Three Novel Antisense Overlapping Genes in E. coli O157:H7 EDL933

F, Graf; Zehentner, Barbara K.; Fellner, Lea; Scherer, Stephen W.; Neuhaus, Klaus

doi:10.1128/spectrum.02351-22

Cited by 1 publication

(1 citation statement)

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“…These results indicate that their regulatory network plasticity, potentially involving the altered expression of both canonical and noncanonical cellular components -from moonlighting proteins to transient translatomes ( 58 – 62 ), allows cells to adapt to various environments and even compensate for the loss of some gene functions ( 63 ). In turn, this adaptation allows nonlethal deleterious mutations to persist within the population for an extended time.…”

Section: Discussionmentioning

confidence: 99%

Nonlethal deleterious mutation–induced stress accelerates bacterial aging

Kohram,

Sanderson,

Loui

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Random mutagenesis, including when it leads to loss of gene function, is a key mechanism enabling microorganisms’ long-term adaptation to new environments. However, loss-of-function mutations are often deleterious, triggering, in turn, cellular stress and complex homeostatic stress responses, called “allostasis,” to promote cell survival. Here, we characterize the differential impacts of 65 nonlethal, deleterious single-gene deletions on Escherichia coli growth in three different growth environments. Further assessments of select mutants, namely, those bearing single adenosine triphosphate (ATP) synthase subunit deletions, reveal that mutants display reorganized transcriptome profiles that reflect both the environment and the specific gene deletion. We also find that ATP synthase α-subunit deleted ( ΔatpA ) cells exhibit elevated metabolic rates while having slower growth compared to wild-type (wt) E. coli cells. At the single-cell level, compared to wt cells, individual ΔatpA cells display near normal proliferation profiles but enter a postreplicative state earlier and exhibit a distinct senescence phenotype. These results highlight the complex interplay between genomic diversity, adaptation, and stress response and uncover an “aging cost” to individual bacterial cells for maintaining population-level resilience to environmental and genetic stress; they also suggest potential bacteriostatic antibiotic targets and -as select human genetic diseases display highly similar phenotypes, - a bacterial origin of some human diseases.

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