Thoracic Aortas from Multiorgan Donors Are Suitable for Obtaining Resident Angiogenic Mesenchymal Stromal Cells

Pasquinelli, Gianandrea; Tazzari, Pier Luigi; Vaselli, Cristiana; Foroni, Laura; Buzzi, Marina; Storci, Gianluca; Alviano, Francesco; Ricci, Francesca; Bonafè, Massimiliano; Orrico, Catia; Bagnara, Gian Paolo; Stella, Andréa; Conte, Roberto

doi:10.1634/stemcells.2006-0731

Cited by 121 publications

(118 citation statements)

References 34 publications

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“…2 As discussed in the following sections, these consist of lineage-committed endothelial progenitor cells (EPCs) 4 and smooth muscle progenitor cells (SPCs), 5,6 multipotent vascular stem cells (MVSCs), 7 mesenchymal stem/stromal cells (MSCs), 8,9 adventitial macrophage progenitor cells (AMPCs), and circulation-derived HSCs. 10,11 …”

Section: Vw-pcs Vascular Wall Progenitor Cellsmentioning

confidence: 99%

Vascular Wall Progenitor Cells in Health and Disease

Psaltis

Simari

2015

Circulation Research

173

155

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The vasculature plays an indispensible role in organ development and maintenance of tissue homeostasis, such that disturbances to it impact greatly on developmental and postnatal health. Although cell turnover in healthy blood vessels is low, it increases considerably under pathological conditions. The principle sources for this phenomenon have long been considered to be the recruitment of cells from the peripheral circulation and the re-entry of mature cells in the vessel wall back into cell cycle. However, recent discoveries have also uncovered the presence of a range of multipotent and lineage-restricted progenitor cells in the mural layers of postnatal blood vessels, possessing high proliferative capacity and potential to generate endothelial, smooth muscle, hematopoietic or mesenchymal cell progeny. In particular, the tunica adventitia has emerged as a progenitor-rich compartment with niche-like characteristics that support and regulate vascular wall progenitor cells. Preliminary data indicate the involvement of some of these vascular wall progenitor cells in vascular disease states, adding weight to the notion that the adventitia is integral to vascular wall pathogenesis, and raising potential implications for clinical therapies. This review discusses the current body of evidence for the existence of vascular wall progenitor cell subpopulations from development to adulthood and addresses the gains made and significant challenges that lie ahead in trying to accurately delineate their identities, origins, regulatory pathways, and relevance to normal vascular structure and function, as well as disease.

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Section: Vw-pcs Vascular Wall Progenitor Cellsmentioning

confidence: 99%

Vascular Wall Progenitor Cells in Health and Disease

Psaltis

Simari

2015

Circulation Research

173

155

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“…Adventitial cells participate in vascular growth and repair and are important determinants of lumen size via control of inward or outward wall remodeling processes (2,5). Recent studies report unexpected roles for the adventitia insofar as it supports resident progenitor cells that can adopt both vascular and nonvascular fates (6)(7)(8). Despite these important functional properties, the signals and environmental cues that confer such unique and dynamic properties to the adventitial layer remain largely unknown.…”

mentioning

confidence: 99%

“…The adventitia responds to balloon catheter injury with rapid increases in SM␣Actin and SM22␣ expression, particularly at the media-adventitia interface (5). Endothelial progenitor cells are reported to cluster between the media and adventitia in human arteries (7,8). These cells formed capillary sprouts in ex vivo ring culture assays and were recruited by tumor cells for capillary vessel formation in vivo.…”

mentioning

confidence: 99%

A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1 ⁺ smooth muscle progenitor cells

Passman

Dong²,

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

272

342

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We characterize a sonic hedgehog (Shh) signaling domain restricted to the adventitial layer of artery wall that supports resident Sca1-positive vascular progenitor cells (AdvSca1). Using patched-1 (Ptc1 lacZ ) and patched-2 (Ptc2 lacZ ) reporter mice, adventitial Shh signaling activity was first detected at embryonic day (E) 15.5, reached the highest levels between postnatal day 1 (P1) and P10, was diminished in adult vessels, and colocalized with a circumferential ring of Shh protein deposited between the media and adventitia. In Shh ؊/؊ mice, AdvSca1 cells normally found at the aortic root were either absent or greatly diminished in number. Using a Wnt1-cre lineage marker that identifies cells of neural crest origin, we found that neither the adventitia nor AdvSca1 cells were labeled in arteries composed of neural crest-derived smooth muscle cells (SMCs). Although AdvSca1 cells do not express SMC marker proteins in vivo, they do express transcription factors thought to be required for SMC differentiation, including serum response factor (SRF) and myocardin family members, and readily differentiate to SMC-like cells in vitro. However, AdvSca1 cells also express potent repressors of SRF-dependent transcription, including Klf4, Msx1, and FoxO4, which may be critical for maintenance of the SMC progenitor phenotype of AdvSca1 cells in vivo. We conclude that a restricted domain of Shh signaling is localized to the arterial adventitia and may play important roles in maintenance of resident vascular SMC progenitor cells in the artery wall.artery ͉ differentiation ͉ Klf4 ͉ self-renewal ͉ serum response factor

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“…Early studies demonstrated that MSCs formed capillary-like structures, composed of an endothelial tube covered by PCs. 40,41 Most MSCs prepared in these studies are composed of heterogenous cell populations that may contain endothelial-and PC-progenitor cells or pluripotent stem cells. 42 In this study, clonal mPCs acquired both endothelial-and PC-like phenotypes in the presence of VEGF and formed capillary-like structure on their own in Matrigel ( Figure 6).…”

Section: Mpcs Are Capillary-forming Stem Cellsmentioning

confidence: 99%

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

Kabara

Kawabe

Matsuki

et al. 2014

Laboratory Investigation

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Adventitial microvessels, vasa vasorum in the vessel walls, have an active role in the vascular remodeling, although its mechanisms are still unclear. It has been reported that microvascular pericytes (PCs) possess mesenchymal plasticity. Therefore, microvessels would serve as a systemic reservoir of stem cells and contribute to the tissues remodeling. However, most aspects of the biology of multipotent PCs (mPCs), in particular of pathological microvessels are still obscure because of the lack of appropriate methods to detect and isolate these cells. In order to examine the characteristics of mPCs, we established immortalized cells residing in adventitial capillary growing at the injured vascular walls. We recently developed in vivo angiogenesis to observe adventitial microvessels using collagen-coated tube (CCT), which also can be used as an adventitial microvessel-rich tissue. By using the CCT, CD146-or NG2-positive cells were isolated from the adventitial microvessels in the injured arteries of mice harboring a temperature-sensitive SV40 T-antigen gene. Several capillary-derived endothelial cells (cECs) and PCs (cPCs) cell lines were established. cECs and cPCs maintain a number of key endothelial and PC features. Co-incubation of cPCs with cECs formed capillary-like structure in Matrigel. Three out of six cPC lines, termed capillary mPCs demonstrated both mesenchymal stem cell-and neuronal stem cell-like phenotypes, differentiating effectively into adipocytes, osteoblasts, as well as schwann cells. mPCs differentiated to ECs and PCs, and formed capillary-like structure on their own. Transplanted DsRed-expressing mPCs were resident in the capillary and muscle fibers and promoted angiogenesis and myogenesis in damaged skeletal muscle. Adventitial mPCs possess transdifferentiation potential with unique phenotypes, including the reconstitution of capillary-like structures. Their phenotype would contribute to the pathological angiogenesis associated with vascular remodeling. These cell lines also provide a reproducible cellular tool for high-throughput studies on angiogenesis, vascular remodeling, and regeneration as well.

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Thoracic Aortas from Multiorgan Donors Are Suitable for Obtaining Resident Angiogenic Mesenchymal Stromal Cells

Abstract: ABSTRACT

Cited by 121 publications

References 34 publications

Vascular Wall Progenitor Cells in Health and Disease

Vascular Wall Progenitor Cells in Health and Disease

A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1 ⁺ smooth muscle progenitor cells

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

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Thoracic Aortas from Multiorgan Donors Are Suitable for Obtaining Resident Angiogenic Mesenchymal Stromal Cells

Abstract: ABSTRACT

Cited by 121 publications

References 34 publications

Vascular Wall Progenitor Cells in Health and Disease

Vascular Wall Progenitor Cells in Health and Disease

A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1 + smooth muscle progenitor cells

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

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A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1 ⁺ smooth muscle progenitor cells