Threshold Level and Traceability of Roundup Ready Soybean in Tofu Production

Nikolić, Zorica; Petrović, Gordana; Panković, Dejana; Ignjatov, Maja; Marinković, Dragana; Stojanović, Milan; Đorđević, Vuk

doi:10.17113/ftb.55.04.17.5192

Cited by 11 publications

(7 citation statements)

References 24 publications

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“…Among the five groups of samples, the RRS proportion in soya bean kernel prepared with 5% and 10% (w/w) RRS declined significantly ( p < 0.05) to 4.28% and 8.27%, respectively. This reduction of RRS content in the main product after mechanical manipulation was similar to those observed in previous studies, indicating that more damage was suffered by the exogenous target than by the lectin target [ 7 ]. Then, after defatting, the RRS proportions in soya bean oil and defatted soya bean kernel were higher than those in raw soya bean and soya bean kernel.…”

Section: Resultssupporting

confidence: 89%

“…To regulate the circulation of GMO products in the international market and help consumers make informed choices about foods that may contain GMO, many countries have established rules regarding the labelling of GMO products [ 5 , 6 ]. The rules in Japan, Thailand, Taiwan, South Africa and Indonesia state that any food product should be clearly labelled if it contains 5% or more GMO components [ 7 ]. The corresponding threshold values are 3% in South Korea [ 8 ] and 0.9% in the European Union and Russia [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Chen

et al. 2020

R. Soc. open sci.

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To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup Ready TM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Chen

et al. 2020

R. Soc. open sci.

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“…This reflects fragmentation of poppy DNA to very short fragments due to high degradation during baking. This is common for DNA extracted from a matrix that has undergone processing by high temperature [ 29 ] and a combination of grinding, mechanical manipulation, and thermal treatment [ 44 ]. However, the objective quality and usability of DNA extracted can only be revealed by its amplification.…”

Section: Resultsmentioning

confidence: 99%

Procedures for DNA Extraction from Opium Poppy (Papaver somniferum L.) and Poppy Seed-Containing Products

Kaňuková

Mrkvová

Mihálik

et al. 2020

Foods

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Several commonly used extraction procedures and commercial kits were compared for extraction of DNA from opium poppy (Papaver somniferum L.) seeds, ground seeds, pollen grains, poppy seed filling from a bakery product, and poppy oil. The newly developed extraction protocol was much simpler, reduced the cost and time required for DNA extraction from the native and ground seeds, and pollen grains. The quality of extracted DNA by newly developed protocol was better or comparable to the most efficient ones. After being extended by a simple purification step on a silica membrane column, the newly developed protocol was also very effective in extracting of poppy DNA from poppy seed filling. DNA extracted from this poppy matrix was amplifiable by PCR analysis. DNA extracted from cold-pressed poppy oil and suitable for amplifications was obtained only by methods developed previously for olive oil. Extracted poppy DNA from all tested matrices was analysed by PCR using primers flanking a microsatellite locus (156 bp) and two different fragments of the reference tubulin gene (553 bp and 96 bp). The long fragment of the reference gene was amplified in DNA extracted from native seeds, ground seeds, and pollen grains. Poppy DNA extracted from the filling of bakery product was confirmed only by amplification of short fragments (96 bp and 156 bp). DNA extracted from cold-pressed poppy oil was determined also only by amplification of these two short fragments.

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“…GMO quantitative analysis implies reliable detection of both endogenous and transgenic targets. Correspondingly, comparative analysis of their degradation during food processing is significant for the accurate Journal of Food Quality detection of GM foods [34][35][36]. Due to this evidence, we examined maize-specific and GMO-specific amplicons.…”

Section: Impact Of Heat On the Endogenous Ampliconsmentioning

confidence: 99%

Influence of Heat Processing on DNA Degradation and PCR-Based Detection of Wild-Type and Transgenic Maize

Bitskinashvili

Gabriadze²,

Kutateladze³

et al. 2019

Journal of Food Quality

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Reliable detection of genetically modi ed (GM) maize is signi cant for food authenticity, labelling, quality, and safety assessment.is study aims to evaluate the factors in uencing degradation and polymerase chain reaction (PCR) ampli cation of DNA from the wild type and transgenic maize (events Bt-176 and MON810) during thermal treatment at 100°C and 121°C. A new PCR method was developed targeting the Cry1Ab gene to detect insect-resistant GM plants. e yield of genomic DNAs extracted by the DNeasy plant mini kit dramatically decreased while DNAs obtained by cetyltrimethyl ammonium bromide-(CTAB-) based method did not show any visible changes in the yield by the time of processing. Treatment at 100°C did not signi cantly a ect either genomic DNAs or amplicons. Heating at 121°C induced time-dependent degradation of genomic DNAs and exogenous Cry1Ab gene; however, it did not have any considerable in uence on the exogenous 141 bp amplicons or endogenous amplicons in the range of 102 bp to 226 bp with the exception of the event MON810 extracted by the DNeasy plant mini kit. More yield was observed at 226 bp than 140 bp fragment of the invertase gene. e 141 bp fragment of the transgenic CaMV 35S promoter exhibited the highest thermal stability of all the examined amplicons. Analysis of foodstu s demonstrated 102 bp amplicons speci c for the zein gene as the e ective marker to detect maize in the processed foods. e obtained results demonstrate that PCR-based detection of the wild type and transgenic maize is dependent on the combination of di erent parameters of crucial factors such as temperature and duration of exposure, transgenic event, DNA extraction method, DNA marker, and size and location of amplicons.

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Threshold Level and Traceability of Roundup Ready Soybean in Tofu Production

Cited by 11 publications

References 24 publications

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Procedures for DNA Extraction from Opium Poppy (Papaver somniferum L.) and Poppy Seed-Containing Products

Influence of Heat Processing on DNA Degradation and PCR-Based Detection of Wild-Type and Transgenic Maize

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