Thiobarbituric Acid Reactive Material in Uremic Blood

Abstract

The thiobarbituric acid (TBA) assay was applied to uremic blood. Significant TBA-reactive material was found in patients with chronic renal failure compared to normal controls. The presence of TBA-reactive material correlated with the degree of renal failure, and was removed by hemodialysis. The reactive material is not 2-deoxyribose or sialic acid. In addition, the presence of TBA-reactive material in uremic blood is not abolished by prior aspirin ingestion, suggesting it is not related to active prostaglandin synthesis. The possible origin of the TBA-reactive material in uremic blood is discussed.

Red Blood Cell Membrane Lipid Peroxidation in Continuous Ambulatory Peritoneal Dialysis Patients

“…As in our patients, any interference by drugs with oxidizing proper ties can be excluded, a defective pentose-phosphate shunt is likely to be responsible. In a recent study, Fillit et al [16] suggested that the thiobarbituric-reactive material (TBA) found in the plasma of uremic patients may come from sources other than lipid peroxidation. Recent research [17] showed that in such patients administration of a-tocopheryl acetate causes a sharp decrease in the amount o f TBA-reactive material detectable in the RBC membranes.…”

Vascular Calcification

“…Furthermore, TNF-␣ enhances the DNA binding of Cbfa1/Runx2, activated protein 1, and cAMP responsive element binding protein, which are important transcription factors in osteoblastic differentiation. [81][82][83][84][85][86][87][88][89][90] In addition to the osteogenic transdifferentiation of VSMCs, Demer and colleagues 91,92 highlighted the contributions of pluripotent vascular mesenchymal progenitors to the vascular calcium accrual. Calcifying cells of Demer are mural multipotent mesenchymal progenitors related to the microvascular pericyte.…”

Effect of Tannins in Green Tea on the Urinary Methylguanidine Excretion in Rats Indicating a Possible Radical Scavenging Action

“…Fillit et al,5) Giardini et al,6) Kuroda et al, 7) and Flament et al 8 ) have also reported findings indicative of histological damage by active oxygen in patients with renal failure, suggesting the possibility that patients with renal failure are generally under oxidative stress.…”