Thymoquinone loading into hydroxyapatite/alginate scaffolds accelerated the osteogenic differentiation of the mesenchymal stem cells

Abstract

Background Phytochemical agents such as thymoquinone (TQ) have osteogenic property. This study aimed to investigate the synergic impact of TQ and hydroxyapatite on mesenchymal stem cell differentiation. Alginate was also used as drug vehicle. Methods HA scaffolds were fabricated by casting into polyurethane foam and sintering at 800 °C, and then, 1250 °C and impregnated by TQ containing alginate. The adipose-derived stem cells were aliquoted into 4 groups: control, osteogenic induced-, TQ and osteogenic induced- and TQ-treated cultures. Adipose derived-mesenchymal stem cells were mixed with alginate and loaded into the scaffolds Results The results showed that impregnation of HA scaffold with alginate decelerated the degradation rate and reinforced the mechanical strength. TQ loading in alginate/HA had no significant influence on physical and mechanical properties. Real-time RT-PCR showed significant elevation in collagen, osteopontin, and osteocalcin expression at early phase of differentiation. TQ also led to an increase in alkaline phosphatase activity. At long term, TQ administration had no impact on calcium deposition and proliferation rate as well as bone-marker expression. Conclusion TQ accelerates the differentiation of the stem cells into the osteoblasts, without changing the physical and mechanical properties of the scaffolds. TQ also showed a synergic influence on differentiation potential of mesenchymal stem cells.

Utilization of Nanotechnology to Improve Bone Health in Osteoporosis Exploiting Nigella sativa and Its Active Constituent Thymoquinone

“…The study showed the synergistic action of TQ and hydroxyapatite to accelerate and boost the osteogenic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs). The study also supports the positive role of TQ in bone metabolism [ 109 ]. Table 1 summarizes the pharmacological benefits of different components of NS (seed extract and seed oil) studied in various In vitro cell lines and preclinical investigations in the effective management of osteoporosis.…”

pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosusGG

“…The intact shape of Alg hydrogel in the stomach was possibly due to prolonged gelation time during a manufacturing process. Although the HAp/Alg composite hydrogel potentially has reinforced greater mechanical stability than the Alg hydrogel 37 owing to its denser structure and pH‐triggered Ca 2+ ion replenishment, there was no observable difference in bead shape in the stomach. Six hours after administration, the beads of both hydrogels had completely disintegrated in the intestines.…”

Expression and ratio of receptor activator of nuclear factor kappa-Β ligand and osteoprotegerin following application of Nigella sativa/bovine bone graft combination in post tooth extraction sockets

“…This suggests that the administration of N. sativa and bone graft can reduce the number of osteoclasts, increase the number of osteoblasts, and induce the process of bone homeostasis toward bone apposition in postextraction alveolar bone sockets. This finding is in line with those of studies conducted by Rahmani-Moghadam et al .,[ 26 ] who discovered that treating mesenchymal stem cells with a mixture of thymoquinone and hydroxyapatite/alginate scaffolds can improve osteogenic differentiation. [ 2 , 9 ]…”