Tight Junctions: Molecular Architecture and Function

Aijaz, Saima; Balda, María S.; Matter, Karl

doi:10.1016/s0074-7696(06)48005-0

Cited by 283 publications

(223 citation statements)

References 232 publications

Supporting

Mentioning

220

Contrasting

Unclassified

Order By: Relevance

“…It regulates the paracellular diffusion of small hydrophilic molecules, has been linked to the formation of the intramembrane diffusion barrier and regulates the transepithelial migration of neutrophils (Aijaz et al, 2006). Either one of its N-and C-terminal cytosolic domains is sufficient to anchor the protein in the junction and to ensure a continuous junctional distribution.…”

Section: Occludinmentioning

confidence: 99%

“…The molecular mechanisms that are responsible for selective ion permeability and for diffusion of small hydrophilic molecules are distinct, as many manipulations and regulatory mechanisms specifically affect only one of the two processes, or downregulate one while activating the other (Aijaz et al, 2006). For example, the overexpression of occludin or certain occludin mutants in cultured epithelial cells stimulates the paracellular diffusion of small hydrophilic molecules but increases transepithelial electrical resistance (a measure of transepithelial ion permeability) (Aijaz et al, 2006).…”

Section: Paracellular Permeabilitymentioning

confidence: 99%

“…However, the process is regulated by RhoA signalling and seems to require actinomyosin-driven processes (Aijaz et al, 2006;McKenzie and Ridley, 2007;Nusrat et al, 2000;Utech et al, 2006). It is therefore possible that dynamic rearrangements of intramembrane strands lead to paracellular diffusion.…”

Section: Paracellular Permeabilitymentioning

confidence: 99%

“…It is therefore possible that dynamic rearrangements of intramembrane strands lead to paracellular diffusion. Occludin has been linked to the regulation of paracellular permeability of small hydrophilic molecules across cultured epithelial monolayers Schneeberger and Lynch, 2004); it is thought that this regulatory mechanism involves phosphorylation events as well as the actin cytoskeleton because the C-terminal domain of occludin binds to protein kinases and lipid kinases, as well as to actin filaments and cytoskeletal linkers (Aijaz et al, 2006;Schneeberger and Lynch, 2004). Strikingly, live-cell-imaging experiments using GFP-tagged occludin have recently suggested that occludin diffuses within the junction, suggesting that occludin dynamics might contribute to paracellular diffusion (Shen et al, 2008).…”

Section: Paracellular Permeabilitymentioning

confidence: 99%

See 3 more Smart Citations

Tight junctions at a glance

Balda

Matter

2008

Journal of Cell Science

Self Cite

214

168

View full text Add to dashboard Cite

Section: Occludinmentioning

confidence: 99%

Section: Paracellular Permeabilitymentioning

confidence: 99%

Section: Paracellular Permeabilitymentioning

confidence: 99%

Section: Paracellular Permeabilitymentioning

confidence: 99%

See 2 more Smart Citations

Tight junctions at a glance

Balda

Matter

2008

Journal of Cell Science

Self Cite

214

168

View full text Add to dashboard Cite

“…The cytoplasmic tail of VE-cadherin binds b-catenin or plakoglobin, which in turn are linked to a-catenin that finally anchors the complex to the actin cytoskeleton. Tight junctions seal the paracellular space between two adjacent cells and establish cell polarity [10]. They are composed of several type 3 transmembrane proteins including occludin and members of the claudin family, presence of which induces tight junctions [11].…”

Section: Introductionmentioning

confidence: 99%

Distinct molecular composition of blood and lymphatic vascular endothelial cell junctions establishes specific functional barriers within the peripheral lymph node

et al. 2008

View full text Add to dashboard Cite

Lymph nodes are strategically localized at the interfaces between the blood and lymphatic vascular system, delivering immune cells and antigens to the lymph node. As cellular junctions of endothelial cells actively regulate vascular permeability and cell traffic, we have investigated their molecular composition by performing an extensive immunofluorescence study for adherens and tight junction molecules, including vascular endothelium (VE)-cadherin, the vascular claudins 1, 3, 5 and 12, occludin, members of the junctional adhesion molecule family plus endothelial cell-selective adhesion molecule (ESAM)-1, platelet endothelial cell adhesion molecule-1, ZO-1 and ZO-2. We found that junctions of high endothelial venules (HEV), which serve as entry site for naive lymphocytes, are unique due to their lack of the endothelial cell-specific claudin-5. LYVE-1 + sinus-lining endothelial cells form a diffusion barrier for soluble molecules that arrive at the afferent lymph and use claudin-5 and ESAM-1 to establish characteristic tight junctions. Analysis of the spatial relationship between the different vascular compartments revealed that HEV extend beyond the paracortex into the medullary sinuses, where they are protected from direct contact with the lymph by sinus-lining endothelial cells. The specific molecular architecture of cellular junctions present in blood and lymphatic vessel endothelium in peripheral lymph nodes establishes distinct barriers controlling the distribution of antigens and immune cells within this tissue.

show abstract

Cellular Tight Junctions as Mediators of Adverse Effects

Sambuy

2009

General, Applied and Systems Toxicology

View full text Add to dashboard Cite

Tight junctions are highly complicated and finely regulated structures that provide for the barrier and exchange functions of epithelia and endothelia at the interface between the internal organs and the external environment, and are therefore frequently exposed to noxious stimuli. Recent evidence points to epithelial and endothelial barrier dysfunction, resulting from changes in structure of the tight junctions, as an important early toxic event, preceding more severe damage to the cells. Increased tight junction permeability has also been implicated in the pathogenesis of several multifactorial diseases, frequently involving an exaggerated inflammatory response. Some examples of tight junction alterations occurring after exposure to toxic stimuli, including ethanol, heavy metals and oxidative stress, will be discussed in more detail. In addition, the implications of barrier dysfunction in pathological conditions including hereditary disorders of tight junction proteins, cancers, microbial infections, inflammatory bowel disease, multiple sclerosis and coeliac disease will be presented.

show abstract

Tight Junctions: Molecular Architecture and Function

Cited by 283 publications

References 232 publications

Tight junctions at a glance

Tight junctions at a glance

Distinct molecular composition of blood and lymphatic vascular endothelial cell junctions establishes specific functional barriers within the peripheral lymph node

Cellular Tight Junctions as Mediators of Adverse Effects

Contact Info

Product

Resources

About