Thin-layer chromatography. Reagents and detection methods. Physical and chemical detection methods: fundamentals, reagents I. Volume 1a

Jork, H.; Funk, W.; Fischer, W.; Wimmer, H.; Burns, D. Thorburn

doi:10.1016/s0003-2670(00)83962-8

Cited by 44 publications

(19 citation statements)

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“…The developing distance was 8 cm in all cases. All the compounds were detected in fluorescence mode with fluorescamine reagent according to the method described in [].…”

Section: Methodsmentioning

confidence: 99%

Comprehensive evaluation of lipophilicity of biogenic amines and related compounds using different chemically bonded phases and various descriptors

Casoni

Sârbu

2012

J of Separation Science

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The retention behavior for a series of biogenic amines and related sympathomimetic drugs has been investigated in reversed-phase thin-layer chromatography using RP-2, RP-8, RP-18W, and Diol stationary phase and mixtures of phosphate buffer (pH = 7.10) and methanol in different proportions as mobile phases. Several methodologies like arithmetic mean of experimental retention values, extrapolation to zero methanol concentration procedure and principal component analysis were applied to retention data values (R(M)) in order to determine relevant parameters (mean of R(M) - mR(M), R(M0), and scores corresponding to the first principal component - PC1/R(M) respectively) encoding information on the lipophilic behavior of compounds. High similarities in lipophilicity behavior of investigated amines were highlighted by mR(M) and PC1/R(M) lipophilicity indices for all of the studied stationary phases. The experimental results were compared with some computed lipophilicity parameters expressed as distribution coefficients at working pH (logD), partition coefficients (logP(N), logP(I), and diff(logP(N-I))) concerning both neutral and fully protonated species and difference between both species, and also with various lipophilicity values (logP) generated by different commonly used software. Significant correlations were observed between the experimental lipophilicity indices mR(M) respectively PC1/R(M) and diff(logP(N-I) ) values in all cases.

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“…The developing distance was 8 cm in all cases. All the compounds were detected in fluorescence mode with fluorescamine reagent according to the method described in [].…”

Section: Methodsmentioning

confidence: 99%

Comprehensive evaluation of lipophilicity of biogenic amines and related compounds using different chemically bonded phases and various descriptors

Casoni

Sârbu

2012

J of Separation Science

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“…[3] Barton's Reagent [3] Potassium hexacyanoferrate (1 g) (POCh, Poland) was dissolved in 100 mL water (solution I) and 2 g iron (III) chloride hexahydrate (POCh, Poland) was dissolved in 100 mL water (solution II). Immediately before use, 100 mL water, 8 mL solution I, 2 mL solution II, and 1 mL 32% hydrochloric acid (POCh, Poland) in a measuring cylinder were mixed and made up to 100 mL with methanol.…”

Section: Comparative Visualizing Reagentsmentioning

confidence: 99%

“…Rhodamine B Reagent [3,15] Rhodamine B (POCh, Poland) reagent was used as 50 mg/100 mL solutions in distillated water. Sulphuric Acid [3] A solution of sulphuric acid (VI) in methanol (1:19, v/v) was also applied for detection of estradiol.…”

Section: Comparative Visualizing Reagentsmentioning

confidence: 99%

“…Sulphuric Acid [3] A solution of sulphuric acid (VI) in methanol (1:19, v/v) was also applied for detection of estradiol.…”

Section: Comparative Visualizing Reagentsmentioning

confidence: 99%

“…The number of publications in the field of pharmacy steadily increases. [1,3] This results from the fact that contemporary thinlayer chromatography is a full instrumented and automated technique.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Visualizing Reagents for Estradiol on Thin Layer by Densitometric Method

Pyka

Klimczok

Gurak

2008

Journal of Liquid Chromatography & Related Technologies

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Five dyes as new visualizing reagents, namely gentian violet, methylene violet, methylene blue, malachite green, and Janus blue, have been used to detect estradiol on neutral aluminum oxide 60F 254 and on neutral aluminum oxide 150F 254 . Barton's reagent, rhodamine B, and sulphuric acid were used as the comparative visualizing reagents. Limit of detection (detectability), detection index, modified broadening index, modified contrast index, and linearity range were determined for estradiol following use of these visualizing reagents. It was stated, that the proposed modified contrast index is the objective parameter describing the applied visualizing reagents. The influence of a solid support on obtained visualizing effects was found. The angles (a) between the tangents at the inflection points to the curves of the densitometric peaks are more compact on neutral aluminum oxide 60F 254 than on neutral aluminum oxide 150F 254 . This observation indicates that the utility of any particular visualizing reagent depends on the type of chromatographic support used. For the quantitative research of estradiol investigated, relatively good properties had sulphuric acid (VI), Barton's reagent, gentian violet, and methylene violet.

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Antimicrobial activity and chemical composition of white birch (Betula papyrifera Marshall) bark extracts

et al. 2019

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More than 50 million tons of bark, mainly derived from pulp and wood industries, are produced annually in North America (Gupta, 2009). In Canada, only a fraction of the bark is used as an energy source by direct combustion, and the rest of the bark is incinerated or landfilled as waste (Cheng, Deng, Zhang, Riedl, & Cloutier, 2006). Both incineration and landfilling are nonsustainable avenues, whereas the combustion of bark is not ideal for energy production as it contains a high ash content that lower its heating values. Thus, the combustion of bark for energy recovery is not economically advantageous. In addition to ash, cellulose, hemicellulose, and lignin, bark also contains small amounts of bioactive compounds called extractives, which have potential to provide value-added coproducts

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Thin-layer chromatography. Reagents and detection methods. Physical and chemical detection methods: fundamentals, reagents I. Volume 1a

Cited by 44 publications

References 0 publications

Comprehensive evaluation of lipophilicity of biogenic amines and related compounds using different chemically bonded phases and various descriptors

Comprehensive evaluation of lipophilicity of biogenic amines and related compounds using different chemically bonded phases and various descriptors

Evaluation of Visualizing Reagents for Estradiol on Thin Layer by Densitometric Method

Antimicrobial activity and chemical composition of white birch (Betula papyrifera Marshall) bark extracts

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