Tibolone Improves the Degenerative Changes of Tongue Mucosa in Ovariectomized Female Rats

Eltokhey, Heba M.; Zahran, Dalia H.; Ezzat, Amel M.

doi:10.4172/2157-7099.1000130

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…The wide and clearly distinguished interlobular septa of the parotid and submandibular salivary glands in OVX are the result of atrophy of acinar and ductal cells due to estrogen deficiency 19,20) . Atrophy occurred due to vascular damage and permeability of blood vessels between lobes and similar results have been reported in the submandibular gland of elderly postmenopausal rats 21) , oral mucosal epithelium of OVX 22) , and the sublingual region of OVX 23) . Estrogen exhibits inhibitory effects on apoptosis in breast cancer cells, cardiac myocytes, endothelial cells, neuronal cells, and prostate cells 24) .…”

Section: Discussionsupporting

confidence: 84%

Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats

et al. 2022

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Background: Estrogen deficiency affects the structure and function of the salivary glands in women, leading to a decrease in salivary secretion and a change in the composition of saliva. Previous studies on changes in the salivary glands that cause estrogen deficiency have reported only partial results for the parotid and submandibular glands, and there are few comparative morphological studies of histological changes between the parotid and submandibular glands in ovariectomized rats (OVX) leading to estrogen deficiency. This study aimed to analyze the histopathological and histochemical changes in the parotid and submandibular salivary glands causing estrogen deficiency by using OVX, and to discuss the mechanism on these changes. Methods: The parotid and submandibular glands from sacrificed control and OVX groups were fixed with cold 4% paraformaldehyde in phosphate buffer (pH 7.2). The tissues were dehydrated using a series of graded ethyl alcohol and embedded in paraffin. For histopathological analysis, sections cut to a thickness of 6 to 7 µm were stained with hematoxylin and eosin (H&E). For histochemical analysis, Periodic acid-Schiff (PAS), Alcian blue (AB, pH 2.5), and PAS+AB (pH 2.5 and pH 1) staining was performed.Results: Histopathological analysis of OVX tissue showed that the parotid and submandibular salivary glands were broadly and clearly separated and divided into lobes. In OVX, acinar and ductal cells with condensed polymorphic or pyknotic nucleus, which are presumed to be characteristic of apoptotic cells, and degenerated cells with lipid deposition in cytoplasmic granules and ruptured membranes were increased. Histochemical analysis of OVX, confirmed an increase in the number and acidification of acinar secretory granules. Conclusion: Histopathological and histochemical changes and the effects of estrogen deficiency are more evident in the submandibular salivary gland than in the parotid gland.

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Section: Discussionsupporting

confidence: 84%

Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats

et al. 2022

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Epidermal growth factor attenuates lingual papillae lesions in a rat model of sialoadenectomy

Kattaia

Selim

et al. 2020

Tissue and Cell

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Mechanistic effect of human umbilical cord blood derived mesenchymal stem cells on the submandibular salivary gland in ovariectomized rats

Gouda

Khalifa

Shalaby

et al. 2018

Biochem. Cell Biol.

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We performed this study to understand the effect of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) on the submandibular gland after bilateral ovariectomy. For this, 21 adult female rats were distributed equally among 3 groups: the sham-operated group (SHAM); the ovariectomized group (OVX); and the OVX group that received repeated intravenous injections of the hUCB-MSCs (OVX + hUCB-MSCs). We used reverse transcription - PCR to analyze for the gene expression of AQPs 3, 4, 5, and BMP-6. The cellular localization and expression of human CD105, human CD34, proliferating nuclear antigen (PCNA), single-stranded DNA (ss-DNA), caspase 3, AQP1, and α smooth muscle actin (α-SMA) were determined immunohistochemically. In the OVX group, a significant decrease in the gene expression of AQP3, AQP4, and BMP6, as well as the acinar area % was detected, while area % of granular convoluted tubules (GCTs) showed a significant increase. A significant decrease in area % staining positively for AQP1 and α-SMA was noted. An obvious improvement in the structure of the submandibular gland was demonstrated in the group injected with hUCB-MSCs, as well as a significant increase in the gene expression of AQP3, AQP4, and BMP6. The acinar and GCT area %, as well as the different measured markers, were relatively normal. This demonstrates that E2-deficiency induces structural changes to the submandibular gland. Moreover, a definite amelioration of the structure and function of the submandibular gland was detected after the administration of hUCB-MSCs.

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Tibolone Improves the Degenerative Changes of Tongue Mucosa in Ovariectomized Female Rats

Cited by 3 publications

References 14 publications

Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats

Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats

Epidermal growth factor attenuates lingual papillae lesions in a rat model of sialoadenectomy

Mechanistic effect of human umbilical cord blood derived mesenchymal stem cells on the submandibular salivary gland in ovariectomized rats

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