Third Type of Plasmid Conferring Gentamicin Resistance in
            <i>Pseudomonas aeruginosa</i>

Smith, Jeremy C.; Lus, R. Gömez; Calvo, Miguel; Datta, Naomi; Jacob, A. E.; Hedges, R. W.

doi:10.1128/aac.8.3.227

Cited by 30 publications

(9 citation statements)

References 18 publications

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“…Tn5036 is from an E. cloacae strain isolated from the intestine of a toad found near a mercury mine in Russia and the closely related Tn3926 is from a Y. enterocolitica strain found in milk in France. R1033, which contains Tn1696, was recovered from a clinical Pseudomonas strain isolated in Spain prior to 1975 (42). This emphasizes the connection between environmental, food-borne, and clinical bacteria.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transposons Tn 1696 and Tn 21 and Their Integrons In4 and In2 Have Independent Origins

Partridge

Brown

Stokes

et al. 2001

Antimicrob Agents Chemother

169

160

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The first 13.6 kb of the mercury and multidrug resistance transposon Tn1696, which includes the class 1 integron In4, has been sequenced. In4 is 8.33 kb long and contains the 5-conserved segment (5-CS) and 2.24 kb of the 3-conserved segment (3-CS) flanking four integrated cassettes. The 3-CS region is followed by one full copy and an adjacent partial copy of the insertion sequence IS6100 flanked, in inverse orientation, by two short segments (123 and 152 bp) from the outer right-hand end of class 1 integrons. This structure is representative of a distinct group of class 1 integrons that differs from In2, found in Tn21, and other related class 1 integrons. In4 does not include transposition genes but is bounded by characteristic 25-bp inverted repeats and flanked by a direct duplication of 5 bp of the target sequence, indicating that it was inserted by a transpositional mechanism. In4 lies between the resII and resI sites of a backbone mercury resistance transposon which is >99.5% identical to Tn5036. Although Tn21 and Tn1696 are both classified as members of the Tn21 subfamily of the Tn3 transposon family, the backbone mercury resistance transposons are only 79 to 96% identical. Tn21 also contains a region of about 0.7 kb not found in Tn1696. The integrons In2 and In4 carrying the antibiotic resistance genes have been inserted at different locations into distinct ancestral mercury resistance transposons. Thus, Tn21 and Tn1696 have independent histories and origins. Other transposons (Tn1403 and Tn1412) that include a class 1 integron also have independent origins. In all except Tn21, the integron is located within the res region of the backbone transposon.

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Section: Discussionmentioning

confidence: 99%

“…Tn1696 was originally found in plasmid R1033 (36), isolated from a Pseudomonas aeruginosa strain (42), and Tn21 was found in plasmid NR1 (R100) (10), isolated from a Shigella flexneri strain (29). Early comparisons revealed similarities (40).…”

mentioning

confidence: 99%

Transposons Tn 1696 and Tn 21 and Their Integrons In4 and In2 Have Independent Origins

Partridge

Brown

Stokes

et al. 2001

Antimicrob Agents Chemother

169

160

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“…Plasmids RP4, R702 or R1033, for example, interact reciprocally with R751, eliminating and being eliminated by it Smith et al, 1975). Plasmid R772 eliminates R751 efficiently but is subject to elimination only after a long delay and, in the absence of selection, strains carrying both plasmids segregate far more R75 1-progeny than R772-progeny.…”

Section: Discussionmentioning

confidence: 99%

Properties of R Plasmid R772 and the Corresponding Pilus-specific Phage PR772

Coetzee

Lecatsas

Coetzee

et al. 1979

Journal of General Microbiology

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R plasmid R772 was isolated from a strain of Proteus mirabilis and is a self-transmissible P-1 incompatibility group plasmid having a molecular weight of about 27 x lo6. It renders bacterial hosts resistant to kanamycin. Phage PR772 was isolated as a phage dependent on the presence of R772 in bacterial hosts. It is hexagonal-shaped with a diameter of 53 nm, has a thick inner membrane and no tail. Vaguely defined appendages are sometimes apparent at some vertices and the phage possesses double-stranded DNA. The DNA has a guanine plus cytosine molar content of 48':d. The phage is sensitive to chloroform and has a buoyant density of 1.26 g ~m -~. These observations suggested that the inner membrane of the phage could contain lipid. Phage PR772 differs in morphology from the doublestranded DNA plasmid-specific phages PR4 and PRRl which adsorb to tips and sides, respectively, of sex pili coded for by P-1 incompatibility group plasmids. Phage PR772 formed clear plaques which varied in diameter. Serologically, phages PR772 and PR4 are possibly related though very distantly, but the two phages have identical host ranges. Phage PR772 adsorbed by one of its apices to tips of sex pili coded for by plasmid R772 in Escherichia coli. It also formed plaques on Salmonella typhimurium, Proteus morganii and Providence strains harbouring this plasmid as well as strains of E. coli carrying plasmids of incompatibility groups N or W. The phage produced areas of partial clearing on lawns of P. mirabilis PM5006 harbouring plasmid R772, the P-1 incompatibility group plasmid RP4, the W group plasmid RSa or the N group plasmid N3, and on lawns of Providence strain P29 carrying plasmid RP4.

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“…In4 was found in plasmid R1033 of a P. aeruginosa strain isolated in 1975 (23). The In151 backbone differed from that of In4 by the presence of a partial copy of IS6100; i.e., In151 had the 5=-CS, 3=-CS, and a complete copy of IS6100 located downstream of the 3=-CS.…”

Section: Aac(6=)-iaj In the Class 1 Integronmentioning

confidence: 99%

Novel 6′- N -Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa

Tada

Miyoshi‐Akiyama

Shimada

et al. 2013

Antimicrob Agents Chemother

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bPseudomonas aeruginosa NCGM1588 has a novel chromosomal class 1 integron, In151, which includes the aac(6=)-Iaj gene. The encoded protein, AAC(6=)-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6=)-Ia. Escherichia coli transformed with a plasmid containing the aac(6=)-Iaj gene acquired resistance to all aminoglycosides tested except gentamicin. Of note, aac(6=)-Iaj contributed to the resistance to arbekacin. Thin-layer chromatography revealed that AAC(6=)-Iaj acetylated all aminoglycosides tested except gentamicin. These findings indicated that AAC(6=)-Iaj is a functional acetyltransferase that modifies the amino groups at the 6= positions of aminoglycosides and contributes to aminoglycoside resistance of P. aeruginosa NCGM1588, including arbekacin.

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Third Type of Plasmid Conferring Gentamicin Resistance in Pseudomonas aeruginosa

Cited by 30 publications

References 18 publications

Transposons Tn 1696 and Tn 21 and Their Integrons In4 and In2 Have Independent Origins

Transposons Tn 1696 and Tn 21 and Their Integrons In4 and In2 Have Independent Origins

Properties of R Plasmid R772 and the Corresponding Pilus-specific Phage PR772

Novel 6′- N -Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa

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