Three-dimensional Micro-culture System for Tooth Tissue Engineering

Abstract

The arrangement of cells within a tissue plays an essential role in organogenesis, including tooth development. Progress is being made to regenerate teeth by reassociating dissociated embryonic dental cells and implanting them in vivo. In the present study, we tested the hanging drop method to study mixed epithelial-mesenchymal cell reorganization in a liquid instead of semisolid medium to see whether it could lead to tooth histogenesis and organogenesis. This method allowed the control of the proportion and number of cells to be used, and the forming microtissues showed homogeneous size. The liquid environment favored cell migrations as compared with collagen gels. Three protocols were compared. The one that sequentially combined the hanging drop and semisolid medium cultures prior to in vivo implantation gave the best results. Indeed, after implantation, teeth developed, showing a well-formed crown, mineralization of dentin and enamel, and the initiation of root formation. Vascularization and the cellular heterogeneity in the mesenchyme were similar to what was observed in developing molars. Finally, after coimplantation with a trigeminal ganglion, the dental mesenchyme, including the odontoblast layer, became innervated. The real advantage of this technique is the small number of cells required to make a tooth. This experimental model can be employed to study the development, physiology, metabolism, or toxicology in forming teeth and test other cell sources.

Semaphorin 3A receptor inhibitor as a novel therapeutic to promote innervation of bioengineered teeth

“…Tissue engineering is a vast field aiming to fully regenerate functional tissues and organs (Langer & Vacanti, ). Different models for tooth engineering were proposed using reassociations of dental mesenchymal and epithelial cells (Honda, Tsuchiya, Sumita, Sagara, & Ueda, ; Kökten et al, ; Kökten, Lesot, & Kuchler‐Bopp, ; Kuchler‐Bopp et al, ; Nait Lechguer et al, ; Nait Lechguer, Kuchler‐Bopp, Hu, Haïkel, & Lesot, ; Nakao et al, ; Oshima et al, ) or using stem cells (Ohazama, Modino, Miletich, & Sharpe, ; Volponi, Pang, & Sharpe, ). In mouse, the first trigeminal axons reach the mandibular first molar tooth germ region at the bud stage at embryonic day‐13 (E13; Kettunen et al, ).…”

Elaboración de un biodiente: enfoque actual y desafíos

“…Finalmente, y para propiciar la inervación del mesénquima dental, incluyendo la capa odontoblástica, se realizó un coimplante en el ganglio del trigémino. Este modelo puede reproducir las interacciones epitelio mesénquima que permiten un desarrollo óptimo de los dientes y una ventaja es el pequeño número de células que se requiere (50).…”

“…Conseguir una adecuada vascularización es uno de los grandes desafíos asociados a la consecución de un diente de bioingeniería tanto en modelos in vitro como in vivo, sobre lo cual se debe seguir trabajando (5,47), aunque los avances en este sentido han sido satisfactorios con algunos modelos de bioingeniería dental (7,38,50,57).…”

Dental application of the induced pluripotent stem cells in stomatology

“…Как известно, полнофункциональный зуб может быть сформирован из диссоциированных клеток зародыша зуба. Несколько групп исследователей показали, что можно получить биологический зуб, сходный по внешнему виду с натуральным, в результате рекомбинации типа ткань-клетка или клетка-клетка с использованием клеток эмбрионального зародыша зуба [53][54][55]. Кроме того, классические рекомбинационные исследования показали, что в зависимости от эмбриональной стадии, сначала зубной эпителий, а затем зубная мезенхима имеют одонтогенный индуктивный потенциал [56].…”