Three-dimensional surface reconstruction and in situ site-specific cutting of the teliospores of Puccinia miscanthi causing leaf rust of the biomass plant Miscanthus sinensis

Kim, Ki Woo

doi:10.1016/j.micron.2015.03.007

Cited by 6 publications

(8 citation statements)

References 17 publications

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“…The scanning electron microscope (SEM) is commonly employed to observe biological objects with various dimensions, because of its large depth-of-focus, broad magnification range, and high spatial resolution down to 1 nm (Schroettner et al, 2006). Due to the inherent operating mechanisms of SEM, three-dimensional (3D) structures of biological objects in nature are projected onto a two-dimensional (2D) image plane in SEM (Kim, 2015). It is therefore impossible to take direct measurements of conventional morphological parameters such as the height and depth of 3D structures by conventional SEM (Kim, 2015).…”

Section: Beyond 2d Scanning Electron Microscopesmentioning

confidence: 99%

Biomedical Applications of Stereoscopy for Three-Dimensional Surface Reconstruction in Scanning Electron Microscopes

Kim

2016

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The scanning electron microscope (SEM) offers two-dimensional (2D) micrographs of three-dimensional (3D) objects due to its inherent operating mechanisms. To overcome this limitation, other devices have been used for quantitative morphological analysis. Many efforts have been made on the applications of software-based approaches to 3D reconstruction and measurements by SEM. Based on the acquisition of two stereo images, a multi-view technique consists of two parts: (i) geometric calibration and (ii) image matching. Quantitative morphological parameters such as height and depth could be nondestructively measured by SEM combined with special software programs. It is also possible to obtain conventional surface parameters such as roughness and volume of biomedical specimens through 3D SEM surface reconstruction. There is growing evidence that conventional 2D SEM without special electron detectors can be transformed to 3D SEM for quantitative measurements in biomedical research.

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Section: Beyond 2d Scanning Electron Microscopesmentioning

confidence: 99%

Biomedical Applications of Stereoscopy for Three-Dimensional Surface Reconstruction in Scanning Electron Microscopes

Kim

2016

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“…M. sinensis leaf rust occurred in Korea, where the causal agent was identified as the basidiomycetous fungus Puccinia miscanthi Miura ( Hiratsuka, 1958 ; Lee, 2001 ). While the aeciospores are found on Plantago species ( Hiratsuka, 1958 ), urediniospores and teliospores are present on M. sinensis , rendering the rust fungus heteroecious ( Kim, 2015 ). A recent study ( Kim, 2015 ) revealed the surface details and in situ cutting of P. miscanthi teliospores by FESEM.…”

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confidence: 99%

“…While the aeciospores are found on Plantago species ( Hiratsuka, 1958 ), urediniospores and teliospores are present on M. sinensis , rendering the rust fungus heteroecious ( Kim, 2015 ). A recent study ( Kim, 2015 ) revealed the surface details and in situ cutting of P. miscanthi teliospores by FESEM. However, no information is available on the ultrastructure of P. miscanthi in the teliospore stage during interaction with M. sinensis as observed by transmission electron microscopy.…”

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“…Approximately 2-m-tall eulalia grasses were grown in the mountainous region of Muju, Korea ( Kim, 2015 ). Mature leaves with typical leaf rust symptoms were collected from the middle part of the grasses in fall.…”

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“…Fragments of both uninfected and naturally infected M. sinensis leaves were fixed and processed as previously reported ( Kim, 2015 ; Seo et al, 2014 ). Briefly, they were immersed in a modified Karnovsky’s fixative in 0.05 M sodium cacodylate buffer at 4°C overnight.…”

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Ultrastructure of the Rust Fungus Puccinia miscanthi in the Teliospore Stage Interacting with the Biofuel Plant Miscanthus sinensis

Kim

2015

The Plant Pathology Journal

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Interaction of the the rust fungus Puccinia miscanthi with the biofuel plant Miscanthus sinensis during the teliospore phase was investigated by light and electron microscopy. P. miscanthi telia were oval-shaped and present on both the adaxial and abaxial leaf surfaces. Teliospores were brown, one-septate (two-celled), and had pedicels attached to one end. Transmission electron microscopy revealed numerous electron-translucent lipid globules in the cytoplasm of teliospores. Extensive cell wall dissolution around hyphae was not observed in the host tissues beneath the telia. Hyphae were found between mesophyll cells in the leaf tissues as well as in host cells. Intracellular hyphae, possibly haustoria, possessed electron-dense fungal cell walls encased by an electron-transparent fibrillar extrahaustorial sheath that had an electron-dense extrahaustorial membrane. The infected host cells appeared to maintain their membrane-bound structures such as nuclei and chloroplasts. These results suggest that the rust fungus maintains its biotrophic phase with most mesophyll cells of M. sinensis. Such a nutritional mode would permit the rust fungus to obtain food reserves for transient growth in the course of host alteration.

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Ultrastructure of the epiphytic sooty mold Capnodium and surface-colonized walnut leaves

Kim

2016

Eur J Plant Pathol

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Three-dimensional surface reconstruction and in situ site-specific cutting of the teliospores of Puccinia miscanthi causing leaf rust of the biomass plant Miscanthus sinensis

Cited by 6 publications

References 17 publications

Biomedical Applications of Stereoscopy for Three-Dimensional Surface Reconstruction in Scanning Electron Microscopes

Biomedical Applications of Stereoscopy for Three-Dimensional Surface Reconstruction in Scanning Electron Microscopes

Ultrastructure of the Rust Fungus Puccinia miscanthi in the Teliospore Stage Interacting with the Biofuel Plant Miscanthus sinensis

Ultrastructure of the epiphytic sooty mold Capnodium and surface-colonized walnut leaves

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