Thymic Hormones, Antioxidant Enzymes and Neurogenesis in Bulbus Olfactorius of Rats with Hemiparkinsonism: Effect of Melatonin

Labunets, I. F.; Talanov, Sergiy O.; Vasiliev, R. H.; Rodnichenko, A. E.; Utko, N.; Kuz'minov, I. A.; Kopyak, Bogdan S.; Pod'yachenko, O. V.; Сагач, В. Ф.; Butenko, H. M.

doi:10.1615/intjphyspathophys.v7.i4.10

Cited by 7 publications

(6 citation statements)

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“…In our study, the degree of immune disturbances in experimental parkinsonism may be connected with the pronouncement of the decrease of blood thymulin level [48]. The thymic endocrine function was restored after melatonin administration [48].…”

Section: Parkinson 'S Diseasementioning

confidence: 52%

Age-Related Changes in the Melatonin and Thymulin Biorhythms as Risk Factors for Human Neurodegenerative Diseases

Labunets¹

2017

GGS

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MelatoninNumerous studies suggest that melatonin is the main regulator of circadian and circannual rhythms both in mammals and humans CRIMSONpublishers http://www.crimsonpublishers.com Irina F Labunets* AbstractThe frequency of age-related neurodegenerative pathology, such as Parkinson's and Alzheimer's diseases, brain ischemia and multiple sclerosis increases worldwide and has great socio-economic implications. The use of approaches based on investigation the pathogenic factors of their development may be perspective in the prevention and treatment of such diseases.Circadian and circannual rhythms play a key role in the adaptative changes of human neuroendocrine and immune systems to the light and temperature. The rhythmicity of these systems is disturbed in human aging that may be connected with desynchronosis of pineal gland melatonin production. This process becomes more intensive during development of the neurodegenerative diseases.Thymic endocrine function (thymulin production) is the important part of chronobiological organization of immune system. The circadian and circannual rhythms of thymulin depend on the synchronizing influence of melatonin. Melatonin production disorders lead to the intra-immune desynchronosis via disturbances of the circadian and circannual rhythms of thymulin and its interactions with the endocrine glands (hypophysis, adrenal glands, gonads etc) both in aging and age pathology.Melatonin treatment improves sleep-wake rhythm and produces antiapoptotic, antioxidant and anti-inflammatory effects in the brain at neurodegenerative diseases and slows down development of this pathology. Moreover, melatonin treatment leads to the suppression of age-related changes in the rhythmicity of immune and neuroendocrine systems functions. Such melatonin effect is realized via synchronous influence on the circadian and circannual rhythms of thymulin production.Therefore melatonin seems to be perspective for restoring disturbed rhythmicity of immune and neuroendocrine systems in:A. accelerated human aging with pineal gland dysfunction and B. Patients of different age and sex suffering from neurodegenerative diseases making individual hormone dose correction.

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Section: Parkinson 'S Diseasementioning

confidence: 52%

Age-Related Changes in the Melatonin and Thymulin Biorhythms as Risk Factors for Human Neurodegenerative Diseases

Labunets¹

2017

GGS

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“…The level of malondialdehyde (MDA) in the mice brain was evaluated according to the intensity of the coloured trimethine complex formed between MDA and tiobarbituric acid (Sigma-Aldrich, Germany) and expressed in nM/mg [23]. The activity of superoxide dismutase (SOD) and catalase was determined in the supernatants of the homogenates of the murine brain [24]. To determine the activity of SOD, a method based on the ability of the enzyme to suppress the adrenaline auto-oxidation in adrenochrome (Sigma-Aldrich, Germany) at pH = 10.2 was used.…”

Section: Schemes Of the Use Of Rhil-10 And Rhfgf-2mentioning

confidence: 99%

The effects of interleukin-10 and fibroblasts growth factor-2 in mice with toxic cuprizone model of demyelination

Labunets¹,

Rodnichenko²,

Utko³

et al. 2019

JCOT

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Cytokines and growth factors exhibit neurotropic, anti-inflammatory and immunomodulatory properties, and therefore can affect the functioning of the nervous system at demyelinating disorders.Purpose. To identify changes in T-lymphocytes, macrophages, oxidative stress and antioxidant defence factors, endocrine thymus function in the brain and behaviour of mice receiving neurotoxin cuprizone and recombinant human proteins: interleukin-10 (rhIL-10) and fibroblast growth factor (rhFGF-2).Materials and methods. 4-6-month-old 129/Sv mice received cuprizone with food every day for 3 weeks. From the 7th day of cuprizone diet, they received different doses of rhIL-10 and rhFGF-2. The content of СD3+ Т-cells, macrophages, malondialdehyde, activity of antioxidant enzymes in the brain and the level of thymulin in the blood were determined. Behavioural reactions were assessed in the “open field” test.Results. In the brain of mice receiving cuprizone and rhIL-10, there was a decrease in the number of СD3+ Т-cells and the activity of macrophages, which significantly increased under the influence of the toxin. After cytokine injection, the activity of superoxide dismutase, catalase and glutathione peroxidase increased significantly in the brain, as well as the level of thymulin in the blood. Motor, emotional and exploratory activity of mice, significantly suppressed by the cuprizone, was increased after the introduction of rhIL-10. The effect of rhIL-10 on the test parameters is more pronounced at a dose of 5 μg/kg than 50 μg/kg. After injection of rhFGF-2 in the mice with cuprizone diet, there is a decrease in the activity of brain macrophages and an increase of the level of thymulin in the blood depending on the dose of this factor; the motor activity of these animals increased regardless of the rhFGF-2 dose.Conclusion. The injections of rhIL-10 and rhFGF-2 provide dose-dependent positive effects on the pathogenetic factors of experimental demyelinating pathology, as well as the functional state of the nervous system. Whereas, the injections of rhIL-10 have more pronounced effects.

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“…It is possible that such changes in the Nestin + cells can be explained by an increase in their differentiation in the neuronal direction, since the number of unchanged neurons simultaneously increase in such animals. A similar trend in the change in the number of such cells in the neurogenic area of the brain (olfactory bulb) with the simultaneous increase in the number of unchanged neurons in the middle brain was observed in animals with experimental hemiparkinsonism, which received melatonin [23,38]. In addition, exogenous melatonin increases the content of myelin-producing cells in the brain of the mice with a cuprizone demyelination model [8,14,32,33].…”

Section: The Content Of Nestin + Cells In the Brain And Morpho-functimentioning

confidence: 60%

Neuroprotective effect of melatonin in mice with toxic cuprizone model of demyelination and possible pathways of its realization

Labunets¹,

Rodnichenko²,

Melnyk³

et al. 2018

JCOT

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The search for tools that increase the effectiveness of cell therapy of demyelinating pathology is relevant. They may be preparations that affect the pathogenetic factors of this pathology, in particular, the pineal hormone melatonin.The purpose of the work is to evaluate the involvement of immune system and antioxidant defense in the implementation of the protective effects of melatonin on morpho-functional disorders in the central nervous system induced by neurotoxin cuprizone.Materials and methods. The toxic demyelination model was induced on 129/Sv mice at the age of 3-5 months by adding cuprizone to food for 3 weeks. Since the 7th day of cuprizone administration, melatonin was injected intraperitoneally at 18:00 daily, at a dose of 1 mg/kg. In the brain of mice, the proportion of CD3+, Nestin+ cells and phagocytic macrophages, the content of malondialdehyde and the activity of antioxidant enzymes was determined. Blood serum was tested for thymic hormone thymulin levels. In the animals, we evaluated the structure of the brain and spinal cord neurons by Nissl staining of histological sections as well as analyzed behavioural reactions in the "open field" test.Results. In the brain of the mice received cuprizone, the proportion of CD3+ and Nestin+ cells, active macrophages and malondialdehyde content increases, glutathione peroxidase and glutathione reductase levels decreases. In the brain and spinal cord of the mice with a cuprizone diet, the proportion of altered neurons increases, and motor and emotional activity decreases. The introduction of melatonin results in a decrease in the relative number of CD3+ cells, active macrophages and malondialdehyde content, increased activity of glutathione peroxidase, glutathione reductase and level of thymulin. In such mice, the proportion of unchanged neurons increases as the number of Nestin+ cells decreases and behavioural responses are also improved.Conclusions. The neuroprotective effect of melatonin in demyelinating pathology is realized through the factors of the immune system and oxidative stress. The results may be useful in the development of new biotechnological approaches to the treatment of demyelinating pathology, in particular, multiple sclerosis.

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Thymic Hormones, Antioxidant Enzymes and Neurogenesis in Bulbus Olfactorius of Rats with Hemiparkinsonism: Effect of Melatonin

Cited by 7 publications

References 0 publications

Age-Related Changes in the Melatonin and Thymulin Biorhythms as Risk Factors for Human Neurodegenerative Diseases

Age-Related Changes in the Melatonin and Thymulin Biorhythms as Risk Factors for Human Neurodegenerative Diseases

The effects of interleukin-10 and fibroblasts growth factor-2 in mice with toxic cuprizone model of demyelination

Neuroprotective effect of melatonin in mice with toxic cuprizone model of demyelination and possible pathways of its realization

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