Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17&amp;lt;i&amp;gt;β&amp;lt;/i&amp;gt; and progesterone concentrations in preovulatory follicular fluid

Matsuo, Minami; Sumitomo, Kazuma; Ogino, Chihiro; Gunji, Yosuke; Nishimura, Ryo; Hishinuma, Mitsugu

doi:10.5194/aab-60-385-2017

Cited by 6 publications

(3 citation statements)

References 32 publications

(52 reference statements)

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“…The authors concluded that supplementation with P 4 during culture does not improve cleavage of presumptive zygotes, embryo development, and blastocyst rate. Matsuo et al (78) used a 3-step oocyte culture system that mimicked temporal physiological changes in E 2 and P 4 , and reported that the treatment enhanced in vitro embryo development reflected as a greater blastocyst formation rate. Reis et al (79) cultured zygotes in the following groups (concentrations in μg/mL): control, 1 E 2 + 100 P 4 , 1 E 2 , and 100 P 4 .…”

Section: Evidence On the Effect Of Hormonesmentioning

confidence: 99%

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

Lizarraga,

Gaxiola,

Castro del Campo

et al. 2024

Vet Mex OA

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We conducted a scoping review to 1) map and categorize published studies reporting interventions during the in vitro production of Bovine embryos and 2) qualitatively summarize the effects of treatments based on hormones, growth factors, sera, and reproductive fluids during oocyte maturation and embryo development. We searched electronic databases using keywords ('Bovine', 'embryo', 'blastocyst', 'oocyte', 'in vitro', 'quality') derived from the PIOS approach of the PRISMA statement. We identified 231 studies published during 2000−2021, with 133 being published between 2012 and 2021. The 231 studies were classified into four treatment categories: culture conditions (28), medium composition (45), bioactive supplements (79), and other additives (79), with 19 subcategories within these categories. A total of 77 studies included hormones, growth factors, sera, and reproductive fluids, among which 53 studies reported a positive effect on embryo production. Hormone-based treatments using melatonin, gonadotropins, and steroids were the most effective, followed by interventions assessing growth factors such as EGF, FGF, IGF-1, and BMP. These treatments improved oocyte competence, cytoplasmic/nuclear maturation, oocyte quality, and blastocyst development. Although free-serum media and synthetic/alternative products can partially or totally replace serum, it is sometimes necessary even at low concentrations. At high concentrations, hormones or growth factors may have detrimental effects on oocyte nuclear maturation, impair embryo development, and decrease embryo survival. Further meta-analyses are needed to estimate the intervention-specific effects. Future research should focus on interventions that have a positive effect and can be used extensively for Bovine production.

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Section: Evidence On the Effect Of Hormonesmentioning

confidence: 99%

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

Lizarraga,

Gaxiola,

Castro del Campo

et al. 2024

Vet Mex OA

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“…FF is an exudate of the blood serum and additionally contains growth factors and metabolic intermediates produced by the oocyte and somatic cells composing the follicle (Jiang et al, 2010;Sugiura, Pendola, & Eppig, 2005). Follicular somatic cells, more specifically the theca interna and mural granulosa cells, produce steroids, which are essential in sustaining oocyte and follicle development and survival and prepare the endometrium (Grupen & Armstrong, 2010;Matsuo et al, 2017;Okutsu, Itoh, Takahashi, & Ishizuka, 2010;Ting, Xu, & Stouffer, 2015). According to the two-cell type theory of ovarian steroidogenesis, theca cells convert cholesterol into progestins and androgens, while granulosa cells express FSHinduced aromatase (CYP19A1) to convert the androgens into 17β-estradiol (Liu & Hsueh, 1986).…”

Section: Introductionmentioning

confidence: 99%

Steroid profile of porcine follicular fluid and blood serum: Relation with follicular development

et al. 2019

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The aim of this study was to identify follicular fluid (FF) steroids which reflect follicular development in the early stages of the follicular phase and to establish whether the levels of these FF steroids correspond to their levels in serum. If these relations are established, serum steroid profiles may be used to monitor follicular development already in this early stage of the follicular phase. We used samples of two experiments, one with multiparous sows at the onset of the follicular phase (weaning) and one with primiparous sows at the midfollicular phase (48 hr after weaning). Complete steroid profiles were measured in pooled FF of the 15 largest follicles and serum using high‐performance liquid chromatography‐tandem mass spectrometry. In experiment 1, pooled FF volume, as a measure for average follicle size, tended to be positively related to higher FF 17β‐estradiol levels (β = 0.56, p = .08). In experiment 2, a larger FF volume was related not only to FF higher 17β‐estradiol levels (β = 2.11, p < .001) but also to higher levels of β‐nortestosterone (β = 1.15, p < .0001) and its metabolite 19‐norandrostenedione (β = 1.27, p < .01). In addition, FF volume was related to higher FF 17α‐OH‐pregnenolone (β = 1.63, p = .03) and 17α‐OH‐progesterone (β = 1.83, p < .001), which could indicate that CYP17,20‐lyase activity is limiting for 17β‐estradiol production in larger follicles at the beginning of the follicular phase. In serum, most of the steroids were present at lower levels compared to FF, except for the corticosteroids. Serum progestins and androgens were never related to follicle pool volume and steroid levels did not differ in the midfollicular phase compared to the onset of the follicular phase in the second experiment. Serum steroid levels therefore poorly reflect the developmental stage of the follicle pool in the first half of the follicular phase of the estrous cycle in sows.

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“…These effects suggest steroid concentrations during oocyte maturation in vitro are also important for oocyte developmental competency. Indeed, the addition of steroids in vitro has been shown to increase oocyte developmental competency and therefore represent another factor influencing the attainment of oocyte developmental competency in vitro (Ali & Sirard, 2002b;Aparicio et al, 2011;Matsuo et al, 2017;Soares et al, 2017;Younis et al, 1989). 1.3.14.4 Cholesterol In the follicle, mural granulosa cells synthesise steroids required for fertility (Hillier & Tetsuka, 1997;Hu et al, 2010;Li et al, 2000;Vanderhyden & Tonary, 1995).…”

Section: 3143mentioning

confidence: 99%

Refinement of a physiologically relevant IVM/IVF system on oocyte developmental competency in New Zealand dairy cows

Murray¹

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Fertility of high-producing dairy cows world-wide has declined over the last 50 years, with a decline observed in New Zealand (NZ) dairy cows during the 1990s and early 2000s. This decline is thought to be largely due to metabolic strain experienced during lactation, when peak milk production and mating coincide. It has been demonstrated that the major loss of pregnancy in NZ dairy cows across the 282 days of gestation occurs within the first 7 days following conception. This evidence suggests that the conditions associated with early lactation result in compromised oocyte quality and low conception rate. Experiments in which oocytes are obtained from dairy cows for research purposes are very expensive limiting the amount of research in this area. Thus, traditional bovine in vitro embryo production is utilised which produces embryos from oocytes obtained from abattoir ovaries. This process involves extracting immature oocytes from follicles and maturing them in vitro prior to fertilisation and embryo culture. One major limitation to this system is that it uses an in vitro maturation (IVM) media that is unreflective of the in vivo microenvironment in which the oocyte matures. Therefore, in order to better understand the factors affecting oocyte quality in the NZ dairy cow, a physiologically relevant IVM system needs to be developed. The overall objective of my PhD study was to develop a refined IVM culture system that better resembles the follicular microenvironment; and in comparison, to the gold-standard IVM system, test its impact on oocyte developmental competency by measuring known biomarkers of oocyte quality, blastocyst rate and indicators of embryo quality. New cumulus cell, oocyte and embryo-derived gene candidates were also associated with embryonic outcome in order to identify novel molecular biomarkers that could be used to predict oocyte quality. A bi-phasic physiologically-relevant (PR) IVM media system was developed to mimic the changing concentrations of specific constituents that had been measured within subordinate antral (‘Early’ media) and pre-ovulatory (‘Late’ media) follicles of NZ dairy cows. Additionally, each media were further divided into two media that consisted of either median (‘Good-PR’ media) or extreme-percentile (‘Bad-PR’ media; either 10th or 90th -percentil dependent on constituent) concentration values of each constituent, that was anticipated to represent good or poor quality follicles, respectively. Optimization experiments were conducted to test additional supplement requirements, as well as optimal timing of media changes and addition of sperm (fertilization) within the IVP protocol. All results were generated from two large IVP experiments. In brief, for all experiments, COCs were matured in individual culture drops containing either the Good-PR, Bad-PR or traditional (gold standard) IVM media to avoid cross-sample contamination and enable sample tracking. All COCs underwent media changes at 6 and 7.5 hours and after 22 hours of culture in conditions of 38°C and 5% CO2, were graded by morphology and biopsies of cumulus cells were collected for gene expression analyses. A group culture control was included in the first IVP experiment for a more accurate comparison to the traditional bovine IVP system. Following 22 hours in culture, remaining cumulus cells were dislodged, and all oocytes then followed traditional in vitro embryo production procedures. Resultant embryos were graded at Day 7-8 and any blastocysts were harvested and collected for either gene expression analyses or counting of total, healthy or pyknotic inner cell mass (ICM) and trophectoderm (TE) cell numbers. Overall, my results identified genes related to oocyte quality including four cumulus cell-derived (BMPR2, PDE8A, GJA1 and GATM) and four blastocyst-derived (ASNS, CDX2, PTGS2 and MSO1) genes. In addition, four genes related to cumulus cell expansion were also identified in cumulus cells (VCAN, PDE8A, BMPR2, GATM) and in the oocyte (HSP90B1). Interestingly, despite only minor differences in media constituent concentrations between the two PR IVM media, maturation of oocytes in the Bad-PR media consistently resulted in lower blastocyst rates. Intriguingly, there were no significant differences in blastocyst rate between the nutrient-rich Commercial and Good-PR IVM media. The Bad-PR IVM media also resulted in blastocysts with lower cell numbers than either the Good-PR or Commercial IVM media. Additionally, there was no differences in the number of cells of embryos created from oocytes matured in the Good-PR or Commercial IVM media. Critically however, there were fewer pyknotic cells present in embryos produced from oocytes matured in the Good-PR media compared to both the Bad-PR and Commercial media. Overall, the percentage of pyknotic ICM cells of embryos created from oocytes matured in the Good-PR treatment was lower than those created in the Commercial and Bad-PR IVM media. In summary, my results suggest that maturation of oocytes in concentrations of amino acids and metabolites at values within the outer extremes (i.e. 10th and 90th percentiles) of which are present in subordinate and pre-ovulatory follicles result in the production of low-quality oocytes associated with increased incidence of poor embryological development and reduced embryo quality. Importantly, this study also suggests that maturation of oocytes in concentrations of amino acids and metabolites at median values present in subordinate and pre-ovulatory follicles results in the production of high-quality oocytes associated with high quality embryos with fewer pyknotic ICM cells. Overall, this suggests that the small variations in constituent concentrations observed in follicular fluid of NZ dairy cows affects the quality of the oocytes developed. Further work should be undertaken in order to identify the specific constituent(s) (e.g. amino acids, glucose, cholesterol, fatty acids), and their threshold concentrations, which result in the alterations in oocyte quality. This information will allow a better understanding of the deficiencies within ovarian follicles that compromise oocyte quality which may lead to an intervention such as targeted feed management.

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Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17<i>β</i> and progesterone concentrations in preovulatory follicular fluid

Cited by 6 publications

References 32 publications

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

Steroid profile of porcine follicular fluid and blood serum: Relation with follicular development

Refinement of a physiologically relevant IVM/IVF system on oocyte developmental competency in New Zealand dairy cows

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