Thyroglobulin (Tg) Testing Revisited: Tg Assays, TgAb Assays, and Correlation of Results With Clinical Outcomes

Netzel, Brian C.; Grebe, Stefan K.; Leon, B. Gisella Carranza; Castro, M. Regina; Clark, P. M. S.; Hoofnagle, Andrew N.; Spencer, Carole A.; Turcu, Adina F.; Algeciras‐Schimnich, Alicia

doi:10.1210/jc.2015-1967

Cited by 136 publications

(96 citation statements)

References 26 publications

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“…Interestingly, in his study, Spencer [84] showed that any detectable level of TgAb, although lower than the cutoff of positivity, has the potential to interfere with Tg measurement relative to its level. Recently, Netzel et al [85] In addition, some years ago, a paper [86] described FNA performed in two PTC patients with positive TgAb before and after rhTSH; detectable FNA-Tg levels were found only after rhTSH stimulation. The authors speculated that the Tg excess induced by rhTSH stimulation may 'saturate' all TgAb binding sites, explaining why Tg was only detectable after rhTSH stimulation in their patients.…”

Section: Samplingmentioning

confidence: 99%

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Measurement of thyroglobulin, calcitonin, and PTH in FNA washout fluids

Trimboli

D’Aurizio

Tozzoli

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Different imaging tools, circulating endocrine markers, and fine-needle aspiration (FNA) cytology are of great importance in the diagnosis and follow-up of different thyroid and parathyroid diseases. Sometimes, however, they are conflicting or inconclusive: interestingly, measuring endocrine markers (i.e. thyroglobulin, calcitonin, parathyroid hormone) in fluids from FNA proved to be a very useful complementary diagnostic tool in such cases. The determination of endocrine markers in fluids other than serum/ plasma has been developed in the last years. Although studies have reported overall satisfactory results, a good standardization of procedures has not yet been reached, and further efforts should be made in order to better define preanalytical, analytical, and post-analytical aspects. Here we reviewed critically the literature on the measurement of FNA endocrine markers, focusing on laboratory issues, such as preparation of the sample, choice of solution, and technical features of determination of these markers. Indeed, information for use of FNA-Tg, FNA-CT, and FNA-PTH in clinical practice was also provided.

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Section: Samplingmentioning

confidence: 99%

“…In particular, there is a large inter-method variability in results with IMAs for serum TgAb despite the introduction of the International Reference Preparation 65/093 (IRP 65/093) [88]. Further confusion may result from the use of different concentrations or cutoff values for defining 'positive' and 'negative' TgAb results [85].…”

Section: Samplingmentioning

confidence: 99%

Measurement of thyroglobulin, calcitonin, and PTH in FNA washout fluids

Trimboli

D’Aurizio

Tozzoli

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Earlier we listed the proteomic analysis of thyroglobulin as an area where MS can offer clinical benefit compared with immunoassay, due to the interference of autoantibodies. Unfortunately, MS does not provide all the needed answers in these cases because there are reports of image-confirmed disease recurrence that had undetectable concentrations of thyroglobulin by MS (38 ). A closer examination of the technology reveals that many variables need to be controlled to provide an accurate quantitative result.…”

Section: Challenges With Msmentioning

confidence: 99%

Effective Use of Mass Spectrometry in the Clinical Laboratory

2016

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BACKGROUND:Historically the success of mass spectrometry in the clinical laboratory has focused on drugs of abuse confirmations, newborn screening, and steroid analysis. Clinical applications of mass spectrometry continue to expand, and mass spectrometry is now being used in almost all areas of laboratory medicine.CONTENT: A brief background of the evolution of mass spectrometry in the clinical laboratory is provided with a discussion of future applications. Prominent examples of mass spectrometry are covered to illustrate how it has improved the practice of medicine and enabled physicians to provide better patient care. With increasing economic pressures and decreasing laboratory test reimbursement, mass spectrometry testing has been shown to provide cost-effective solutions. In addition to pointing out the numerous benefits, the challenges of implementing mass spectrometry in the clinical laboratory are also covered.

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“…Serum thyroglobulin is the widely used tumour marker in patients with DTC but its utility is frequently hampered by the presence of antithyroglobulin antibodies [45] . The analysis of thyroglobulin free DNA can bypass the antibodies and provide a non-invasive approach to assess tumour evolution [46] .…”

Section: Molecular Analysismentioning

confidence: 99%

Nodular Thyroid Disease and Thyroid Cancer in the Era of Precision Medicine

et al. 2017

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The management of thyroid nodules, one of the main clinical challenges in endocrine clinical practice, is usually straightforward. Although the most important concern is ruling out malignancy, there are grey areas where uncertainty is frequently present: the nodules labelled as indeterminate by cytology and the extent of therapy when thyroid cancer is diagnosed pathologically. There is evidence that the current available precision medicine tools (from all the “-omics” to molecular analysis, fine-tuning imaging or artificial intelligence) may help to fill present gaps in the future. We present here a commentary on some of the current challenges faced by endocrinologists in the field of thyroid nodules and cancer, and illustrate how precision medicine may improve their diagnostic and therapeutic capabilities in the future.

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Thyroglobulin (Tg) Testing Revisited: Tg Assays, TgAb Assays, and Correlation of Results With Clinical Outcomes

Cited by 136 publications

References 26 publications

Measurement of thyroglobulin, calcitonin, and PTH in FNA washout fluids

Measurement of thyroglobulin, calcitonin, and PTH in FNA washout fluids

Effective Use of Mass Spectrometry in the Clinical Laboratory

Nodular Thyroid Disease and Thyroid Cancer in the Era of Precision Medicine

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