Thrombin regulates monocyte chemoattractant protein-1 expression in human first trimester and term decidual cells

Matta, Paul; Lockwood, Charles J.; Schatz, Frederick; Krikun, Graciela; Rahman, Mizanur; Buchwalder, Lynn; Norwitz, Errol R.

doi:10.1016/j.ajog.2006.09.008

Cited by 20 publications

(13 citation statements)

References 27 publications

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“…This increase in proinflammatory chemokines are expected to result in endothelial dysfunction or inappropriate endothelial cell activation which are the most common clinical manifestations in PE, including enhanced endothelial-cell permeability and platelet aggregation [80]. Moreover, the overexpression of these chemokines is consistent with observations made in our laboratory [81, 82] as well as others [83, 84] indicating that the preeclamptic decidua contains an excess of macrophages [82]. …”

Section: Thrombin Activation Of Endometrial Endothelial Cellssupporting

confidence: 76%

Preeclampsia, Hypoxia, Thrombosis, and Inflammation

Shamshirsaz

Paidas

Krikun

2012

Journal of Pregnancy

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Section: Thrombin Activation Of Endometrial Endothelial Cellssupporting

confidence: 76%

Preeclampsia, Hypoxia, Thrombosis, and Inflammation

Shamshirsaz

Paidas

Krikun

2012

Journal of Pregnancy

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“…Western blotting for TLR-2 (diluted 1:1000) and β-actin (diluted 1:10,000) was conducted (n=1) using established methods (31). Antibodies for TLR-2 and β-actin were obtained from R&D Systems (Minneapolis, MN) and Abcam, Inc. (Cambridge, MA), respectively.…”

Section: Methodsmentioning

confidence: 99%

A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells

Paidas¹,

Krikun²,

Huang³

et al. 2010

American Journal of Obstetrics and Gynecology

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112

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OBJECTIVE-Preimplantation factor (PIF) is a novel, 15 amino acid peptide, secreted by viable embryos. This study aims to elucidate PIF's effects in human endometrial stromal cells (HESC) decidualized by estrogen and progestin, which mimics the pre-implantation milieu, and in first trimester decidua cultures (FTDC).STUDY DESIGN-HESC or FTDC were incubated with 100nM synthetic PIF or vehicle control. Global gene expression was analyzed using microarray and pathway-analysis. Proteins were analyzed using quantitative mass-spectrometry, and PIF binding by ProtoArray. RESULTS-Gene and proteomic analysis demonstrate that PIF affects immune, adhesion and apoptotic pathways. Significant upregulation in HESC (fold-change) include: NF-k-β activation via IRAKBP1 (53); TLR5 (9); FKBP15 protein (2.3); DSCAML1 (16). BCL-2 was downregulated in HESC (21.1) and FTDC (27.1). ProtoArray demonstrates PIF interaction with intracellular targets insulin degrading enzyme and beta-K+ channels.CONCLUSION-PIF displays essential multi-targeted effects, of regulating immunity, promoting embryo-decidual adhesion, and regulating adaptive apoptotic processes.

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“…CCL2 is expressed in human decidua throughout gestation [28,30]; however, approximately 50 chemokines exist and frequently act in conjunction, involved in sequential leucocyte intravasation, homing, and activation. An extensive study of chemokines is therefore required to identify key chemokines coordinating inflammatory events during TL and PTL.…”

Section: Decidual Leucocytes In Labor and Preterm Labormentioning

confidence: 99%

Macrophages Infiltrate the Human and Rat Decidua During Term and Preterm Labor: Evidence That Decidual Inflammation Precedes Labor1

Hamilton

Oomomian

Stephen

et al. 2012

Biology of Reproduction

245

214

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Preterm delivery is the leading cause of perinatal mortality and morbidity. Current tocolytics target myometrial contractions, a late step in the labor cascade. Identifying earlier events in parturition may lead to more effective therapeutic strategies. We hypothesized that inflammatory events in decidua (the maternal-fetal interface), characterized by leucocyte infiltration, are an early event during term and preterm labor (PTL). Leucocyte abundance in decidua of human pregnancies was quantified following term labor and PTL (idiopathic and infection associated), in conjunction with investigation of temporal inflammatory events in rat uterus during the perilabor period and in PTL induced by mifepristone. In human decidua, macrophage numbers were 4-fold higher in term labor (P < 0.01) and 2.5-fold higher in non-infection-associated PTL (P < 0.05) than in term nonlaboring samples. Neutrophil abundance was unchanged with labor but elevated in PTL with infection (5- to 53-fold increase; P < 0.01). T and NK cells were more abundant in idiopathic PTL than TL (P < 0.05). In rat, decidual macrophage infiltration increased 4.5-fold 12 h prior to labor and remained elevated during labor and early postpartum (P < 0.01). Decidual infiltration preceded that of the myometrium and was 4-fold higher (P < 0.01). In rat PTL, decidual macrophage numbers were also elevated (P < 0.01) and exceeded those of the myometrium (P < 0.05). These studies show for the first time that leucocytes infiltrate decidua during labor at term and preterm, supporting a role for leucocyte-derived inflammatory mediators in decidual activation. In the rat, this occurred prior to labor, suggesting it is an early event during parturition and thus a potential target for intervention.

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Thrombin regulates monocyte chemoattractant protein-1 expression in human first trimester and term decidual cells

Cited by 20 publications

References 27 publications

Preeclampsia, Hypoxia, Thrombosis, and Inflammation

Preeclampsia, Hypoxia, Thrombosis, and Inflammation

A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells

Macrophages Infiltrate the Human and Rat Decidua During Term and Preterm Labor: Evidence That Decidual Inflammation Precedes Labor1

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