Three-dimensional chitosan scaffold-based MCF-7 cell culture for the determination of the cytotoxicity of tamoxifen

Dhiman, Harpreet Kaur; Ray, Alok R.; Panda, Amulya K.

doi:10.1016/j.biomaterials.2004.04.012

Cited by 187 publications

(142 citation statements)

References 26 publications

Supporting

Mentioning

134

Contrasting

Unclassified

Order By: Relevance

“…Prior research has suggested that the efficacy of potential anticancer drugs in vivo differs when compared with in vitro studies using cancer cells grown in monolayer (31,44). Because three-dimensional culture of cancer cell lines has long been advocated as a better model of the malignant phenotype in vivo (31, 45), we evaluated the effect of SD-1029 on chordoma cell lines in three-dimensional culture.…”

Section: Discussionmentioning

confidence: 99%

A novel target for treatment of chordoma: signal transducers and activators of transcription 3

Yang

Schwab

Schoenfeld

et al. 2009

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

A major obstacle in the effective treatment of chordoma is that there are no identifiable biomarkers capable of predicting prognosis. Recent research has indicated that signal transducers and activators of transcription (Stat3) may be an important prognostic marker in some cancers, but its role in chordoma tumors has not been elucidated. In this study, the expression of Stat3 was evaluated in chordoma tissue microarray that contains 70 chordoma samples. Cells in the tissue microarray showed nuclear staining for phosphorylated Stat3 in all instances. The level of phosphorylated Stat3 expression correlated with the survival and severity of the disease. Three chordoma cell lines were exposed to SD-1029, a novel inhibitor of Stat3 activation. MTT assay showed that the growth of all chordoma cell lines was inhibited by SD-1029. The expression of Stat3 signaling cascade was inhibited in all chordoma cell lines after treatment with SD-1029. The cytotoxicity of the combination of SD-1029 and chemotherapeutic drugs is significantly better than either agent alone. Phosphorylation of Stat3 in chordoma cells in vitro and cellular proliferation in three-dimensional culture were inhibited by SD-1029. In conclusion, the Stat3 pathway is constitutively activated in chordomas and the level of expression may serve as a predictor for prognosis. Blockade of the Stat3 pathway represents a potential strategy for future treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

A novel target for treatment of chordoma: signal transducers and activators of transcription 3

Yang

Schwab

Schoenfeld

et al. 2009

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

show abstract

“…Poly(lactidecoglycolide), poly(lactic acid), poly(vinyl alcohol), poly( -caprolactone) or polyethylene glycol) (e.g. Poly(lactide-coglycolide), poly(lactic acid), poly(vinyl alcohol), poly( -caprolactone) or polyethylene glycol) [77,78,99,[115][116][117][118][119][120][121]. One advantage of scaffold-based strategies is that it is possible to better manage matrix stiffness/compliance, porosity, and biochemical composition.…”

Section: Mimicking the Ecmmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

View full text Add to dashboard Cite

A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

show abstract

“…Because 3-D culture of MCF-7 cells on chitosan matrices provided a better understanding of the cell's carbohydrate metabolism, Panda and coworkers further studied the cytotoxic effect of anticancer drug and, more importantly, the kinetics and uptake of cathepsin D in breast cancer cells (Dhiman et al 2005). On chitosan scaffolds, the MCF-7 cells produced more lactic acid in an amount similar to that observed for tumor cells in vivo, suggesting that their metabolism more closely resembled that observed in tissues.…”

Section: Chitosanmentioning

confidence: 99%

Three-dimensional polymeric systems for cancer cell studies

Burg

2007

Cytotechnology

View full text Add to dashboard Cite

Three-dimensional (3-D) culture of cancer cells and of normal mammalian cells in a polymeric matrix is generally a better alternate model for understanding the regulation of cancer cell proliferation and for evaluation of different anticancer drugs. A substantial amount of evidence demonstrates important differences in the behavior of cells grown in monolayer, i.e., two-dimensional (2-D), and in 3-D cultures. Cancer cells grown in 3-D culture are more resistant to cytotoxic agents than cells in 2-D culture; growth of cells in vitro in 3-D requires a suitable polymer that provides a structural scaffold for cell adhesion and growth. Many naturally derived polymers as well as synthetic polymers have been investigated as scaffolds. The aim of this review is to overview the polymeric materials of natural and synthetic origin that are of specific interest to 3-D cell cultures, and discuss the development of new polymers that should be specifically designed for 3-D culture applications.

show abstract

Three-dimensional chitosan scaffold-based MCF-7 cell culture for the determination of the cytotoxicity of tamoxifen

Cited by 187 publications

References 26 publications

A novel target for treatment of chordoma: signal transducers and activators of transcription 3

A novel target for treatment of chordoma: signal transducers and activators of transcription 3

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Three-dimensional polymeric systems for cancer cell studies

Contact Info

Product

Resources

About