Time-course and intensity-based classifications of oxidative stresses and their potential application in biomedical, comparative and environmental research

Lushchak, Volodymyr I.

doi:10.1080/13510002.2015.1126940

Cited by 13 publications

(9 citation statements)

References 93 publications

(127 reference statements)

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“…Hydrogen peroxide refers to reactive forms of oxygen and, with increased formation in the cell, causes oxidative stress. This substance is neutralized with the participation of antioxidative enzymes in the cytoplasm of cells and some organelles (Asatiani et al, 2011;Lushchak et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…In chemical kinetics, the food industry, in certain issues of pathology, toxicology, radiobiology, biochemistry, biophysics, etc., there have been scattered studies on lipid peroxidation, but any of them taken separately, as it were, is a special case secondary in the scale of each of the listed sciences. But, with the accumulation of facts, it became more and more obvious that the study of the free radical chain oxidation of unsaturated fatty acids in a cell is in itself an important scientific problem, located at the intersection of a number of related disciplines (Lushchak, 2016;. First of all, the problem of LPP in biological membranes, as well as membrane biology in general, lies on the boundary between biochemistry and biophysics.…”

Section: Introductionmentioning

confidence: 99%

“…Biochemical methods for extraction of subcellular particles, enzyme control, as well as analysis of peroxidation products, are found to be an important addition to biophysical methods for studying free radical processes, among which in this case the most fruitful was the measurement of chemiluminescence accompanying these processes. The biochemical concept of metabolism, regulated by a number of factors, including enzymes, is supplemented by biophysical categories of free radical reactions in the biomolecular structure of biological membranes (Lushchak, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…While the investigation of kinetics is carried out on the example of hydrocarbons, and the study of pathology is carried out at the organism level, there is no real connection between these two directions. But, with the transition of research to the level of solutions and suspensions of lipids, biological membranes or organelles, kinetics and pathology become the cause and effect of the same molecular events (Lushchak, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The state of antioxidant protection system in cows under the influence of heavy metals

Slivinska

Shcherbatyy

Lukashchuk

et al. 2020

Regul. Mech. Biosyst.

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A highly relevant problem of modern veterinary science is the study of features and mechanisms of combined action of the most common heavy metals – cadmium and plumbum and their influence on the body of humans and animals in the regions of Ukraine under technogenic pollution. The purpose of the work was to study the influence of heavy metals on the state of the antioxidant protection system of cows, in particular on the content of lipid peroxidation products (malonic dialdehyde, lipid hydroperoxides and diene conjugates), and activity of antioxidant enzymes (glutathione peroxidase and superoxide dismutase), depending on the distance to the heaps of mines in the coal basin. The study objects were cows of black-and-white breed at the age of 3–7 years. It was established that this parameter in the place with the highest concentration of diene conjugates in the blood of cows was by 25.8 % higher compared to the place of low concentration and 12.1 % higher compared to the place with medium concentration. In the place with the highest content of lipid hydroperoxides in the blood of cows the parameter was 23.7 % higher compared to the cows from the place with the low content. The concentration of lipid hydroperoxides in the blood of cows from the place with the medium content was 16.7% higher compared to the cows from the place with the low content. The parameter from the place with the lowest content of lipid hydroperoxides in the blood of cows was 12.1% lower compared to the place with the highest content. The level of malonic dialdehyde in the blood of cows from the technogenic pollution zone in the place with the largest amount was higher by 36.2; 34.0 and 18.8 % – compared to places with medium and low levels, respectively. The activity of superoxide dismutase in the blood of cows in the place with its highest activity was 0.284 ± 0.0099 % block. reac/g Hb, and in the place with the lowest activity – 0.23 ± 0.0051 % block. reac/g Hb. The activity of glutathione peroxidase in the blood of cows in farms of the technogenic pollution zone depended on the distance to the mine. These researches will further develop effective methods of treating cows under the influence of heavy metals, in particular regarding the antioxidant system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The state of antioxidant protection system in cows under the influence of heavy metals

Slivinska

Shcherbatyy

Lukashchuk

et al. 2020

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

show abstract

“…These enzymes have been widely used, namely peroxidase, which is used as a diagnostic reagent or marker enzyme in enzyme-linked immunosorbent assay, as a preservative in the food industry; catalase as a component of sorbent for stabilization of blood products, for cold sterilization of milk, cheese, eggs, in processes of organic synthesis, polymerization of rubber, etc. (Mhamdi et al, 2010;Lushchak, 2016).…”

Section: Introductionmentioning

confidence: 99%

Modification of isolation methods and physico-biochemical properties of preparations of fungal oxidoreductases

Fedotov

Usikova

2020

Regul. Mech. Biosyst.

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The results of the modification of methods for producing enzymatic preparations (EP) of peroxidases and catalases of extra- and intracellular finding from fungal cultures are presented. Strains of Flammulina velutipes F-vv, Lentinula edodes 523 and Pleurotus ostreatus P-01 were used as producers of oxidoreductases. The producers were cultured in glucose-peptone medium, modified for each strain. Protein fractionation was carried out by leaching with ammonium sulfate at a saturation of 40–70% for peroxidases and 80% for catalases. The obtained solutions of protein fractions were additionally subjected to purification by dialysis, gel filtration on Molselect G-50 and G-75 granules, and also freeze drying. The yield of enzymatic preparations per unit mass of mycelium and the volume of culture fluid were calculated. The individual characteristics of EP – enzymatic activity, the mass percentage of protein and associated amino acids, the ratio of the latter in groups depending on the nature of the radicals (amphotericity) of protein molecules are established. It was proved that the amino acid content in the proteins of fungal EP catalases and peroxidases indicates their acidic nature and this is confirmed by the pH values of aqueous solutions. Examination and toxicity testing of enzymatic preparations were carried out in certified laboratories, which confirmed their characteristics and compliance with safety requirements. The therapeutic properties of amino acids that are part of proteins or are in a free state in enzyme preparations are analyzed. In this way, the methods have been developed for producing enzymatic preparations of peroxidases and catalases of extra- and intracellular location, which allow new antioxidant enzymes with individual properties to be obtained, and, as a result, bring prospects for use in various industries and scientific research.

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Using ecotoxicology for conservation: From biomarkers to modeling

2022

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Time-course and intensity-based classifications of oxidative stresses and their potential application in biomedical, comparative and environmental research

Abstract: Most applications of the classifications proposed are theoretical and applied studies where oxidative stress takes place.

Cited by 13 publications

References 93 publications

The state of antioxidant protection system in cows under the influence of heavy metals

The state of antioxidant protection system in cows under the influence of heavy metals

Modification of isolation methods and physico-biochemical properties of preparations of fungal oxidoreductases

Using ecotoxicology for conservation: From biomarkers to modeling

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