Thyrotropin activates both the cyclic AMP and the PIP2casades in CHO cells expressing the human cDNA of TSH receptor

Sande, Jacqueline Van; Raspé, Eric; Perret, Jason; Lejeune, Christophe; Maenhaut, Carine; Vassart, Gilbert; Dumont, Jacques Emile

doi:10.1016/0303-7207(90)90209-q

Cited by 188 publications

(88 citation statements)

References 10 publications

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“…TSH receptor activation leads to an increase in cAMP levels that ultimately induces Tg gene expression in vivo (36,43). In addition, it has been proposed that at higher concentrations TSH can also trigger a simultaneous rise in cytosolic free Ca 2ϩ (44).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Repressor DREAM Interacts with Thyroid Transcription Factor-1 and Regulates Thyroglobulin Gene Expression

Rivas

Mellström

Naranjo

et al. 2004

Journal of Biological Chemistry

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Tissue-specific gene expression depends on the interaction between tissue-specific and general transcription factors. DREAM is a Ca 2؉ -dependent transcriptional repressor widely expressed in the brain where it participates in nociception through its control of prodynorphin gene expression. In the periphery, DREAM is highly expressed in the thyroid gland, the immune system, and the reproductive organs. Here, we show that DREAM interacts with thyroid-specific transcription factor TTF-1 and regulates the expression of the thyroglobulin (Tg) gene. The mechanism also involves binding of DREAM to the thyroglobulin promoter and blockage of TTF-1-mediated transactivation. The TSH/cAMP pathway and Ca 2؉ signaling regulate DREAM-mediated transcriptional repression of the thyroglobulin gene. Furthermore, chromatin immunoprecipitation experiments in FRTL-5 cells confirmed that Tg is a bona fide target gene for DREAM transrepression in thyroid follicular cells.

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Section: Discussionmentioning

confidence: 99%

Transcriptional Repressor DREAM Interacts with Thyroid Transcription Factor-1 and Regulates Thyroglobulin Gene Expression

Rivas

Mellström

Naranjo

et al. 2004

Journal of Biological Chemistry

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“…In view of this possible function of TSH, we attempted to assess whether the pituitary TSH-R is able to stimulate cAMP production, which is known to be the most important TSH-R secondary messenger system in the thyrocyte (Van Sande et al 1990, Schaaf et al 1997. We used two cell lines, known to express TSH-R mRNA, and rat pituitary cells in primary culture to examine whether stimulation of the intrapituitary TSH-R is capable of altering cAMP levels.…”

Section: Discussionmentioning

confidence: 99%

Thyrotrophin receptor protein expression in normal and adenomatous human pituitary

Theodoropoulou¹,

Arzberger²,

Gruebler³

et al. 2000

Journal of Endocrinology

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Thyrotrophin (TSH) synthesis and secretion is under the positive control of thyrotrophin releasing hormone and under the negative control of the thyroid hormones. However, it is hypothesised that TSH has a direct effect on the regulation of its own synthesis through an intrapituitary loop mediated by pituitary TSH receptors (TSH-R). The aim of this investigation was to study the expression of TSH-R in normal human pituitary at mRNA and protein levels, and to compare the pattern of protein expression between different pituitary adenomas. Using RT-PCR we were able to detect TSH-R mRNA in the normal pituitary, and immunohistochemical studies showed TSH-R protein expression in distinct areas of the anterior pituitary. Double immunostaining with antibodies against each of the intrapituitary hormones and S100 revealed that TSH-R protein is present in thyrotrophs and folliculostellate cells. Examination of 58 pituitary adenomas, including two clinically active and two clinically inactive thyrotroph adenomas, revealed TSH-R immunopositivity in only the two clinically inactive thyrotroph adenomas.This study shows, for the first time, the presence of TSH-R protein in the normal anterior pituitary and in a subset of thyrotroph adenomas. The expression of TSH-R in the thyrotroph and folliculostellate cell subpopulations provides preliminary evidence of a role for TSH in autocrine and paracrine regulatory pathways within the anterior pituitary gland.

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“…Attempts to establish proliferating human thyroid epithelial cell cultures from normal donors have, until recently, been unsuccessful [13][14][15][16]. Long-term cultures of differentiated proliferating thyrocytes, which are not subject to 'spontaneous' transformation in vitro from normal individuals, have now been developed [17].…”

Section: Introductionmentioning

confidence: 99%

HLA-DMB expression by thyrocytes: indication of the antigen-processing and possible presenting capability of thyroid cells

Wu¹,

Biro²,

Mirakian³

et al. 1999

Clinical and Experimental Immunology

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SUMMARYExpression of HLA class II molecules on thyrocytes is a characteristic feature of autoimmune thyroid disease and may lead the thyroid cells to present autoantigens to CD4 þ T lymphocytes. Since HLA-DM is a critical molecule in class II-restricted antigen processing and presentation, we assessed the expression of HLA-DMB, -invariant chain (Ii), class II transactivator (CIITA) and DRA in an untransformed, pure thyrocyte strain HTV-59A. Here we report that both HLA-DMB mRNA and the protein are expressed in thyrocytes and that CIITA expression is enhanced by interferon-gamma (IFNg) treatment and occurs before DMB, Ii and DRA up-regulation, suggesting CIITA expression is a requirement for antigen processing in thyrocytes. These results indicate that thyrocytes are capable of antigen processing and possibly antigen presentation to T cells.

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Thyrotropin activates both the cyclic AMP and the PIP2casades in CHO cells expressing the human cDNA of TSH receptor

Cited by 188 publications

References 10 publications

Transcriptional Repressor DREAM Interacts with Thyroid Transcription Factor-1 and Regulates Thyroglobulin Gene Expression

Transcriptional Repressor DREAM Interacts with Thyroid Transcription Factor-1 and Regulates Thyroglobulin Gene Expression

Thyrotrophin receptor protein expression in normal and adenomatous human pituitary

HLA-DMB expression by thyrocytes: indication of the antigen-processing and possible presenting capability of thyroid cells

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