Third‐generation recombinant immunoblot assay: comparison of reactivities according to hepatitis C virus genotype

Dow, B. C.; Munro, Hannah J.; Buchanan, Ian; Follett, E. A. C.; Davidson, Fiona; Yap, P. L.; Simmonds, Peter

doi:10.1046/j.1537-2995.1996.36696269515.x

Cited by 20 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…c HBsAg was not detected in these children when tested at 8 months of age. genotype 2 [Dow et al, 1996;Zein et al, 1997]. Data from this study suggest that infection with genotype 4 is highly prevalent in Tanzania, as in other African countries [Xu et al, 1994;Smuts and Kannemeyer, 1995;Oni and Harrison, 1996].…”

Section: Discussionmentioning

confidence: 54%

Prevalence and mother-to-infant transmission of hepatitis viruses B, C, and E in Southern Tanzania

et al. 1999

View full text Add to dashboard Cite

Hepatitis B and C markers were tested in 980 pregnant women, in the infants born to infected mothers, and in a random sample of 42 and 50, respectively, children born to uninfected mothers in Tanzania. Sixty-two women (6.3%) were positive for HBsAg and 15 (24%) were HBeAg-seropositive. Anti-HCV was detected in 49 women (5%), 15 (31%) of whom had detectable viremia. HCV RNA serum levels were low and only genotype 4 was identified. Sixty-six women (6.7%) were positive for anti-HIV, six of whom were coinfected with HBV and one with HCV. Anti-HEV was negative in the 180 women tested. At 8 months of age, HBsAg was detected in 8% and 2% of children born to HBV-infected and noninfected mothers, respectively (P = 0.2). Corresponding figures at 18 months of age were 31% and 21% (P = 0.3). When tested at 2 months of age, HCV RNA was not detected in any of the 43 children born to anti-HCV-positive mothers nor in any of 50 children born to anti-HCV-negative mothers. At 18 months, only one child, born to an anti-HCV-positive mother, had detectable HCV RNA. None of the infants born to women with HIV coinfection were infected with hepatitis viruses. This study suggests that exposure to HEV does not occur in southern Tanzania. The prevalence of current HBV infection in pregnant women from rural Tanzania is lower than in other sub-Saharan areas. In early childhood, HBV infection appears to occur by horizontal rather than maternofilial mechanisms of transmission. The prevalence of HCV infection is similar to that in other African countries. The results of this study show for the first time in Africa that mother-to-infant transmission does not play a significant role in the acquisition of HCV infection.

show abstract

Section: Discussionmentioning

confidence: 54%

Prevalence and mother-to-infant transmission of hepatitis viruses B, C, and E in Southern Tanzania

et al. 1999

View full text Add to dashboard Cite

show abstract

“…The occurrence of HCV RNA detectable in patients with negative anti-HCV can be a consequence of immunossupression with decreased production of antibodies, or the window period of a recent infection (Sharara et al 1996, Lok & Gunaratnam 1997. Another possibility is the absence, in the serology kits used, of an antigenic variant that is present in the geographic area studied (Dow et al 1996). Other studies also detected HCV or HBV nucleic acids in anti-HCV negative or HBsAg-negative/IgM anti-HBc-positive hemodialysis patients, respectively (Carneiro et al 2001, Lewis-Ximenez et al 2001.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis C and hepatitis B virus infection in different hemodialysis units in Belo Horizonte, Minas Gerais, Brazil

Busek

Baba

Filho

et al. 2002

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Consequently, it is difficult to identify the most sensitive assay to NS4. A recent report indicated that RIBA-3 lacked sensitivity to the NS4 antibody for HCV types 2 and 3 [9]. Not all the genotypes of the 44 positive samples of our panel were determined.…”

Section: Discussionmentioning

confidence: 93%

A Comparative Evaluation of the Sensitivity of Five Anti-Hepatitis C Virus Immunoblot Assays

et al. 1998

View full text Add to dashboard Cite

Background and Objectives: In order to compare sensitivity, five anti-hepatitis C virus (HCV) immunoblot assays were tested (Deciscan plus, Inno-Lia III, Matrix, Murex Western blot and RIBA-3). Materials and Methods: The test panel (50 samples for each assay) included 6 anti-HCV-negative samples and 44 samples from 36 HCV-infected subjects. Results: There were minor differences in core reactivity among the assays. The smallest number of NS3-reactive results occurred with the Murex and Matrix assays, and the smallest number of NS4 reactives with RIBA-3 and Matrix. Among the 20 discrepant results for NS5 there was one clear false-negative with Inno-Lia. Only 28 of the 50 samples of the panel gave the same results in all the assays: 5 negatives and 23 positives. One of the 6 negative samples was indeterminate in 3 assays. Eighteen of the 21 other divergent results were interpreted as either indeterminate or positive, a common reactivity being exhibited by all 5 assays. The most important discrepancies occurred on 3 HCV-RNA-positive samples which came up negative in some assays: 2 samples with isolated NS3 reactivity were negative by Matrix and Murex Western blot, 1 of them being also negative by Inno-Lia III; another sample was negative by RIBA-3 and Matrix due to weak signals (< 1) on core and NS3 proteins, which did not exceed 1+ with the other assays. Conclusions: With more uniform criteria for interpretation, the results would have been less divergent. Some assays should improve their sensitivity to the NS3 protein.

show abstract

Third‐generation recombinant immunoblot assay: comparison of reactivities according to hepatitis C virus genotype

Abstract: The current RIBA-3 lacks sensitivity to the NS4 antibody for HCV types 2 and 3. The incorporation of type-specific components to other genotypes for NS4 (and NS3) antigens should be considered by the manufacturers.

Cited by 20 publications

References 21 publications

Prevalence and mother-to-infant transmission of hepatitis viruses B, C, and E in Southern Tanzania

Prevalence and mother-to-infant transmission of hepatitis viruses B, C, and E in Southern Tanzania

Hepatitis C and hepatitis B virus infection in different hemodialysis units in Belo Horizonte, Minas Gerais, Brazil

A Comparative Evaluation of the Sensitivity of Five Anti-Hepatitis C Virus Immunoblot Assays

Contact Info

Product

Resources

About