Three-step Förster resonance energy transfer on an amyloid fibril scaffold

Gorbenko, Galyna; Zhytniakivska, Olga; Vus, Kateryna; Tarabara, Uliana; Trusova, Valeriya

doi:10.1039/d1cp01359a

Cited by 16 publications

(23 citation statements)

References 40 publications

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“…FRET systems involve the presence of a significant amount of chromophores able to mainly absorb light radiation, e.g., in the visible region of the spectrum (this is addressed in more detail below). Importantly, these artificial systems would be suitable for generating multi-step mechanism directional energy transfer of the absorbed radiation energy [88]. These chemical environment conditions would allow for reaching photoactive centers separated by distances in the order of nanometers.…”

Section: Photo-induced Energy Transfer Processesmentioning

confidence: 99%

Chromophoric Dendrimer-Based Materials: An Overview of Holistic-Integrated Molecular Systems for Fluorescence Resonance Energy Transfer (FRET) Phenomenon

et al. 2021

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Dendrimers (from the Greek dendros → tree; meros → part) are macromolecules with well-defined three-dimensional and tree-like structures. Remarkably, this hyperbranched architecture is one of the most ubiquitous, prolific, and recognizable natural patterns observed in nature. The rational design and the synthesis of highly functionalized architectures have been motivated by the need to mimic synthetic and natural-light-induced energy processes. Dendrimers offer an attractive material scaffold to generate innovative, technological, and functional materials because they provide a high amount of peripherally functional groups and void nanoreservoirs. Therefore, dendrimers emerge as excellent candidates since they can play a highly relevant role as unimolecular reactors at the nanoscale, acting as versatile and sophisticated entities. In particular, they can play a key role in the properties of light-energy harvesting and non-radiative energy transfer, allowing them to function as a whole unit. Remarkably, it is possible to promote the occurrence of the FRET phenomenon to concentrate the absorbed energy in photoactive centers. Finally, we think an in-depth understanding of this mechanism allows for diverse and prolific technological applications, such as imaging, biomedical therapy, and the conversion and storage of light energy, among others.

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Section: Photo-induced Energy Transfer Processesmentioning

confidence: 99%

Chromophoric Dendrimer-Based Materials: An Overview of Holistic-Integrated Molecular Systems for Fluorescence Resonance Energy Transfer (FRET) Phenomenon

et al. 2021

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“…It is of interest to investigate if the prepared materials can be employed for applications involving donor–acceptor pairs of dyes. There has been much interest recently in mimicking natural light-harvesting processes by achieving efficient energy transfer from donors to acceptors through the Förster resonance energy transfer (FRET) process. − Such an approach can moreover be employed to achieve systems capable of emission of white light.…”

Section: Introductionmentioning

confidence: 99%

Mechanochemical Preparation and Self-Assembly of Protein:Dye Hybrids for White Luminescence

Yuan

Solin

2021

ACS Appl. Polym. Mater.

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Protein nanofibrils (PNFs) functionalized with multiple dyes are prepared by a combination of mechanochemistry and liquid-phase self-assembly. The three employed dyes are Fluorescent Brightener 378 (F378), 2-butyl-6-(butylamino)-1 H -benzo[ de ]isoquinoline-1,3(2 H )-dione (Fluorol 555), and Nile red (NR). F378 acts as the donor with Fluorol 555 as the acceptor. F555 in turn acts as the donor and NR as the acceptor. This enables a FRET cascade that enables conversion of UV light to white light. The efficiency of FRET can be influenced by the details of the self-assembly process. If proteins milled with different dyes are mixed prior to self-assembly, nanofibrils are formed containing all three dyes, thus favoring FRET processes. By tuning the ratio of the three luminescent dyes, PNF dispersions are obtained that display bright white light emission. Moreover, the PNF dispersions can be converted into white luminescent films and gels where the PNFs may help to organize dye molecules. Additionally, the PNF materials can be employed as coatings on commercial LEDs, enabling emission of white light.

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“…During the last decades the phosphonium-based optical probes attract ever growing interest due to their favorable characteristics, namely, the excellent chemical and photophysical stability, high aqueous solubility, long wavelength absorption and emission, large extinction coefficient, high fluorescence quantum yield, low cytotoxicity, etc [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. The above properties render phosphonium dyes highly suitable for the selective staining of mitochondria [3][4][5], antioxidants detection [6,7], probing the structural differences of DNA/RNA grooves [1,2], exploring the tumor multidrug resistance [8], to name only a few.…”

mentioning

confidence: 99%

“…Moreover, the phosphonium conjugates proved to be very effective in monitoring the oxygen variations within mitochondria [10] and measuring the hydrogen peroxide levels [11]. Furthermore, numerous studies have demonstrated that phosphonium fluorescent dyes can be effectively used for optical detection of disease-related protein aggregates, amyloid fibrils [12,13]. In particular, a phosphonium dye TDV was used as a mediator in the amyloid-scaffolded multichromophoric systems for monitoring the amyloid transformation of the N-terminal fragment of apolipoprotein A-I [12] and insulin [13].…”

mentioning

confidence: 99%

“…Furthermore, numerous studies have demonstrated that phosphonium fluorescent dyes can be effectively used for optical detection of disease-related protein aggregates, amyloid fibrils [12,13]. In particular, a phosphonium dye TDV was used as a mediator in the amyloid-scaffolded multichromophoric systems for monitoring the amyloid transformation of the N-terminal fragment of apolipoprotein A-I [12] and insulin [13]. Moreover, previous studies suggested that phosphonium dyes may prove of value in elucidating the mechanism of DNA interactions with pathogenic protein aggregates [14].…”

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confidence: 99%

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Interactions of Novel Phosphonium Dye with Lipid Bilayers: A Fluorescence Study

Zhytniakivska

2021

EEJP

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The phosphonium-based optical probes attract ever growing interest due to their excellent chemical and photophysical stability, high aqueous solubility, long wavelength absorption and emission, large extinction coefficient, high fluorescence quantum yield, low cytotoxicity, etc. The present study was focused on assessing the ability of the novel phosphonium dye TDV to monitor the changes in physicochemical properties of the model lipid membranes. To this end, the fluorescence spectral properties of TDV have been explored in lipid bilayers composed of zwitterionic lipid phosphatidylcholine (PC) and its mixtures with cholesterol (Chol) or/and anionic phospholipid cardiolipin (CL). It was observed that in the buffer solution TDV possesses one well-defined fluorescence peak with the emission maximum at 533 nm. The dye transfer from the aqueous to lipid phase was followed by the enhancement of the fluorescence intensity coupled with a red shift of the emission maximum up to 67 nm, depending on the liposome composition. The quantitative information about the dye partitioning into lipid phase of the model membranes was obtained through approximating the experimental dependencies of the fluorescence intensity increase vs lipid concentration by the partition model. Analysis of the partition coefficients showed that TDV has a rather high lipid-associating ability and displays sensitivity to the changes in physicochemical properties of the model lipid membranes. The addition of CL, Chol or both lipids to the PC bilayer gives rise to the increase of the TDV partition coefficients compared to the neat PC membranes. The enhancement of the phosphonium dye partitioning in the CL and Chol-containing lipid bilayers has been attributed to the cardiolopin- and cholesterol-induced changes in the structure and physicochemical characteristics of the polar membrane region.

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Three-step Förster resonance energy transfer on an amyloid fibril scaffold

Abstract: The present study provides evidence that the energy transfer chain consisting of the benzothiazole dye Thioflavin T as an input donor, a phosphonium dye TDV and a squaraine dye SQ4...

Cited by 16 publications

References 40 publications

Chromophoric Dendrimer-Based Materials: An Overview of Holistic-Integrated Molecular Systems for Fluorescence Resonance Energy Transfer (FRET) Phenomenon

Chromophoric Dendrimer-Based Materials: An Overview of Holistic-Integrated Molecular Systems for Fluorescence Resonance Energy Transfer (FRET) Phenomenon

Mechanochemical Preparation and Self-Assembly of Protein:Dye Hybrids for White Luminescence

Interactions of Novel Phosphonium Dye with Lipid Bilayers: A Fluorescence Study

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