Three-Dimensional Reconstruction of a Dictyosome using Serial Sections

Kristen, U.; Lockhausen, J.; Robinson, David G.

doi:10.1093/jxb/35.8.1113

Cited by 11 publications

(4 citation statements)

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“…Intercisternal membrane connections or associations of ER cisternae with dictyosomes were not taken into account in the previous models. The first true-scale reconstruction of a dictyosome, however, indicated connections between adjacent Golgi cisternae and between the dictyosomal stack and the ER (Kristen et al, 1984). The latter detail supported the suggestion that, even in higher plant cells, the dictyosomes are inserted densely into the meshwork of the endomembrane system.…”

Section: Discussionmentioning

confidence: 61%

Three‐dimensional reconstruction of a plant dictyosome from series of ultrathin sections using computer image processing

Lockhausen¹,

Kristen²,

Menhardt

et al. 1990

Journal of Microscopy

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SUMMARY A single dictyosome from an actively secreting ovary gland cell of Aptenia cordifolia has been reconstructed in 3‐D from a series of twenty‐nine electron micrographs by computer image processing. The reconstruction is presented under different viewing angles in the form of shaded perspective displays. From these displays the entire dictyosome, surrounded by numerous vesicles, appears to be more a spherical than a flat body. The plate‐like region of the dictyósome is demonstrated when only a portion of the electron micrographs is used for the image processing, leading to ‘cut‐off’ displays. Since some upper planes were removed, such ‘cut‐off’ displays revealed both tubular connections between cisternae of the dictyosome and the neighbouring endoplasmic reticulum as well as tubular continuities between adjacent Golgi cisternae within the same stack. Possible consequences of both types of interconnections on transport and processing of proteins and glycoproteins are discussed.

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Section: Discussionmentioning

confidence: 61%

Three‐dimensional reconstruction of a plant dictyosome from series of ultrathin sections using computer image processing

Lockhausen¹,

Kristen²,

Menhardt

et al. 1990

Journal of Microscopy

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“…Robinson and Kristen (1982) showed a general diagrammatic representation of Golgi apparatus stack polarization based on ultrastructural and cytochemical findings in plant cell dictyosomes. More recently, Kristen et al (1984) have reported a three-dimensional pattern of dictyosome morphology. Our results do not bring additional information to this point but provide numerical data related to the position of every element in the stack.…”

Section: Discussionmentioning

confidence: 99%

“…Potassium permanganate fixation has been largely used since its strong oxidative nature allows a rapid penetration and fixation of the tissues (Kristen et al, 1984). By applying this technique, several processes related to cytokinesis in plant cells were early described (Frey-Wyssling et al, 1964;Whaley and Mollenhauer, 1963).…”

Section: Introductionmentioning

confidence: 99%

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Differential morphometric values induced in Golgi apparatus of higher plant cells by aldehyde and permanganate fixation

González‐Reyes

Gracia‐Navarro

Garcı́a-Herdugo

et al. 1989

J. Elec. Microsc. Tech.

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In order to determine the best conditions to carry out quantitative ultrastructural studies in plant specimens, five different fixation techniques, including some of the most reported electron microscopy fixatives (glutaraldehyde-paraformaldehyde, osmium tetroxide, potassium permanganate), were assayed in onion root meristems to check their ability to induce morphometric changes in Golgi apparatus ultrastructure. Although the parameters evaluated showed in all cases the same tendency, values obtained after permanganate fixation were always higher than those found after aldehyde techniques (especially aldehyde-osmium). Aldehyde followed by osmium fixation appears as the most indicated fixation method when accurate quantitative ultrastructural studies are to be developed.

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