Thyroid Transcription Factor 1 and Napsin A Double Stain: Utilizing Different Vendor Antibodies for Diagnosing Lung Adenocarcinoma

Johnson, Hunter; Cohen, Cynthia; Fatima, Nazneen; Duncan, David; Siddiqui, Momin T.

doi:10.1159/000339793

Cited by 18 publications

(21 citation statements)

References 40 publications

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“…Therefore, dependable IHC and molecular pathology results are of great value [14,15]. One major concern is whether this method can preserve the protein antigens and DNA for differential diagnosis and therapy.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Ultrasound-Processed Rapid Cell Blocks in the Cytopathologic Diagnosis of Cavity Fluids

Zhao¹,

Lin²,

Zhai

et al. 2014

Acta Cytologica

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Objective: The technique of conventional cell blocks is rather labor- and time-consuming. The purpose of this study was to generate a convenient and quick manual procedure using ultrasound processing which could be applied in most developing countries and to evaluate its efficacy in the cytopathologic diagnosis of cavity fluids. Study Design: We carried out a rapid cell block procedure using egg albumen as the pre-embedded adjuvant and using ultrasound to accelerate fixation, dehydration, clearing and waxing. The diagnostic efficacy was evaluated as compared with tissue blocks and liquid-based cytology tests (LCTs). Results: A total of 155 samples underwent rapid cell block detection, and 61 were diagnosed as malignancies. The method was able to produce high-quality formalin-fixed paraffin-embedded cell block sections and has similar diagnostic validity to the LCT. The immunohistochemistry and in situ hybridization staining patterns in rapid cell block sections were similar to those in their tissue block counterparts. Conclusions: The ultrasound-processed rapid cell block is a convenient and quick method for cytopathologic diagnosis. We consider it may serve as an effective adjuvant technique for most primary medical institutions.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Ultrasound-Processed Rapid Cell Blocks in the Cytopathologic Diagnosis of Cavity Fluids

Zhao¹,

Lin²,

Zhai

et al. 2014

Acta Cytologica

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“…One important point to consider is the occasional staining described in AdCA by p63, which was seen in a number of our cases as well but was lacking in the corresponding p40 staining. Johnson et al [17] have described a dual stain process utilizing TTF and napsin in the diagnosis of AdCA. This is helpful in providing a specific category diagnosis and in helping to preserve tissue in minimal biopsy specimens where additional ancillary testing (EGFR, Alk, etc.)…”

Section: Discussionmentioning

confidence: 99%

Utilization of p40 (ΔNp63) with p63 and Cytokeratin 5/6 Immunohistochemistry in Non-Small Cell Lung Carcinoma Fine-Needle Aspiration Biopsy

Collins¹,

Wang²,

Bernadt³

2013

Acta Cytologica

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Objective: Specific subclassification of pulmonary non-small cell carcinoma (NSCCA) is clinically necessary, and the aim of this study is to examine the utilization of p40 (ΔNp63) in fine-needle aspiration (FNA) biopsy for lung NSCCA. Study Design: Database files of the Washington University Medical Center were searched. Patients who underwent endobronchial ultrasound and CT FNA of a primary lung neoplasia were selected and immunohistochemistry (IHC) was performed. A panel of markers was utilized, including p40, p63, cytokeratin (CK) 5/6, thyroid transcription factor, and napsin. Results: One hundred patients were identified and comprised 38 squamous cell carcinomas (SCCA), 46 adenocarcinomas (AdCA), and 16 NSCCA. For SCCA, p40 was positive in 34/38 cases (89%) and negative in 4/38 cases (11%); p63 was positive in 33/38 cases (87%) and negative in 5/38 cases (13%); CK5/6 was positive in 38/38 cases. For AdCA cases, p40 was negative, p63 was positive in 2 cases (5%) and CK5/6 was negative in 43/46 cases (92%). Conclusion: For NSCCA, p40 had 89% sensitivity and 100% specificity compared to p63 with 86% sensitivity and 96% specificity and CK5/6 with 100% sensitivity and 96% specificity. In the evaluation of FNA biopsy for pulmonary NSCCA, p40 is a useful IHC marker for neoplastic subclassification, with better specificity in comparison to p63.

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“…Recently, several studies have addressed this issue by investigating the potential utility of the combination of several IHC markers as a single marker for NSCLC subclassification [25], [26], [27] and [28]. A dual marker combining TTF1 and Napsin A showed 74% sensitivity and 87% specificity [25], and 74% sensitivity and 88% to 96% specificity [26] in the identification of lung ADC using FNA material.…”

Section: Introductionmentioning

confidence: 99%

The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non–small cell lung cancer

Zhang

Sakowski

et al. 2014

Human Pathology

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Summary In lung cancer, targeted therapies depend on accurate histological subclassification of the tumor. The majority of lung cancers can be subclassified based on hematoxylin and eosin staining; however, classification may be difficult in small biopsies. In this study, we investigated the utility of a newly developed triple marker (combination of TTF1/Napsin A/p40) and compared the sensitivity and specificity of this novel marker with individual markers in the subclassification of non–small cell lung carcinomas. Lung cancer tissue microarrays were constructed using surgical resection material from the Johns Hopkins Hospital. They included 77 adenocarcinomas (ADCs), 77 squamous cell carcinomas (SqCCs), and 46 cases of metastatic lung ADCs. Immunostaining patterns of all markers were scored semi-quantitatively and compared. In ADCs, the sensitivity and specificity of the triple marker were 93.5% and 77.5%, respectively. The sensitivity and specificity of TTF1 and Napsin A were 85.7% and 75.0%, and 89.6% and 90.0%. In SqCCs, the sensitivity and specificity of the triple marker were 88.3% and 92.5%, while the p40, p63 and CK5/6 showed 80.5% and 90.0%; 93.5% and 80.0%; and 89.6% and 80.0%. In addition, the sensitivity and specificity of the triple marker in metastatic ADCs showed 71.7% and 73.5%, respectively. Our triple marker (combination of TTF1/Napsin A/p40) showed a similar sensitivity and specificity for the subclassification of NSCLC when compared to individual markers. Our study not only demonstrates a useful combination of immunomarkers but also optimally conserves tissue for molecular marker testing.

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Thyroid Transcription Factor 1 and Napsin A Double Stain: Utilizing Different Vendor Antibodies for Diagnosing Lung Adenocarcinoma

Cited by 18 publications

References 40 publications

Evaluation of Ultrasound-Processed Rapid Cell Blocks in the Cytopathologic Diagnosis of Cavity Fluids

Evaluation of Ultrasound-Processed Rapid Cell Blocks in the Cytopathologic Diagnosis of Cavity Fluids

Utilization of p40 (ΔNp63) with p63 and Cytokeratin 5/6 Immunohistochemistry in Non-Small Cell Lung Carcinoma Fine-Needle Aspiration Biopsy

The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non–small cell lung cancer

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Thyroid Transcription Factor 1 and Napsin A Double Stain: Utilizing Different Vendor Antibodies for Diagnosing Lung Adenocarcinoma

Cited by 18 publications

References 40 publications

Evaluation of Ultrasound-Processed Rapid Cell Blocks in the Cytopathologic Diagnosis of Cavity Fluids

Evaluation of Ultrasound-Processed Rapid Cell Blocks in the Cytopathologic Diagnosis of Cavity Fluids

Utilization of p40 (&#x0394;Np63) with p63 and Cytokeratin 5/6 Immunohistochemistry in Non-Small Cell Lung Carcinoma Fine-Needle Aspiration Biopsy

The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non–small cell lung cancer

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Utilization of p40 (ΔNp63) with p63 and Cytokeratin 5/6 Immunohistochemistry in Non-Small Cell Lung Carcinoma Fine-Needle Aspiration Biopsy