1975
DOI: 10.1016/s0021-9258(19)41094-6
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Thyrotropin receptors in thyroid plasma membranes. Characteristics of thyrotropin binding and solubilization of thyrotropin receptor activity by tryptic digestion

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Cited by 123 publications
(1 citation statement)
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“…Several assays have been used to measure the interaction between TSH and TSHR, but none have been ideal for identifying TSHR agonists via high-throughput screening (HTS). The traditional radiolabeled TSHR binding assay 5,6 is sensitive but does not measure direct activation of the receptor. Measurement of the amount of intracellular cAMP generated upon the activation of the TSHR more directly reflects activity at the receptor; however, cAMP radioimmunoassays 7 and enzyme-linked immunosorbent assays 8 require multiple assay steps and washes, making them nontransferable to HTS.…”
Section: Introductionmentioning
confidence: 99%
“…Several assays have been used to measure the interaction between TSH and TSHR, but none have been ideal for identifying TSHR agonists via high-throughput screening (HTS). The traditional radiolabeled TSHR binding assay 5,6 is sensitive but does not measure direct activation of the receptor. Measurement of the amount of intracellular cAMP generated upon the activation of the TSHR more directly reflects activity at the receptor; however, cAMP radioimmunoassays 7 and enzyme-linked immunosorbent assays 8 require multiple assay steps and washes, making them nontransferable to HTS.…”
Section: Introductionmentioning
confidence: 99%