Tick-borne agents in domesticated and stray cats from the city of Campo Grande, state of Mato Grosso do Sul, midwestern Brazil

André, Marcos Rogério; Herrera, Heitor Miraglia; Fernandes, Sérgio Rodrigo; Sousa, Keyla Cartens Marques de; Gonçalves, Luiz Ricardo; Domingos, Iara Helena; Macedo, Gabriel Carvalho de; Machado, Rosangela Zacarias

doi:10.1016/j.ttbdis.2015.07.004

Cited by 65 publications

(76 citation statements)

References 53 publications

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“…Ehrlichia genotypes closely related to E. canis have already been detected in wild carnivores , wild birds and domestic cats (André et al, 2015) in Brazil, and rodents in Korea (Kim et al, 2006). In addition, Ehrlichia minasensis, a species closely related to Ehrlichia canis that has been identified infecting Rhipicephalus microplus ticks and cattle in Brazil (Carvalho et al, 2016;Cabezas-Cruz, et al;2016), also showed phylogenetic relatedness with the sequences obtained in the present study, but lower when compared to Ehrlichia canis.…”

Section: Discussionsupporting

confidence: 74%

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Benevenute

Dumler

Ogrzewalska

et al. 2017

Ticks and Tick-borne Diseases

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New genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5' nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n=60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas.

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Section: Discussionsupporting

confidence: 74%

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Benevenute

Dumler

Ogrzewalska

et al. 2017

Ticks and Tick-borne Diseases

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“…The pre-established PCR protocols targeting genes other than 16S rRNA have shown low accuracy in the identification of new Anaplasmataceae genotypes in Brazil (MURPHY et al, 1998;MASSUNG et al, 1998;KOCAN et al, 2000). This pattern of positive results to Anaplasmataceae 16S rRNA gene in PCR assays and negative results to other target genes was already described in previous studies that aimed at detecting Anaplasmataceae in domestic and wild animals in Brazil (ANDRÉ et al, 2012, 2015SACCHI et al, 2012). Blood samples showing low bacteremia, and an inadequate PCR protocol for a variant of Anaplasma sp.…”

Section: Introductionmentioning

confidence: 84%

Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

Mongruel

Benevenute

Ikeda

et al. 2017

Rev. Bras. Parasitol. Vet.

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Wild animals play an important role in carrying vectors that may potentially transmit pathogens. Several reports highlighted the participation of wild animals on the Anaplasma phagocytophilum cycle, including as hosts of the agent. The aim of this study was to report the molecular detection of an agent phylogenetically related to A. phagocytophilum isolated from a wild bird in the Midwest of the state of Paraná, Brazil. Fifteen blood samples were collected from eleven different bird species in the Guarapuava region. One sample collected from a Penelope obscura bird was positive in nested PCR targeting the 16S rRNA gene of Anaplasma spp. The phylogenetic tree based on the Maximum Likelihood analysis showed that the sequence obtained was placed in the same clade with A. phagocytophilum isolated from domestic cats in Brazil. The present study reports the first molecular detection of a phylogenetically related A. phagocytophilum bacterium in a bird from Paraná State.

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“…Recently, there has been a surge of Ehrlichia spp. as a significant vector-borne agent in cats in some areas of the world, such as the United States of America (BREITSCHWERDT et al, 2002), Brazil (BRAGA et al, 2012, 2013ANDRÉ et al, 2015;, Portugal (MAIA et al, 2014), and Angola (OLIVEIRA et al, 2018).…”

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confidence: 99%

Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

Guimarães

Raimundo

Rodrigues

et al. 2019

Rev. Bras. Parasitol. Vet.

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Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms. ResumoA erliquiose é causada por agentes pertencentes ao gênero Ehrlichia. Apesar dos frequentes relatos de detecção sorológica e molecular de E. canis em cães no Brasil, existem poucos dados sobre a erliquiose em gatos brasileiros. Este estudo teve como objetivo investigar a ocorrência de Ehrlichia spp. em gatos domésticos do Grande Rio de Janeiro e avaliar as alterações hematológicas associadas a essa infecção rickettsial. Procuramos anticorpos IgG anti-E. canis em amostras de sangue de 216 gatos por Reação de Imunofluorescência Indireta (RIFI). Além disso, foram realizados ensaios de nested PCR (nPCR) e PCR em tempo real (qPCR) para detecção dos genes E. canis-16S rRNA e dsb, respectivamente. Cinquenta e sete (26,4%) gatos foram soropositivos para Ehrlichia spp. pela RIFI. Fragmentos do gene rRNA de Ehrlichia spp.-16S foram detectados em 3 gatos (1,4%) por ensaios de nPCR. Embora as sequências 16S rRNA obtidas tenham 99 a 100% de identidade com E. canis, os gatos foram negativos nos ensaios de qPCR. Anemia, trombocitopenia, leucocitose, desvio nuclear neutrofílico à esquerda e hiperproteinemia foram observados. Anemia foi estatisticamente associada à soropositividade para E. canis e filhotes apresentaram menores taxas de positividade (p <0,05). Este estudo demonstra que Ehrlichia spp. ocorrem em gatos domésticos da Grande Rio de Janeiro. Outros estudos envolvendo o isolamento por cultura são necessários para caracterizar com mais precisão esses organismos.Palavras-chave: Felino, nested PCR, RIFI, hematologia, erliquiose.

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Tick-borne agents in domesticated and stray cats from the city of Campo Grande, state of Mato Grosso do Sul, midwestern Brazil

Cited by 65 publications

References 53 publications

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

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