Thioredoxin1 regulates conidia formation, hyphal growth, and trap formation in the nematode-trapping fungus Arthrobotrys oligospora

Wang, Ji-ai; Huang, Xiaowei; Niu, Shanzhuang; Hú, Zhìhóng; Li, Heng; Ji, Xinglai; Yu, Hua; Zeng, Weikun; Tao, Jian; Chen, Weiwei; Jun, Li; Li, Juan; Zhang, Ke‐Qin

doi:10.1007/s13213-019-01511-5

Cited by 3 publications

(1 citation statement)

References 21 publications

(27 reference statements)

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“…The disruption vector of AOL_s00004g335 was constructed using a modified yeast cloning procedure (Li et al, 2019;Wang et al, 2019). The 2183 bp upstream homologous fragment and the 1956-bp downstream homologous fragment of AOL_s00004g335 were amplified via PCR using primer pairs 335-5f/5r, and 335-3f/3r, respectively (Supplementary Materials, Table S1).…”

Section: Aol_s00004g335 Knockout Vector Constructionmentioning

confidence: 99%

Methylglyoxal Has Different Impacts on the Fungistatic Roles of Ammonia and Benzaldehyde, and Lactoylglutathione Lyase Is Necessary for the Resistance of Arthrobotrys oligospora to Soil Fungistasis

Long

Tan

et al. 2021

Front. Cell. Infect. Microbiol.

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Biocontrol of root-knot nematode has attracted increasing attention over the past two decades. The inconsistent field performance of biocontrol agents, which is caused by soil fungistasis, often restricts their commercial application. There is still a lack of research on the genes involved in biocontrol fungi response to soil fungistasis, which is important for optimizing practical applications of biocontrol fungi. In this study, the lactoylglutathione lyase-encoding AOL_s00004g335 in the nematophagous fungi Arthrobotrys oligospora was knocked out, and three mutant strains were obtained. The hyphal growth of mutants on the three media was almost the same as that of the wild-type strain, but mutants had slightly higher resistance to NaCl, SDS, and H2O2. Methylglyoxal (MG) significantly increased the resistance of A. oligospora to ammonia, but decreased the resistance to benzaldehyde. Furthermore, the resistance of the mutants to soil fungistasis was largely weakened and MG could not increase the resistance of A. oligospora to soil fungistasis. Our results revealed that MG has different effects on the fungistatic roles of ammonia and benzaldehyde and that lactoylglutathione lyase is very important for A. oligospora to resist soil fungistasis.

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Section: Aol_s00004g335 Knockout Vector Constructionmentioning

confidence: 99%

Methylglyoxal Has Different Impacts on the Fungistatic Roles of Ammonia and Benzaldehyde, and Lactoylglutathione Lyase Is Necessary for the Resistance of Arthrobotrys oligospora to Soil Fungistasis

Long

Tan

et al. 2021

Front. Cell. Infect. Microbiol.

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The fucose-specific lectin gene AOL_s00054g276 affects trap formation and nematocidal activity of the nematophagous fungus Arthrobotrys oligospora

Wang

2022

FEMS Microbiology Letters

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Nematode-trapping fungi are natural enemies of nematodes in nature. Arthrobotrys oligospora, a typical nematode-trapping fungus with a clear genetic background, can capture and infect nematodes by forming adhesive three-dimensional networks. Lectins, a class of glycoproteins containing glycosyl-specific recognition domains, play an important role in biological recognition. However, the fucose-specific lectins have rarely been studied regarding the process of preying on nematodes. In this study, we characterized the biological role of the fucose-specific lectin encoding gene AOL_s00054g276 (g276) in A. oligospora. The gene g276 was first deleted based on homologous recombination, then the phenotype and nematocidal activity of the Δg276 mutant was evaluated. The results showed that the deletion of gene g276 delayed trap formation and weakened its nematocidal activity; however, mycelial growth, conidia production, conidial germination rates, and adaption to environmental stresses were not affected. Our results suggest that the fucose-specific lectin encoding gene g276 might be associated with the morphogenesis of this fungus, and its deletion resulted in a significantly low density of three-dimensional traps (P < 0.05) and a significantly low nematode-tapping efficiency (P < 0.001). These findings provide a basis for further elucidating the mechanism of A. oligospora preying on nematodes and lay a foundation for the development and utilization of fungal-derived lectins for nematode control in future.

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The AL-ao379 gene plays a role in promoting the invasion stage of Bursaphelenchus xylophilus trapped by Arthrobotrys cladodes

Jia

Wang

et al. 2023

Preprint

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BACKGROUND: Nematode-trapping fungi can be used to develop specialized trapping devices to trap and kill nematodes, and the use of such fungi, with high efficiency and no toxicity to the environment, as biological control agents is very promising. Moreover, an understanding of the trap formation mechanism and the discovery of key pathogenic genes can help improve the efficacy of biocontrol agents. RESULTS: In this study, we used RNA-Seq to reveal the transcriptome characteristics of Arthrobotrys cladodes under Bursaphelenchus xylophilus induction. When many traps were observed to be produced, mycelia were collected and subjected to differential expression analysis. Differentially expressed genes were screened. AL-ao379 was identified by BLAST analysis and cloned by PCR. The results indicated that the AL-ao379 CDS was 1206 bp and encoded 402 amino acids. The expression of the AL-ao379 gene in different trapping stages was further compared by RT‒PCR. It was verified that the expression of the chitinase gene AL-ao379 increased significantly with the approach of the invasion stage and then decreased after reaching the highest levels in the invasion and predigestion stage. CONCLUSION: The chitinase gene AO-379 has been shown to affect the trapping responses of A. oligospora in soils, but to our knowledge, the effect of the chitinase on arboreal nematode-trapping fungi has not been previously reported. Our results demonstrated that the AL-ao379 gene was a key gene and a potential control target involved in trapping and that it was significantly expressed in invasion stages. In addition, we proposed a model of infestation mechanism of B. xylophilus trapping by arboreal nematode-trapping fungi.

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Thioredoxin1 regulates conidia formation, hyphal growth, and trap formation in the nematode-trapping fungus Arthrobotrys oligospora

Cited by 3 publications

References 21 publications

Methylglyoxal Has Different Impacts on the Fungistatic Roles of Ammonia and Benzaldehyde, and Lactoylglutathione Lyase Is Necessary for the Resistance of Arthrobotrys oligospora to Soil Fungistasis

Methylglyoxal Has Different Impacts on the Fungistatic Roles of Ammonia and Benzaldehyde, and Lactoylglutathione Lyase Is Necessary for the Resistance of Arthrobotrys oligospora to Soil Fungistasis

The fucose-specific lectin gene AOL_s00054g276 affects trap formation and nematocidal activity of the nematophagous fungus Arthrobotrys oligospora

The AL-ao379 gene plays a role in promoting the invasion stage of Bursaphelenchus xylophilus trapped by Arthrobotrys cladodes

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