Thiophilic paramagnetic particles as a batch separation medium for the purification of antibodies from various source materials

Thiophilic adsorption revisited☆

“…Conversely, it is possible to improved selective adsorption by adjusting the sample/support ratio, even if there will be some decrease of capacity. Taking that into account, qualities such as low gel costs, functional integrity of the immunoglobulins isolated on T-gel, and a purity similar to that obtained when using protein A beads [14], make that TAC remains an effective procedure for the isolation and purification of immunoglobulins.…”

“…The efficacy of T-gel to bind immunoglobulins was generally estimated by methods that does not provide high resolution, such as cellulose acetate electrophoresis [14], SDS-PAGE [15][16][17][18] or native PAGE [2,14], and fused rocket immunoelectrophoresis [7]. These methods provided a semi-quantitative estimation of the level of immunoglobulins in the eluates, but did not allow investigating the contaminants present in these eluates.…”

Using thiophilic magnetic beads in purification of antibodies from human serum

“…Thus, both adsorption and recovery efficiency were remarkably increased. If the particles were too large, it took about 20 h to reach adsorption equilibrium [14].…”

“…The advent of magnetic affinity separation techniques has opened up a new approach to antibody purification [12][13][14][15]. The rapid process of magnetic separation and specific fractionation of affinity chromatography are combined by this technology.…”

Interaction of pepsin with aromatic amino acids and their derivatives immobilized to Sepharose

“…Refs. [25][26][27][28][29][30][31][32][33]), but it could be also employed in the purification of other proteins [26,[33][34][35]. The thiophilic adsorption of proteins is based on an interaction with a sulfone group close to a thioether group of the T-gel ligand.…”