Thiopental sodium loaded solid lipid nano-particles attenuates obesity-induced cardiac dysfunction and cardiac hypertrophy via inactivation of inflammatory pathway

Abstract

This work evaluates solid lipid nanoparticles of thiopental sodium against obesity-induced cardiac dysfunction and hypertrophy and explores the possible mechanism of action. TS loaded SLNs were formulated by hot-homogenization and solvent diffusion method. TS-SLNs were scrutinized for entrapment efficiency, drug loading capacity, gastric stability, particle size, in vitro drug release. Mice were feed with the normal chow or high-fat diet for 08 weeks to induce obesity and primary cardiomyocytes. The therapeutic effects of thiopental sodium in the high fat diet (HFD) induced cardiac hypertrophy. Systolic blood pressure (SBP) was estimated at a regular time interval. At the end of the experimental study, systolic pressure left ventricular, LV end-diastolic pressure and rate of increase of LV pressure and antioxidant, apoptosis, cytokines and inflammatory scrutinized. HFD induced group mice exhibited a reduction in the body weight and enhancement of cardiac hypertrophy marker and dose-dependent treatment of thiopental sodium up-regulation the body weight and down-regulated the cardiac hypertrophy. Thiopental sodium significantly (p < .001) dose-dependently altered the antioxidant, biochemical, cardiac parameters and remodeling. Thiopental sodium significantly (p < .001) dose-dependently reduced the SBP. Thiopental sodium altered the apoptosis marker, pro-inflammatory cytokines, inflammatory parameters along with reduced the p38-MAPK level. The cardiac protective effect of thiopental sodium shed light on future therapeutic interventions in obesity and related cardiovascular complications via inflammatory pathway.

Teucrium polium extract‐loaded solid lipid nanoparticles: A design and in vitro anticancer study

“…In the present study, stearic acid and lecithin were used as a lipid matrix and surfactant, respectively and in the aqueous phase, water was used along with Tween 80. Similarly, in different studies, stearic acid was used as a lipid matrix (Fazly Bazzaz et al., 2018; Singh et al., 2014; Trucillo & Campardelli, 2019), lecithin (Andrade et al., 2019; Singh et al., 2014; Trucillo & Campardelli, 2019) as a surfactant, and Tween 80 (Andrade et al., 2019; Zhu et al., 2020) as a co‐surfactant for the synthesis of SLN, which is comparable to the present study.…”

Biomaterial-Based Therapeutic Strategies for Obesity and Its Comorbidities

“…In addition, it also possesses anti-inflammatory properties. In a study of a high-fat diet-induced cardiac hypertrophy model in obese mice, it was found that thiopental sodium lipid nanoparticles attenuated cardiac damage, hypotension, and myocardial hypertrophy induced by obesity-induced cardiac insufficiency by suppressing the inflammatory pathway and also significantly altered cardiac remodeling function, aortic function, oxidative stress, and the inflammatory response [ 135 ]. The cardioprotective effect of sodium thiopental provides evidence for future treatment of obesity and related cardiovascular complications via inflammatory pathways.…”