Threonine 308 within a Putative Casein Kinase 2 Site of the Cytoplasmic Tail of Leukotriene B4 Receptor (BLT1) Is Crucial for Ligand-induced, G-protein-coupled Receptor-specific Kinase 6-mediated Desensitization

Gaudreau, Rémi; Gouill, Christian Le; Venne, Marie-Hélène; Staňková, Jana; Rola‐Pleszczynski, Marek

doi:10.1074/jbc.m202723200

Cited by 54 publications

(66 citation statements)

References 66 publications

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“…On the other hand, coupling selectivity of BLT1 mutant receptors did not change (Ref. 33 and data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Structural Determinants of BLT1 C-tail Are Essential for Regulation of BLT1 Expression-We previously reported that a complete C-tail-truncated BLT1 (G291stop) showed increased numbers of [ 3 H]LTB 4 binding sites and a greater IP production in response to LTB 4 stimulation than WT BLT1 (33). In order to define whether selective BLT1 determinants could regulate its cell surface trafficking, we created two chimeric receptors composed of the main core of BLT1: residues 1-291 at the end of TM7 of BLT1 and the C-tail of either the PAFR or the LTD 4 receptor CysLT1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…2 (33) In addition, preliminary data revealed that a BLT1 deletion mutant (G291stop) lacking the C-tail, transiently expressed in COS-7 cells, showed higher numbers of LTB 4 binding sites and increased signal transduction when compared with the WT receptor (33). These data suggest that specific structural determinants of BLT1 C-tail regulate cell surface expression, yet the motifs involved are still unknown.…”

mentioning

confidence: 87%

“…In order to allow rapid assessment of cell surface expression of BLT1, the Nterminal initiator methionine was replaced by the Myc sequence MEQKLISEEDLSRGSPG resulting in Myc epitope-tagged WT and mutant BLT1. The G291stop mutant generation was also described previously (33). The chimeric mutant receptors were generated by PCR.…”

Section: Methodsmentioning

confidence: 99%

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Structural Determinants Regulating Expression of the High Affinity Leukotriene B4 Receptor

Gaudreau¹,

Beaulieu

Chen³

et al. 2004

Journal of Biological Chemistry

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Mutational analysis of determinants located in the C-terminal (C) tail of the high affinity leukotriene (LT)BA variety of stimuli, including light, neurotransmitters, hormones, and inflammatory lipid mediators produce their effects via activation of G-protein-coupled receptors (GPCRs). 1 Taking into account the heterogeneity of ligands, it is interesting that all members of this superfamily of receptors share the same topology characterized by the presence of seven transmembrane ␣-helices. Conserved residues within subfamily A (related to rhodopsin) are localized throughout these helices, serving as reference points for sequence alignments. Biophysical and biochemical evidence indicate that upon photoactivation, relative movements of transmembrane (TM) 3 and TM6 occur in rhodopsin; similarly, rearrangement of the cytoplasmic proximal portion of TM7 relative to TM1 and of TM2 relative to the intracellular helix VIII were also observed (reviewed in Ref.

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“…On the other hand, coupling selectivity of BLT1 mutant receptors did not change (Ref. 33 and data not shown).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

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Structural Determinants Regulating Expression of the High Affinity Leukotriene B4 Receptor

Gaudreau¹,

Beaulieu

Chen³

et al. 2004

Journal of Biological Chemistry

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“…PLB-985 cells were stably transfected with pcDNA3 vector containing a construct encoding for a myc-tagged BLT1 sequence (30). In short, 30 ϫ 10 6 cells were electroporated at 320 V using 30 g of pcDNA3-BLT.…”

Section: Cell Culture and Transfectionmentioning

confidence: 99%

Involvement of BLT1 Endocytosis and Yes Kinase Activation in Leukotriene B4-Induced Neutrophil Degranulation

Gaudreault

Thompson

Staňková

et al. 2005

The Journal of Immunology

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One of the important biological activities of human neutrophils is degranulation, which can be induced by leukotriene B4 (LTB4). Here we investigated the intracellular signaling events involved in neutrophil degranulation mediated by the high affinity LTB4 receptor, BLT1. Peripheral blood neutrophils as well as the promyeloid PLB-985 cell line, stably transfected with BLT1 cDNA and differentiated into a neutrophil-like cell phenotype, were used throughout this study. LTB4-induced enzyme release was inhibited by 50–80% when cells were pretreated with the pharmacological inhibitors of endocytosis sucrose, Con A and NH4Cl. In addition, transient transfection with a dominant negative form of dynamin (K44A) resulted in ∼70% inhibition of ligand-induced degranulation. Pretreating neutrophils or BLT1-expressing PLB-985 cells with the Src family kinase inhibitor PP1 resulted in a 30–60% inhibition in BLT1-mediated degranulation. Yes kinase, but not c-Src, Fgr, Hck, or Lyn, was found to exhibit up-regulated kinase activity after LTB4 stimulation. Moreover, BLT1 endocytosis was found to be necessary for Yes kinase activation in neutrophils. LTB4-induced degranulation was also sensitive to inhibition of PI3K. In contrast, it was not affected by inhibition of the mitogen-activated protein kinase MEK kinase, the Janus kinases, or the receptor tyrosine kinase epidermal growth factor receptor or platelet-derived growth factor receptor. Taken together, our results suggest an essential role for BLT1 endocytosis and Yes kinase activation in LTB4-mediated degranulation of human neutrophils.

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Biosynthesis, Biological Functions, and Receptors of Leukotriene B4 and 12(S)-Hydroxyheptadecatrienoic Acid

Okuno

Yokomizo

2019

Cutting-Edge Organic Synthesis and Chemical Biology of Bioactive Molecules

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Threonine 308 within a Putative Casein Kinase 2 Site of the Cytoplasmic Tail of Leukotriene B4 Receptor (BLT1) Is Crucial for Ligand-induced, G-protein-coupled Receptor-specific Kinase 6-mediated Desensitization

Cited by 54 publications

References 66 publications

Structural Determinants Regulating Expression of the High Affinity Leukotriene B4 Receptor

Structural Determinants Regulating Expression of the High Affinity Leukotriene B4 Receptor

Involvement of BLT1 Endocytosis and Yes Kinase Activation in Leukotriene B4-Induced Neutrophil Degranulation

Biosynthesis, Biological Functions, and Receptors of Leukotriene B4 and 12(S)-Hydroxyheptadecatrienoic Acid

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