Three-dimensional wide-field pump-probe structured illumination microscopy

Abstract

We propose a new structured illumination scheme for achieving depth resolved wide-field pump-probe microscopy with sub-diffraction limit resolution. By acquiring coherent pump-probe images using a set of 3D structured light illumination patterns, a 3D super-resolution pump-probe image can be reconstructed. We derive the theoretical framework to describe the coherent image formation and reconstruction scheme for this structured illumination pump-probe imaging system and carry out numerical simulations to investigate its imaging performance. The results demonstrate a lateral resolution improvement by a factor of three and providing 0.5 µm level axial optical sectioning.

Three-dimensional wide-field pump-probe structured illumination microscopy: erratum

“…3 , e y z  of Eq. (5) in [1] has an error for the positive/negative signs in front of the unit vectors in y and z directions. The revised equation is as the following.…”

“…( ) The above-mentioned revision affects the plus/minus signs in the following equations and the revised equations for Eq. (6), (7), and (8) in [1] are shown below. The structured pump beam intensity for S-pol interference pattern (Eq.…”

“…, , e x y z  , we obtain the pump beam spectrum in the spatial-frequency domain (Eq. 7) in [1]) as , , ,…”

“…Substituting the previous result into Eq. (4) in [1], and mathematically expanding, we get the corresponding image field in the Fourier space (Eq. 8) in [1] This 3D CTF is an axially shifted cap of a paraboloid of revolution about the s axis in Fig.…”

“…1(a) (Fig. 10) in [1]). However, this axial shift of the 3D CTF comes from the wave vector of the incident light and the 3D CTF in Fig.…”