Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis

Gao, Tong; Quanfei, Meng; Gao, Haichun

doi:10.1038/s41598-017-04285-5

Cited by 14 publications

(10 citation statements)

References 62 publications

(119 reference statements)

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“…Tol-Pal is required for cell motility in both P. putida and E. coli (Llamas, Ramos and Rodriguez-Herva 2000 ; Youderian et al . 2003 ; Gao, Meng and Gao 2017 ). In E. coli this has been shown to be due to recruitment of chemoreceptor clusters to cell poles (Santos et al .…”

Section: Tol-pal and Polar Localization Of Proteinsmentioning

confidence: 98%

The multifarious roles of Tol-Pal in Gram-negative bacteria

Szczepaniak

Press

Kleanthous

2020

FEMS Microbiology Reviews

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In the 1960s several groups reported the isolation and preliminary genetic mapping of Escherichia coli strains tolerant towards the action of colicins. These pioneering studies kick-started two new fields in bacteriology; one centred on how bacteriocins like colicins exploit the Tol (or more commonly Tol-Pal) system to kill bacteria, the other on the physiological role of this cell envelope-spanning assembly. The following half century has seen significant advances in the first of these fields whereas the second has remained elusive, until recently. Here, we review work that begins to shed light on Tol-Pal function in Gram-negative bacteria. What emerges from these studies is that Tol-Pal is an energised system with fundamental, interlinked roles in cell division – coordinating the re-structuring of peptidoglycan at division sites and stabilising the connection between the outer membrane and underlying cell wall. This latter role is achieved by Tol-Pal exploiting the proton motive force to catalyse the accumulation of the outer membrane peptidoglycan associated lipoprotein Pal at division sites while simultaneously mobilising Pal molecules from around the cell. These studies begin to explain the diverse phenotypic outcomes of tol-pal mutations, point to other cell envelope roles Tol-Pal may have and raise many new questions.

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Section: Tol-pal and Polar Localization Of Proteinsmentioning

confidence: 98%

The multifarious roles of Tol-Pal in Gram-negative bacteria

Szczepaniak

Press

Kleanthous

2020

FEMS Microbiology Reviews

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“…To investigate whether the plating defect is due to, at least in part, impaired cell envelope, we performed a comparative study with arcA and tolR mutants. In S. oneidensis , ArcA and TolR are a global regulator implicated in diverse processes and a protein involved in peptidoglycan recycling and cell division, respectively; the consequences caused by their loss are the severe defect in the outer-membrane and peptidoglycan layer ( Gao et al, 2008 , 2017 ). As a result, Δ arcA cells are very sensitive to SDS and Δ tolR cells form cell-chain with blebs ( Gao et al, 2017 ; Wan et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Oxidized OxyR Up-Regulates ahpCF Expression to Suppress Plating Defects of oxyR- and Catalase-Deficient Strains

et al. 2019

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It is well established that in bacteria, such as Escherichia coli, OxyR is a transcriptional regulator that mediates the response to H2O2 by activating the OxyR regulon, which consists of many genes that play vital roles in oxidative stress resistance. In Shewanella, OxyR regulates, however, in both reduced and oxidized states, the production of H2O2 scavengers, including major catalase KatB and NADH peroxidase AhpCF. Here we showed that the oxyR mutant carried a plating defect manifested as division arresting, a phenotype that can be completely suppressed by an OxyR variant constitutively existing in oxidized form (OxyRL197P). This effect of OxyRL197P could not be solely attributed to the increment in KatB production, since the suppression was also observed in the absence of KatB. Although expression of peroxidase CcpA was greatly activated by OxyRL197P, the contribution of the protein in alleviating plating defect was negligible. We eventually identified AhpCF as the critical factor, when produced at substantially elevated levels by OxyRL197P, to protect the cell from H2O2 attack. Our data indicate that AhpCF is a particularly important peroxidase in oxidative stress resistance in Shewanella, not only playing a compensatory role for catalase, but also by itself providing sufficient protection from killing of H2O2 generated abiotically.

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“…ybgC proteins are part of the Tol-Pal system that maintains cell membrane integrity (Angelini et al, 2008;Qin et al, 2019). It was also shown to be linked to bacterial motility (Gao et al, 2017), affect the Tol-Pal system and play a crucial role in Salmonella enteritidis survival under egg white stress (Qin et al, 2019). It is reported that, the transcriptional regulator in the MerR family can respond upon the various environmental stimuli, including antibiotics, heavy metals, and oxidative stress (Brown et al, 2003;Ghosh et al, 2017).…”

Section: Positive Selection Detection On Core Orthologous Genesmentioning

confidence: 99%

Characterization of Burkholderia cepacia Complex Core Genome and the Underlying Recombination and Positive Selection

Zhou¹,

Ren²,

Hu³

et al. 2020

Front. Genet.

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Recombination and positive selection are two key factors that play a vital role in pathogenic microorganisms' population adaptation and diversification. The Burkholderia cepacia complex (Bcc) represents bacterial species with high similarity, which can cause severe infections among cases suffering from the chronic granulomatous disorder and cystic fibrosis (CF). At present, no genome-wide study has been carried out focusing on investigating the core genome of Bcc associated with the two evolutionary forces. The general characteristics of the core genome of Bcc species remain scarce as well. In this study, we explored the core orthologous genes of 116 Bcc strains using comparative genomic analysis and studied the two adaptive evolutionary forces: recombination and positive selection. We estimated 1005 orthogroups consisting entirely of single copy genes. These single copy orthologous genes in some Cluster of Orthologous Groups (COG) categories showed significant differences in the comparison of several evolutionary properties, and the encoding proteins were relatively simple and compact. Our findings showed that 5.8% of the core orthologous genes strongly supported recombination; in the meantime, 1.1% supported positive selection. We found that genes involved in protein synthesis as well as material transport and metabolism are favored by selection pressure. More importantly, homologous recombination contributed more genetic variation to a large number of genes and largely maintained the genetic cohesion in Bcc. This high level of recombination between Bcc species blurs their taxonomic boundaries, which leads Bcc species to be difficult or impossible to distinguish phenotypically and genotypically.

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Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis

Cited by 14 publications

References 62 publications

The multifarious roles of Tol-Pal in Gram-negative bacteria

The multifarious roles of Tol-Pal in Gram-negative bacteria

Oxidized OxyR Up-Regulates ahpCF Expression to Suppress Plating Defects of oxyR- and Catalase-Deficient Strains

Characterization of Burkholderia cepacia Complex Core Genome and the Underlying Recombination and Positive Selection

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