Thionin attached to a gold electrode modified with self-assembly of Mo6S9−I  nanowires for amplified electrochemical detection of natural DNA

Lin, Hong; Cheng, Huhu; Liu, Lu; Zhu, Zhiwei; Shao, Yuanhua; Papakonstantinou, Pagona; Mihailović, D.; Li, Meixian

doi:10.1016/j.bios.2010.01.035

Cited by 26 publications

(9 citation statements)

References 42 publications

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“…Compared to carbon nanotubes, MoSI NWs have some significant advantages such as straight forward synthesis, monodisperse diameters, and metallic properties [28][29][30][31][32][33]. Our preliminary work has demonstrated its application in fabricating novel electrochemical sensors for highly sensitive detection of estrone hapten [34], Hydrogen peroxide [35] and natural double-stranded deoxyribonucleic acid [36]. The results illustrate that MoSI NWs can act as a good sensing platform to construct highly sensitive and effective sensing devices.…”

Section: Introductionmentioning

confidence: 95%

Simultaneous Determination of Copper, Lead, and Cadmium Ions at a Mo 6 S 9-x I x Nanowires Modified Glassy Carbon Electrode Using Differential Pulse Anodic Stripping Voltammetry

Lin

Mihailović

2015

Electrochimica Acta

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Section: Introductionmentioning

confidence: 95%

Simultaneous Determination of Copper, Lead, and Cadmium Ions at a Mo 6 S 9-x I x Nanowires Modified Glassy Carbon Electrode Using Differential Pulse Anodic Stripping Voltammetry

Lin

Mihailović

2015

Electrochimica Acta

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“…B. napus hevein-like peptide sequences are about 30 amino acids to 45 amino acids long and possess eight unique Cys in a chitin-binding peptide with several strictly conserved residues, serine, and two glycines (Additional file 3 : Figure S4) [ 17 , 18 ]. B. napus thionin has six conserved Cys residues, as well as conserved proline and glycine residues (Additional file 3 : Figure S6) [ 19 , 20 ]. A variable number of Cys residues that helped stabilize conserved scaffolds through disulfide bond formation were found [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus

et al. 2015

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BackgroundBrassica napus is the third leading source of vegetable oil in the world after soybean and oil palm. The accumulation of gene sequences, especially expressed sequence tags (ESTs) from plant cDNA libraries, has provided a rich resource for genes discovery including potential antimicrobial peptides (AMPs). In this study, we used ESTs including those generated from B. napus cDNA libraries of seeds, pathogen-challenged leaves and deposited in the public databases, as a model, to perform in silico identification and consequently in vitro confirmation of putative AMP activities through a highly efficient system of recombinant AMP prokaryotic expression.ResultsIn total, 35,788 were generated from cDNA libraries of pathogen-challenged leaves and 187,272 ESTs from seeds of B. napus, and the 644,998 ESTs of B. napus were downloaded from the EST database of PlantGDB. They formed 201,200 unigenes. First, all the known AMPs from the AMP databank (APD2 database) were individually queried against all the unigenes using the BLASTX program. A total of 972 unigenes that matched the 27 known AMP sequences in APD2 database were extracted and annotated using Blast2GO program. Among these unigenes, 237 unigenes from B. napus pathogen-challenged leaves had the highest ratio (1.15 %) in this unigene dataset, which is 13 times that of the unigene datasets of B. napus seeds (0.09 %) and 2.3 times that of the public EST dataset. About 87 % of each EST library was lipid-transfer protein (LTP) (32 % of total unigenes), defensin, histone, endochitinase, and gibberellin-regulated proteins. The most abundant unigenes in the leaf library were endochitinase and defensin, and LTP and histone in the pub EST library. After masking of the repeat sequence, 606 peptides that were orthologous matched to different AMP families were found. The phylogeny and conserved structural motifs of seven AMPs families were also analysed. To investigate the antimicrobial activities of the predicted peptides, 31 potential AMP genes belonging to different AMP families were selected to test their antimicrobial activities after bioinformatics identification. The AMP genes were all optimized according to Escherichia coli codon usage and synthetized through one-step polymerase chain reaction method. The results showed that 28 recombinant AMPs displayed expected antimicrobial activities against E. coli and Micrococcus luteus and Sclerotinia sclerotiorum strains.ConclusionThe study not only significantly expanded the number of known/predicted peptides, but also contributed to long-term plant genetic improvement for increased resistance to diverse pathogens of B.napus. These results proved that the high-throughput method developed that combined an in silico procedure with a recombinant AMP prokaryotic expression system is considerably efficient for identification of new AMPs from genome or EST sequence databases.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1849-x) contains supplementary material, which is available to a...

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“…Fernandez-Ramos et al, found a similar behavior when developing a photometric sensor for nitrates [69]. By the way, this is a common behavior of chemical, optical [70][71][72] and electrochemical sensors [73][74][75][76][77]. The LOD was calculated using the following equation:…”

Section: Electrochemical Tests: Optimization and Calibrationmentioning

confidence: 88%

Copper nanowire array as highly selective electrochemical sensor of nitrate ions in water

Patella

Russo

O’Riordan

et al. 2021

Talanta

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Contamination of water with nitrate ions is a significant problem that affects many areas of the world. The danger from nitrates is not so much their toxicity, rather low, as their transformation into nitrites and in particular into nitrosamines, substances considered to be a possible carcinogenic risk. For this reason, European legislation has set the maximum permissible concentration of nitrates in drinking water at 44 mg/l. Thus, it is clear that a continuous monitoring of nitrate ions is of high technological interest but it must be rapid, easy to perform and directly performable in situ. Electrochemical detection is certainly among the best techniques to obtain the above requirements. In particular, in this work we have developed a nanostructured sensor based on array of copper nanowires obtained with the simple method of galvanic deposition. The nanostructured sensors have a very short response time with a detection limit less than 10 M. Different interfering species were tested finding a negligible effect except for the chlorine ions. However, this problem has been solved by removing chlorine ions from the water through a simple precipitation of chloride compounds with low solubility. Nanostructured sensors were also used to analyze real water samples (rain, river and drinking water). In the case of drinking water, we have measured a concentration of nitrate ions very close to the that measured by conventional laboratory techniques.

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Thionin attached to a gold electrode modified with self-assembly of Mo6S9−I nanowires for amplified electrochemical detection of natural DNA

Cited by 26 publications

References 42 publications

Simultaneous Determination of Copper, Lead, and Cadmium Ions at a Mo 6 S 9-x I x Nanowires Modified Glassy Carbon Electrode Using Differential Pulse Anodic Stripping Voltammetry

Simultaneous Determination of Copper, Lead, and Cadmium Ions at a Mo 6 S 9-x I x Nanowires Modified Glassy Carbon Electrode Using Differential Pulse Anodic Stripping Voltammetry

EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus

Copper nanowire array as highly selective electrochemical sensor of nitrate ions in water

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