“…The methods used to purify different enzymes from other sources may not be suitable for a novel enzyme or source, because enzymes can be affected by various factors (ammonium sulfate concentration, ratio of tbutanol, pH and incubation time) in the method. Therefore, the method must be optimized, hence in the case of enzyme purification with TPP, the method has been optimized in the literature Chaiwut et al, 2010;Dhananjay and Mulimani, 2008;Duman and Kaya, 2013;Gagaoua et al, 2014;Hussain et al, 2009;Liu et al, 2006, Sagu et al, 2015.…”