Thurincin H Is a Nonhemolytic Bacteriocin of Bacillus thuringiensis with Potential for Applied Use

Ortiz-Rodríguez, Tomás; Mendoza-Acosta, Fernanda; Martínez-Zavala, Sheila A.; Salcedo‐Hernández, Rubén; Casados‐Vázquez, Luz E.; Bideshi, Dennis K.; Barboza‐Corona, José E.

doi:10.1007/s12602-022-09952-2

Cited by 6 publications

(5 citation statements)

References 56 publications

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“…Some SMs found in our MAGs such as Gramicidin, Pristinamycin, Rifamycin, Bacitracin, Thurincin H, and Bicereucin have been found to have antibacterial activity (Huo & Van Der Donk, 2016 ; Katz & Baltz, 2016 ; Ortiz‐Rodríguez et al, 2023 ; Singh et al, 2019 ; Sušnik & Koert, 2020 ). Other natural products BGCs found in our study such as Iturin, candicidin, Amphotericin, Nystatin, Pimaricin, thiocoraline, Syringomycin have been reported to have antifungal properties (Brautaset et al, 2008 ; Haeder et al, 2009 ; Katz & Baltz, 2016 ; Kawasaki et al, 2016 ; Qi et al, 2015 ; Son et al, 2016 ).…”

Section: Discussionmentioning

confidence: 82%

“…Exploration in silico of microbial communities of water and sediments of the karst ecosystem on the Yucatán peninsula revealed multidrug resistance genes associated with antibiotics such as erythromycin, fosfomycin, streptothricin, indicating that these microbial communities may be producing these antimicrobials or are exposed to them in this environment (Moore et al, 2020). Some SMs found in our MAGs such as Gramicidin, Pristinamycin, Rifamycin, Bacitracin, Thurincin H, and Bicereucin have been found to have antibacterial activity (Huo & Van Der Donk, 2016;Katz & Baltz, 2016;Ortiz-Rodríguez et al, 2023;Singh et al, 2019;Sušnik & Koert, 2020). Other natural products BGCs found in our study such as Iturin, candicidin, Amphotericin, Nystatin, Pimaricin, thiocoraline, Syringomycin have been reported to have antifungal properties (Brautaset et al, 2008;Haeder et al, 2009;Katz & Baltz, 2016;Kawasaki et al, 2016;Qi et al, 2015;Son et al, 2016).…”

Section: Biotechnological and Medical Importance Of The Bgcs And Sms ...mentioning

confidence: 85%

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Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Suárez‐Moo,

Prieto‐Davó

2024

MicrobiologyOpen

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Microbial communities from various environments have been studied in the quest for new natural products with a broad range of applications in medicine and biotechnology. We employed an enrichment method and genome mining tools to examine the biosynthetic potential of microbial communities in the sediments of a coastal sinkhole within the karst ecosystem of the Yucatán Peninsula, Mexico. Our investigation led to the detection of 203 biosynthetic gene clusters (BGCs) and 55 secondary metabolites (SMs) within 35 high‐quality metagenome‐assembled genomes (MAGs) derived from these subcommunities. The most abundant types of BGCs were Terpene, Nonribosomal peptide‐synthetase, and Type III polyketide synthase. Some of the in silico identified BGCs and SMs have been previously reported to exhibit biological activities against pathogenic bacteria and fungi. Others could play significant roles in the sinkhole ecosystem, such as iron solubilization and osmotic stress protection. Interestingly, 75% of the BGCs showed no sequence homology with bacterial BGCs previously reported in the MiBIG database. This suggests that the microbial communities in this environment could be an untapped source of genes encoding novel specialized compounds. The majority of the BGCs were identified in pathways found in the genus Virgibacillus, followed by Sporosarcina, Siminovitchia, Rhodococcus, and Halomonas. The latter, along with Paraclostridium and Lysinibacillus, had the highest number of identified BGC types. This study offers fresh insights into the potential ecological role of SMs from sediment microbial communities in an unexplored environment, underscoring their value as a source of novel natural products.

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Section: Discussionmentioning

confidence: 82%

Section: Biotechnological and Medical Importance Of The Bgcs And Sms ...mentioning

confidence: 85%

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Suárez‐Moo,

Prieto‐Davó

2024

MicrobiologyOpen

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“…Thurincin H was firstly produced and purified by culturing the Bacillota strain Bacillus thuringiensis SF361, leading to the identification of its GC composition [ 53 ]. By gene cloning the GC containing the structural genes and other genes involved in transport and immunity in Bacillus thuringiensis , thurincin H was successfully expressed and thereby purified in another study [ 54 ]. Additionally, using a plasmid-encoded precursor peptide, Wang et al [ 55 ] developed a homologous thurincin H expression system that effectively produces high levels of the bacteriocin from a single structural gene.…”

Section: Discussionmentioning

confidence: 99%

“…; Staphylococcus spp. ; and Listeria spp., such as the human pathogen and Listeria monocytogens , related to fish [ 18 , 54 , 59 ]. Lichenicidin VK21A2, a lanthipeptide identified in our study, was found previously to be effective against Listeria monocytogenes and two antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

In Silico Screening of Bacteriocin Gene Clusters within a Set of Marine Bacillota Genomes

Teber,

Asakawa

2024

IJMS

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Due to their potential application as an alternative to antibiotics, bacteriocins, which are ribosomally synthesized antimicrobial peptides produced by bacteria, have received much attention in recent years. To identify bacteriocins within marine bacteria, most of the studies employed a culture-based method, which is more time-consuming than the in silico approach. For that, the aim of this study was to identify potential bacteriocin gene clusters and their potential producers in 51 marine Bacillota (formerly Firmicutes) genomes, using BAGEL4, a bacteriocin genome mining tool. As a result, we found out that a majority of selected Bacillota (60.78%) are potential bacteriocin producers, and we identified 77 bacteriocin gene clusters, most of which belong to class I bacteriocins known as RiPPs (ribosomally synthesized and post-translationally modified peptides). The identified putative bacteriocin gene clusters are an attractive target for further in vitro research, such as the production of bacteriocins using a heterologous expression system.

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“…Turicin H, which has inhibitory activity against B. cereus, Bt Cry-B, wild-type E. faecium, and E. faecium ATCC, but not Bt Cry-B/pThurH and Bt Cry-B/pThurH∆ThnA, did not have hemolytic activity at a concentration of 20 µg/mL, which is four times higher than the minimum inhibitory concentration (MIC). This suggests the potentially safe use of turincin H as an antibacterial peptide for medical use [148]. While most bacteriocins are non-toxic to eukaryotic cells, enterococcal cytolysin has been found to be toxic [149].…”

Section: Bioavailability and Safety Of Bacteriocinsmentioning

confidence: 99%

Immunomodulation, Bioavailability and Safety of Bacteriocins

Guryanova

2023

Life

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The rise of antibiotic-resistant bacteria and the emergence of new pathogens have created a need for new strategies to fight against infectious diseases. One promising approach is the use of antimicrobial peptides produced by a certain species of bacteria, known as bacteriocins, which are active against other strains of the same or related species. Bacteriocins can help in the treatment and prevention of infectious diseases. Moreover, bacteriocins can be obtained in prokaryotic organisms, and contribute s to their widespread use. While the use of bacteriocins is currently limited to the food industry (for example, nisin is used as a preservative, E234), a large number of studies on their microbicidal properties suggest that their use in medicine may increase in the foreseeable future. However, for the successful use of bacteriocins in medicine, it is necessary to understand their effect on the immune system, especially in cases where immunity is weakened due to infectious processes, oncological, allergic, or autoimmune diseases. Studies on the immuno-modulatory activity of bacteriocins in animal models and human cells have revealed their ability to induce both pro-inflammatory and anti-inflammatory factors involved in the implementation of innate immunity. The influence of bacteriocins on acquired immunity is revealed by an increase in the number of T-lymphocytes with a simultaneous decrease in B-lymphocyte levels, which makes them attractive substances for reducing inflammation. The widespread use of bacteriocins in the food industry, their low toxicity, and their broad and narrow specificity are reasons for researchers to pay attention to their immunomodulatory properties and explore their medical applications. Inflammation regulation by bacteriocins can be used in the treatment of various pathologies. The aim of the review was to analyze scientific publications on the immunomodulatory activity, bioavailability, and safety of bacteriocins in order to use the data obtained to organize preclinical and clinical studies.

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Thurincin H Is a Nonhemolytic Bacteriocin of Bacillus thuringiensis with Potential for Applied Use

Cited by 6 publications

References 56 publications

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

In Silico Screening of Bacteriocin Gene Clusters within a Set of Marine Bacillota Genomes

Immunomodulation, Bioavailability and Safety of Bacteriocins

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