Thiol-Based Redox Modulation of a Cyanobacterial Eukaryotic-Type Serine/Threonine Kinase Required for Oxidative Stress Tolerance

Mata‐Cabana, Alejandro; García-Domínguez, Mario; Florencio, Francisco J.; Lindahl, Marika

doi:10.1089/ars.2011.4483

Cited by 25 publications

(14 citation statements)

References 48 publications

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“…The high copper treatment led to a ROS accumulation that was almost five times (4.7±0.6) higher than the low copper or control treatments ( Fig. 3C ) and similar to methyl viologen (5 µM) treated cells (3.1±0.3) [52] . All these data suggest that oxidative stress and protein damage likely mediated by copper generated ROS, are important features of the copper stress response in Synechocystis , as has been reported for other bacteria [53] , [54] , [55] , [56] .…”

Section: Resultsmentioning

confidence: 70%

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

2014

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Copper is an essential element involved in fundamental processes like respiration and photosynthesis. However, it becomes toxic at high concentration, which has forced organisms to control its cellular concentration. We have recently described a copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803, which is mediated by the two-component system, CopRS, a RND metal transport system, CopBAC and a protein of unknown function, CopM. Here, we report the transcriptional responses to copper additions at non-toxic (0.3 µM) and toxic concentrations (3 µM) in the wild type and in the copper sensitive copR mutant strain. While 0.3 µM copper slightly stimulated metabolism and promoted the exchange between cytochrome c6 and plastocyanin as soluble electron carriers, the addition of 3 µM copper catalyzed the formation of ROS, led to a general stress response and induced expression of Fe-S cluster biogenesis genes. According to this, a double mutant strain copRsufR, which expresses constitutively the sufBCDS operon, tolerated higher copper concentration than the copR mutant strain, suggesting that Fe-S clusters are direct targets of copper toxicity in Synechocystis. In addition we have also demonstrated that InrS, a nickel binding transcriptional repressor that belong to the CsoR family of transcriptional factor, was involved in heavy metal homeostasis, including copper, in Synechocystis. Finally, global gene expression analysis of the copR mutant strain suggested that CopRS only controls the expression of copMRS and copBAC operons in response to copper.

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Section: Resultsmentioning

confidence: 70%

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

2014

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“…Synechocystis presents 12 genes coding for Ser/Thr kinases (Spk), 134,135 of which seven are classified in the PKN2 subfamily (spkA to spkG), 136 and the rest have been assigned to the ABC1 subfamily (spkH to spkL). 137 These kinases have been shown to participate in different cell functions, 138 such as cell motility, 139 oxidative stress response, 140 salt and low temperature stress, 141,142 and regulation of carbon 143 and nitrogen metabolism. 144 In addition, the GroES chaperone protein and ferredoxin were also reported to be targets of some Spk.…”

Section: The Signal Transduction Pathways and The Role Of Cyt B 6 Fmentioning

confidence: 99%

Revisiting cyanobacterial state transitions

Calzadilla

Kirilovsky

2020

Photochem Photobiol Sci

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“…Some functions of Ser/Thr kinases (Spk) have already been described. For example, SpkA is involved in the control of cell motility (Kamei et al, 2001;Panichkin et al, 2006); SpkB participates in the oxidative stress response by phosphorylating glycyl-tRNAsynthetase b-subunit (Mata-Cabana et al, 2012); SpkE might be involved in the regulation of nitrogen metabolism (Galkin et al, 2003); SpkD might be involved in adjusting the pool of tricarboxylic acid cycle metabolites (Laurent et al, 2008); SpkG plays an essential role in high-salt resistance (Liang et al, 2011); SpkC, SpkF, and SpkK are involved in the phosphorylation of the GroES chaperone protein (Zorina et al, 2014); and SpkG is involved in the phosphorylation of ferredoxin 5 protein (Angeleri et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Cytochrome b₆f Complex Is Not Involved in Cyanobacterial State Transitions

et al. 2019

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Photosynthetic organisms must sense and respond to fluctuating environmental conditions in order to perform efficient photosynthesis and to avoid the formation of dangerous reactive oxygen species. The excitation energy arriving at each photosystem permanently changes due to variations in the intensity and spectral properties of the absorbed light. Cyanobacteria, like plants and algae, have developed a mechanism, named "state transitions," that balances photosystem activities. Here, we characterize the role of the cytochrome b 6 f complex and phosphorylation reactions in cyanobacterial state transitions using Synechococcus elongatus PCC 7942 and Synechocystis PCC 6803 as model organisms. First, large photosystem II (PSII) fluorescence quenching was observed in State II, a result that does not appear to be related to energy transfer from PSII to PSI (spillover). This membrane-associated process was inhibited by betaine, Suc, and high concentrations of phosphate. Then, using different chemicals affecting the plastoquinone pool redox state and cytochrome b 6 f activity, we demonstrate that this complex is not involved in state transitions in S. elongatus or Synechocystis PCC6803. Finally, by constructing and characterizing 21 protein kinase and phosphatase mutants and using chemical inhibitors, we demonstrate that phosphorylation reactions are not essential for cyanobacterial state transitions. Thus, signal transduction is completely different in cyanobacterial and plant (green alga) state transitions.

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Thiol-Based Redox Modulation of a Cyanobacterial Eukaryotic-Type Serine/Threonine Kinase Required for Oxidative Stress Tolerance

Abstract: The SpkB kinase is required for survival of the cyanobacterium Synechocystis sp. PCC 6803 under conditions implying increased concentrations of reactive oxygen species, and the activity of SpkB depends on the redox state of its cysteines.

Cited by 25 publications

References 48 publications

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

Revisiting cyanobacterial state transitions

The Cytochrome b₆f Complex Is Not Involved in Cyanobacterial State Transitions

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Thiol-Based Redox Modulation of a Cyanobacterial Eukaryotic-Type Serine/Threonine Kinase Required for Oxidative Stress Tolerance

Abstract: The SpkB kinase is required for survival of the cyanobacterium Synechocystis sp. PCC 6803 under conditions implying increased concentrations of reactive oxygen species, and the activity of SpkB depends on the redox state of its cysteines.

Cited by 25 publications

References 48 publications

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

Revisiting cyanobacterial state transitions

The Cytochrome b6f Complex Is Not Involved in Cyanobacterial State Transitions

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The Cytochrome b₆f Complex Is Not Involved in Cyanobacterial State Transitions