2019
DOI: 10.1101/594697
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Three new integration vectors and fluorescent proteins for use in the opportunistic human pathogenStreptococcus pneumoniae

Abstract: Here we describe the creation of three integration vectors, pPEPX, pPEPY and pPEPZ, for use with the opportunistic human pathogen Streptococcus pneumoniae. The constructed vectors, named PEP for Pneumococcal Engineering Platform, employ an IPTG-inducible promoter and BglBrick and BglFusion compatible multiple cloning sites allowing for fast and interchangeable cloning. PEP plasmids replicate in Escherichia coli and harbor integration sites that have homology in a large set of pneumococcal strains, including re… Show more

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Cited by 6 publications
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