THP-1 cells as a model for human monocytes

Bosshart, Herbert; Heinzelmann, Michael

doi:10.21037/atm.2016.08.53

Cited by 242 publications

(190 citation statements)

References 15 publications

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“…Even though our approach show differential responses of THP-1 and primary human macrophages, as shown elsewhere, cells (Bosshart and Heinzelmann, 2016, Heil, et al, 2002) CD163 consistently induced an anti-inflammatory phenotype if both types of cells. We believe that inducing CD163 could not only be beneficial for promoting resolution of inflammation and treating related pain conditions, but also protect from bacterial infections.…”

Section: Discussionsupporting

confidence: 74%

“…The clinical relevance of our approach lies in the fact that in our studies we use human primary macrophages (Bosshart and Heinzelmann, 2016, Heil, et al, 2002), demonstrating that our target, CD163, retains its anti-inflammatory functions in clinically relevant human cells under potent (LPS) inflammatory conditions. Even though our approach show differential responses of THP-1 and primary human macrophages, as shown elsewhere, cells (Bosshart and Heinzelmann, 2016, Heil, et al, 2002) CD163 consistently induced an anti-inflammatory phenotype if both types of cells.…”

Section: Discussionmentioning

confidence: 99%

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Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions

et al. 2017

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M1 macrophages release pro-inflammatory factors during inflammation. They transition to an M2 phenotype and release anti-inflammatory factors to resolve inflammation. An imbalance in the transition from M1 to M2 phenotype in macrophages contributes to the development of persistent inflammation. CD163, a member of the scavenger receptor cysteine-rich family, is an M2 macrophage marker. The functional role of CD163 during the resolution of inflammation is not completely known. We postulate that CD163 contributes to the transition from M1 to M2 phenotype in macrophages. We induced CD163 gene in THP-1 and primary human macrophages using polyethylenimine nanoparticles grafted with a mannose ligand (Man-PEI). This nanoparticle specifically targets cells of monocytic origin via mannose receptors. Cells were challenged with a single or a double stimulation of lipopolysaccharide (LPS). A CD163 or empty plasmid was complexed with Man-PEI nanoparticles for cell transfections. Quantitative RT-PCR, immunocytochemistry, and ELISAs were used for molecular assessments. CD163-overexpressing macrophages displayed reduced levels of tumor necrosis factor-alpha (TNF)-α and monocytes chemoattractant protein (MCP)-1 after a single stimulation with LPS. Following a double stimulation paradigm, CD163-overexpressing macrophages showed an increase of interleukin (IL)-10 and IL-1ra, and a reduction of MCP-1. This anti-inflammatory phenotype was partially blocked by an anti-CD163 antibody (effects on IL-10 and IL-1ra). A decrease in the release of TNF-α, IL-1β, and IL-6 was observed in CD163-overexpressing human primary macrophages. The release of IL-6 was blocked by an anti-CD163 antibody in the CD163-overexpressing group. Our data show that the induction of the CD163 gene in human macrophages under inflammatory conditions produces changes in cytokine secretion in favor of an anti-inflammatory phenotype. Targeting macrophages to induce CD163 using cell-directed nanotechnology is an attractive and practical approach for inflammatory conditions that could lead to persistent pain, i.e. major surgeries, burns, rheumatoid arthritis, etc.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions

et al. 2017

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“…In 2016, Bosshart and Heinzelmann concluded that the THP‐1 monocytes line was not reliable to investigate LPS effects. Indeed, primary cultures are more relevant than cell lines, retaining most of their markers and functions observed in vivo (Bosshart & Heinzelmann, ; Pan, Kumar, Bohl, Klingmueller, & Mann, ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Porphyromonas gingivalis LPS and LR12 peptide on TREM‐1 expression by monocytes

Dubar

Carrasco²,

Gibot

et al. 2018

J Clinic Periodontology

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“…In order to study the possible anti‐inflammatory effect of our cyto‐protective lead compounds, we deliberated the Lipo polysaccharide ( LPS ) induced inflammatory response on THP‐1 cells as a model for human monocytes . In an inflammatory response, cytokines (inter leukin, IL‐6, TNF alpha) are produced and their concentration in the medium can be measured using ELISA technique.…”

Section: Resultsmentioning

confidence: 99%

Cyanuric‐Chloride‐Mediated Synthesis of 2‐Aryl‐3‐tert‐butoxycarbonyl‐thiazolidine‐4‐carboxylic Acid Anilides: Mechanistic, X‐Ray Crystal Structures and Cytotoxicity Studies

et al. 2019

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The ‘2R,4R’‐2‐aryl thiazolidine‐4‐carboxylic acid anilides (ATCAAs) were synthesized using cyanuric chloride (CC) as a clean and substoichiometric acid‐amine coupling agent under optimized reaction conditions. The diesterphenol intermediate based mechanism is proposed and supported by spectral characterization of the intermediate. The single crystal X‐ray structures of acid substrate (2R,4R)‐3‐(tert‐butoxycarbonyl)‐2‐(2‐methoxyphenyl)thiazolidine‐4‐carboxylic acid (3 a) and anilides (R)‐tert‐butyl 4‐(2‐fluorophenylcarbamoyl)thiazolidine‐3‐carboxylate (4 b), (2R,4R)‐tert‐butyl 2‐(2,6‐dichlorophenyl)‐4‐(m‐tolylcarbamoyl)thiazolidine‐3‐carboxylate (6 b) confirmed the formation of chiral anilides. The synthesized library of anilides [(R)‐tert‐butyl 4‐Aryl thiazolidine‐3‐carboxylate and (2R,4R)‐tert‐butyl 2‐Aryl‐4‐Aryl thiazolidine‐3‐carboxylate] 4 a‐6 f was screened for their in vitro anti cancer, neuronal and neuroprotective studies. The anilides 4 b, 4 g, 5 d, 5 h, 6 c and 6 f (where the aryl=2‐fluorophenylcarbamoyl, 4‐chlorophenylcarbamoyl, 2‐methoxyphenyl, 2,5‐dichlorophenylcarbamoyl, 2,6‐dichlorophenyl, 4‐chlorophenylcarbamoyl respectively)were found to be less cytotoxic towards N2 A, SHSY‐5Y neuronal cell lines in their differentiated and undifferentiated forms, and also exhibited dose dependant anti‐inflammatory properties for a possible identification towards neurodegenerative and inflammatory disorders.

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THP-1 cells as a model for human monocytes

Cited by 242 publications

References 15 publications

Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions

Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions

Effects of Porphyromonas gingivalis LPS and LR12 peptide on TREM‐1 expression by monocytes

Cyanuric‐Chloride‐Mediated Synthesis of 2‐Aryl‐3‐tert‐butoxycarbonyl‐thiazolidine‐4‐carboxylic Acid Anilides: Mechanistic, X‐Ray Crystal Structures and Cytotoxicity Studies

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