Three Draft Genome Sequences of
            <i>Staphylococcus</i>
            Species Isolated from the Urine of Healthy Bovine Heifers (Gyr Breed)

Giannattasio-Ferraz, Silvia; Maskeri, Laura; Oliveira, André Alves de; Barbosa-Stancioli, Edel Figueiredo; Putonti, Catherine

doi:10.1128/mra.00389-20

Cited by 5 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The human UT microbiota is the best studied UT microbiota amongst mammals. Isolation of strains from the bovine UT, including our prior work investigating other bacterial taxa ( Giannattasio-Ferraz et al, 2020a , b , c , d , e , 2021a ) and our study presented here, is a further step toward understanding the healthy bovine UT microbiota. Furthermore, here we have compared these bovine isolates to human UT isolates, producing the first examination of urinary bacteria and urinary phages from two different mammals.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, microbiome studies have found bacteria within the UT of other healthy mammals, including canines ( Burton et al, 2017 ) and swine ( Torres Luque et al, 2020 ). Furthermore, through our prior efforts, we have isolated several different bacterial taxa from the UT of healthy cattle ( Giannattasio-Ferraz et al, 2020a , b , c , d , e , 2021a ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

et al. 2022

Self Cite

View full text Add to dashboard Cite

The study of livestock microbiota has immediate benefits for animal health as well as mitigating food contamination and emerging pathogens. While prior research has indicated the gastrointestinal tract of cattle as the source for many zoonoses, including Shiga-toxin producing Escherichia coli and antibiotic resistant bacteria, the bovine urinary tract microbiota has yet to be thoroughly investigated. Here, we describe 5 E. coli and 4 Pseudomonas aeruginosa strains isolated from urine of dairy Gyr cattle. While both species are typically associated with urinary tract infections and mastitis, all of the animals sampled were healthy. The bovine urinary strains were compared to E. coli and P. aeruginosa isolates from other bovine samples as well as human urinary samples. While the bovine urinary E. coli isolates had genomic similarity to isolates from the gastrointestinal tract of cattle and other agricultural animals, the bovine urinary P. aeruginosa strains were most similar to human isolates suggesting niche adaptation rather than host adaptation. Examination of prophages harbored by these bovine isolates revealed similarity with prophages within distantly related E. coli and P. aeruginosa isolates from the human urinary tract. This suggests that related urinary phages may persist and/or be shared between mammals. Future studies of the bovine urinary microbiota are needed to ascertain if E. coli and P. aeruginosa are resident members of this niche and/or possible sources for emerging pathogens in humans.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…So many reports are supporting the results of our study. Giannattasio-Ferraz et al [25] they also demonstrated that most superabundant phyla are rmicutes that contain 40-50% of the diversity followed by Proteobacteria, Bacteroides, and Actinobacteria in vaginal micro ora. Differences in bacterial diversity were observed between these studies because of Breed, Feed, environmental condition, geological location, age, etc.…”

Section: Discussionmentioning

confidence: 94%

Dynamics of Vaginal Microbiota During Estrous Cycle in Cows through Metagenomic Approach

Purohit¹,

Patel²,

Gohil³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Using 16s rRNA sequencing of the V3-V4 hypervariable region, the present study is aimed to check vaginal microbiota diversity throughout different stages of the estrous cycle, with attention to hormonal changes and microorganism diversity. Metagenomic research was conducted on vaginal swab samples obtained from healthy cows' at different stages of the estrous cycle. Results: Total sixteen cows were synchronized with dobble PG regime. Nine cows demonstrated estrus within 96 hours and were eligible for the experiment. Vaginal samples for metagenomics and blood samples for hormonal analysis were collected during estrus, metestrus, diestrus and proestrus of synchronized Estrous cycle. The study's findings revealed that the diestrus phase has a different diversity than the other three estrous cycle phases, implying that hormones affect bacterial diversity. Proteobacteria and Firmicutes are the most abundant phyla at the phylum level, accounting for 94 % of bacterial diversity. Actinobacteriota, Patescibacteria, Cyanobacteria, Bacteroidota, and others are fewer common phyla. Proteobacteria are most common throughout the estrus, metestrus, and proestrus stages of the estrous cycle at the phylum level, there was no discernible distinction between the follicular and luteal phases. After statistical correction, Bacillaceae, Alcaligenes, and Enterobacteriaceae & Morganellacea families are more significant. At the diestrus stage, the Family Enterobacteriaceae is lower than at other stages; otherwise, all statistically significant genera are high at diestrus stages. The luteal phase had higher levels of Micrococcus, Stenotrophomonas, UGC-010, Massilia, and Methylobacillus than the follicular phase, however, statistical analysis revealed no substantial difference between the two phases. Lactobacillus genus is present on two samples including the estrus stage and diestrus stages. Conclusions: This study represents an important step towards the understanding of microbial diversity within different stages of the estrous cycle of the dairy cow. The study results revealed dynamics of metabiota during estrous cycle.

show abstract

“…The fact that C. phoceense has been primarily detected within the urinary tract of humans and now cattle suggests that this species may be specific to this environment. Recent studies have found that some of the same bacterial species inhabit both the bovine and human urinary microbiota [48,49]. Previous research found that the proteoglycan surface within the human and bovine bladder are similar [50]; in humans, this layer is a source for adhesion and nutrients by urinary bacteria [51].…”

Section: Discussionmentioning

confidence: 99%

Corynebacterium phoceense, resident member of the urogenital microbiota?

et al. 2021

Self Cite

View full text Add to dashboard Cite

Corynebacterium phoceense is a Gram-positive species previously isolated from human urine. Although other species from the same genus have been associated with urinary tract infections, C. phoceense is currently believed to be a non-pathogenic member of the urogenital microbiota. Prior to our study, only two isolates were described in the literature, and very little is known about the species. Here, we describe C. phoceense UFMG-H7, the first strain of this species isolated from the urine of healthy cattle. The genome for this isolate was produced and compared to the two other publicly available C. phoceense as well as other Corynebacterium genome assemblies. Our in-depth genomic analysis identified four additional publicly available genome assemblies that are representatives of the species, also isolated from the human urogenital tract. Although none of the strains have been associated with symptoms or disease, numerous genes associated with virulence factors are encoded. In contrast to related Corynebacterium species and Corynebacterium species from the bovine vaginal tract, all C. phoceense strains examined code for the SpaD-type pili suggesting adherence is essential for its persistence within the urinary tract. As the other C. phoceense strains analysed were isolated from the human urogenital tract, our results suggest that this species may be specific to this niche.

show abstract

Three Draft Genome Sequences of Staphylococcus Species Isolated from the Urine of Healthy Bovine Heifers (Gyr Breed)

Cited by 5 publications

References 7 publications

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens

Dynamics of Vaginal Microbiota During Estrous Cycle in Cows through Metagenomic Approach

Corynebacterium phoceense, resident member of the urogenital microbiota?

Contact Info

Product

Resources

About