Thiol‐based copper handling by the copper chaperone Atox1

Hatori, Yoshinori; Inouye, Sachiye; Akagi, Reiko

doi:10.1002/iub.1620

Cited by 46 publications

(35 citation statements)

References 84 publications

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“…Copper is a potent redox catalyst in removal of superoxide, whereas superfluous copper can cause oxidative damage because of its inherent oxidoreduction activity (33). ATOX1 has been reported to be an antioxidant and a copper chaperone that can bind and transfer copper and stimulate their catalytic activity (33). MTs, a family of metal binding and absorbing molecules, are important in detoxification of heavy metals and scavenging of free radicals (34).…”

Section: Discussionmentioning

confidence: 99%

“…IDH1 is a catalyzing enzyme that converts isocitrate and NADP + to a-ketoglutarate and NADPH and plays important roles in scavenging ROS (31,32). ATOX1 is an antioxidant and a copper chaperone (33). MT2A is a member of MT, a family that has been evidenced to be important in detoxification of heavy metals and scavenging of free radicals (34).…”

Section: Frumenti Facilitates the Antioxidant Capacity In Porcine mentioning

confidence: 99%

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Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

Nie

Hou

et al. 2019

FASEB j.

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Oxidative damages have adverse effects on mammals. Growing studies have focused on exploring new antioxidants. Here, we report that Lactobacillus frumenti increases the total antioxidation capacity activities and decreases the total reactive oxygen species levels in porcine intestinal epithelial cells. Comparative proteomics revealed that expressions of peroxiredoxin 2, isocitrate dehydrogenase 1, NAD(P)H dehydrogenase quinone 1, antioxidant protein 1, and metallothionein‐2A, which are associated with antioxidant defense system, were significantly increased with L. frumenti treatment. In germ‐free mice, L. frumenti treatment also remarkably improves the intestinal antioxidant capacity. We further illustrated that nitric oxide production—mediated by nitric oxide synthase 1 activation is essential for L. frumenti—induced improvements in intestinal epithelial antioxidant capacity and barrier function. This study suggested that L. frumenti may be a potential probiotic used to prevent oxidative stress—induced aging and diseases in mammals.—Nie, Y., Hu, J., Hou, Q., Zheng, W., Zhang, X., Yang, T., Ma, L., Yan, X. Lactobacillus frumenti improves antioxidant capacity via nitric oxide synthase 1 in intestinal epithelial cells. FASEB J. 33, 10705–10716 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

Section: Frumenti Facilitates the Antioxidant Capacity In Porcine mentioning

confidence: 99%

Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

Nie

Hou

et al. 2019

FASEB j.

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“…As described earlier, it was observed that Cu‐(II) addition to the CD CFM did not change the Trp‐ox levels, suggesting its effect of lowering Trp‐ox occurred intracellularly and via gene regulation mechanisms, such as elevating the expression of Atox1, Cox17, and MT1. Atox1 is a copper metallochaperone and also an antioxidant . It is capable of binding free metals in vivo to protect cells from generation of ROS and against hydrogen peroxide‐induced oxidative damage .…”

Section: Discussionmentioning

confidence: 99%

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

Desai

Gao

et al. 2018

Biotechnology Journal

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Oxidation of monoclonal antibodies (mAb) is a common chemical modification with potential impact on a therapeutic protein's activity and immunogenicity. In a previous study, it was found that tryptophan oxidation (Trp-ox) levels of two mAb produced in Chinese hamster ovary (CHO) cells were significantly lowered by modifying cell culture medium/feed. In this study, transcriptome analysis by RNA-Seq is applied to further elucidate the underlying mechanism of those changes in lowering the Trp-ox levels. Cell samples from the 5L fed-batch conditions are harvested and subjected to RNA-Seq analysis. The results showed that the cell culture changes had little impact on neither the expression of the mAb transgenes nor genes related to glycosylation. However, those changes did significantly alter the expression of multiple genes (p-value ≤0.05 and absolute fold change ≥1.5 or adjusted p-value ≤0.1) involved in transport of copper, regulation of glutathione, iron storage, heme reduction, oxidative phosphorylation, and Nrf2-mediated antioxidative response. These findings suggest a key underlying mechanism in lowering Trp-ox levels by CDM was likely to be collectively controlling ROS levels through regulation of those genes' expression. This is the first example, to our knowledge, applying transcriptomic analysis to mechanistically understand the impact of cell culture on mAb oxidation.

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“…This would lead to higher production of peroxynitrite which may be further converted to NO 2 by peroxiredoxins and glutathione peroxidases which also participate in reduction of H 2 O 2 (60, 61) and these pathways are probably used preferentially by HCT116 cells which show higher expression of PRDX, TXN, GPX than Me45 cells. The other pathway for ONOO-reduction is interaction with transition metal centers (reviewed in 48) and Me45 cells show significantly higher levels than HCT116 cells of ATOX gene transcripts coding for copper chaperone (62,22) and of transcripts of thioredoxin-inhibiting protein TXNIP, suggesting that in Me45 cells interaction of ONOO - with transition metals may be dominating.…”

Section: Discussionmentioning

confidence: 99%

Cell type-specific differences in redox regulation and proliferation after low UVA doses

Ciesielska

Bil

Gajda

et al. 2018

Preprint

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21Ultraviolet A (UVA) radiation is harmful for living organisms but in low doses may 22 stimulate cell proliferation. Our aim was to examine the relationships between exposure 23 to different low UVA doses, cellular proliferation, and changes in cellular reactive 24 oxygen species levels. In human colon cancer (HCT116) and melanoma (Me45) cells 25 exposed to UVA doses comparable to environmental, the highest doses (30-50 kJ/m 2 ) 26 reduced clonogenic potential but some lower doses (1 and 10 kJ/m 2 ) induced 27 proliferation. This effect was cell type and dose specific. In both cell lines the levels of 28 reactive oxygen species and nitric oxide fluctuated with dynamics which were 29 influenced differently by UVA; in Me45 cells decreased proliferation accompanied the 30 changes in the dynamics of H 2 O 2 while in HCT116 cells those of superoxide. Genes 31 coding for proteins engaged in redox systems were expressed differently in each cell 32 line; transcripts for thioredoxin, peroxiredoxin and glutathione peroxidase showed 33 higher expression in HCT116 cells whereas those for glutathione transferases and 34 copper chaperone were more abundant in Me45 cells. We conclude that these two cell 35 types utilize different pathways for regulating their redox status. Many mechanisms 36 engaged in maintaining cellular redox balance have been described. Here we show that 37 the different cellular responses to a stimulus such as a specific dose of UVA may be 38 consequences of the use of different redox control pathways. Assays of superoxide and 39 hydrogen peroxide level changes after exposure to UVA may clarify mechanisms of 40 cellular redox regulation and help in understanding responses to stressing factors. 41 42 43 44 Ciesielska 3 45 46 48with a wavelength of 100-400 nm, invisible to human sight. The sun is a natural emitter 49 of UV divided into three main fractions UVA (315-400 nm), UVB (280-315 nm), and 50 UVC (100-280 nm), but most of this radiation is blocked by the atmosphere (1,2). UVA 51 constitutes the largest part (∼95%) of UV radiation that reaches the Earth's surface (3), 52whereas UVB represents only 4-5% (1). In irradiated humans UVA reaches the dermis 53 and hypodermis and has no direct impact on DNA, but it can influence cellular 54 structures indirectly by induction of reactive oxygen species (ROS) which can damage 55 macromolecules (4,1). For a long time UV was regarded as damaging for cells and 56 organisms (5), but since a few decades it is known that low doses can also stimulate 57 proliferation of cells; however, the mechanisms underlying this phenomenon are not 58 completely understood (3,1,6,7). 59Studies of signaling pathways in conditions where UVA stimulates cell proliferation 60 show changes in the levels of proteins engaged in controlling proliferation such as 61 cyclin D1 (8,9), Pin1 (3), and Kin17 (10) or activation of epidermal growth factor 62 receptor (EGFR) which is strongly mitogenic in many cell types (8). Experiments on 63 mice showed that UVA can accelerate tumor growth ...

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Thiol‐based copper handling by the copper chaperone Atox1

Cited by 46 publications

References 84 publications

Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

Cell type-specific differences in redox regulation and proliferation after low UVA doses

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