oligodendrocytes and astrocytes in serum-free culture

“…An aliquot of lipid isolated from rat brain was added as carrier and total lipids were isolated according to Bligh and Dyer [20]. The incorporation of label into different lipid classes was analysed by thin-layer chromatography [21] 0I-I for 1 h at y neutralization and freeze-drying to ethanol. Cultme media were pre fro113 their components (Sigma, St. Louis, because of the high glucose content of commercially available media (5-E n-M).…”

Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System

“…Lipid extracts were solubilized in methanol and treated for 1 h at 80°C in presence or in absence of 2.5 M KOH. B, The mixture SG 1 GluCER from Medicago PMs and DIMs was first purified by HP-TLC (Vitiello and Zanetta, 1978) and further separated each from another by a second HP-TLC according to Hillig et al (2003). Tobacco GluCER was used as previously characterized standards (Mongrand et al, 2004), mainly composed of glucosylceramide with 4,8-sphingadiene (d18:2 D14,18 ) and hydroxypalmitic acid (gluCER1) and of lower amounts of GluCer species with the same backbone but with h18:0, h22:0, and h24:0 fatty acyl chains (gluCER2).…”

Role of cardiolipin on tBid and tBid/Bax synergistic effects on yeast mitochondria

“…Polar lipids were resolved by onedimensional thin layer chromatography on 10 Â 10 cm silica gel plates (Merck) using the solvent system as described. 48 Lipids were located by spraying the plates with a solution of 0.001% primuline in 80% acetone, visualized under UV light, scrapped and quantified by UV absorbance versus known standards.…”