Three New Species, Two New Records and Four New Collections of Tubeufiaceae from Thailand and China

Abstract

Tubeufiaceae, a cosmopolitan family with a worldwide distribution, is mostly reported as saprobic on decaying woody materials from both aquatic and terrestrial habitats. The family is commonly found as helicosporous hyphomycetes, while some are chlamydosporous and phragmosporous. In this study, thirteen helicosporous hyphomycetes were collected from Thailand and China. The phylogenetic analyses of combined ITS, LSU, TEF1-α, and RPB2 sequence data placed them in Dematiohelicomyces, Helicoma, Helicotruncatum, Neohelicosporium, Parahelicomyces, and Tubeufia within Tubeufiaceae. Three new species, Tubeufia cocois, Parahelicomyces chiangmaiensis, and Neohelicosporium bambusicola, one new host record, Tubeufia laxispora, and one new geographic record, T. longihelicospora, are introduced based on both morphological characteristics and phylogenetic analyses. In addition, Dematiohelicomyces helicosporus, Helicoma guttulatum, Helicotruncatum palmigenum, and Tubeufia cylindrothecia are described with detailed descriptions and color photo plates.

Four new species and three new records of helicosporous hyphomycetes from China and their multi-gene phylogenies

“…The maximum likelihood and Bayesian analysis of the combined dataset resulted in phylogenetic reconstructions with largely similar topologies, and the IQ-Tree is shown in Figure 2. Representatives of the sequenced genera (with molecular data) of helicosporous hyphomycetes (Boonmee et al, 2011(Boonmee et al, , 2014Rajeshkumar and Sharma, 2013;Brahamanage et al, 2017;Doilom et al, 2017;Lu et al, 2017aLu et al, , 2018aLuo et al, 2017;Phookamsak et al, 2017;Liu et al, 2019;Tian et al, 2022) are included in our phylogenetic analysis (Figure 2). Thirty-six genera are represented by at least one species in Tubeufiaceae.…”

“…Notes: Helicoma guttulatum was introduced by Hyde et al ( 2016) with morphological and phylogenetic evidence. Tian et al (2022) reported a new collection from Thailand. In this study, three newly obtained isolates clustered with two known strains of H. guttulatum (MFLUCC 16-0022 and MFLUCC 21-0152) with high statistical support (100% ML/1.00 PP, Figure 2).…”

“…The difficulty in the taxonomic study of helicosporous hyphomycete species is that their morphological characteristics are very similar; it is difficult to distinguish them only by morphological comparison (Linder, 1929;Pirozynski, 1972;Goos, 1985Goos, , 1986Goos, , 1989Zhao et al, 2007;Kuo and Goh, 2018;Lu et al, 2018a,b;Hsieh et al, 2021;Tian et al, 2022). Therefore, polygenic phylogenetic analysis is required to accurately identify them.…”

“…The most remarkable feature that distinguishes helicosporous hyphomycetes from other fungal groups is that its conidia curve through at least 180 • in one plane as they extend in length (Goos, 1986;Zhao et al, 2007;Luo et al, 2017;Lu et al, 2018a,b;Tian et al, 2022). They are distributed in the Dothideomycetes (Capnodiales, Microthyriales, Pleosporales, Tubeufiales, and Venturiales), Leotiomycetes (Helotiales), Orbiliomycetes (Orbiliales), Sordariomycetes (Hypocreales, Lulworthiales, Microascales, Torpedosporales), Agaricomycetes (Agaricales), Atractiellomycetes (Atractiellales), Exobasidiomycetes (Exobasidiales), Tremellomycetes (Tremellales), and Zoopagomycetes (Zoopagales) (Lu and Kang, 2020).…”

“…Most species in this group, which were published more than 10 years ago, were saprophytic on terrestrial woody substrates, and most of them were lacking in DNA molecular data (Goos, 1986;Zhao et al, 2007;Boonmee et al, 2014;Lu et al, 2018b). However, the species of this group discovered in the last decade mainly come from aquatic habitats (Lu et al, 2018b;Boonmee et al, 2021;Tian et al, 2022), and almost all newly published helicosporous species have molecular data. The latest comprehensive revision on helicosporous hyphomycetes was carried out by Lu et al (2018b), who established nine new helicosporous genera based on morphology and phylogeny, viz.…”