Time-Course Effect of Electrical Stimulation on Nerve Regeneration of Diabetic Rats

Yao

et al. 2017

Sci Rep

Self Cite

Recent studies describe taxol as a candidate treatment for promoting central nerve regeneration. However, taxol has serious side effects including peripheral neurotoxicity, and little information is known about the effect of taxol on peripheral nerve regeneration. We investigated the effects of taxol on regeneration in a rat sciatic nerve transection model. Rats were divided into four groups (n = 10): normal saline (i.p.) as the control, Cremophor EL vehicle, and 2 or 6 mg/kg of taxol in the Cremophor EL solution (four times in day-2, 4, 6, and 8), respectively. We evaluated neuronal electrophysiology, animal behaviour, neuronal connectivity, macrophage infiltration, location and expression levels of calcitonin gene-related peptide (CGRP), and expression levels of both nerve growth factors and immunoregulatory factors. In the high-dose taxol group (6 mg/kg), neuronal electrophysiological function was significantly impaired. Licking latencies were significantly changed while motor coordination was unaffected. Neuronal connectivity, macrophage density, and expression levels of CGRP was dramatically reduced. Expression levels of nerve growth factors and immunoregulatory factors was also reduced, while it was increased in the low-dose taxol group (2 mg/kg). These results indicate that taxol can modulate local inflammatory conditions, impair nerve regeneration, and impede recovery of a severe peripheral nerve injury.

Section: Methodsmentioning

confidence: 99%

Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model

Yao

et al. 2017

Sci Rep

Self Cite

“…The RSC96 and BMMSC cells were inoculated on the surface of the material for co culture, and electrical stimulation was applied with 100 mV for 2 hours each 24 hours. 20,41,42 Aer the cell inoculation, the regular electrical stimulation was carried out every day from the third day. The effects of electrical stimulation on proliferation and differentiation of RSC96 cells and BMMSC cells were observed respectively on second day and forth day ( Fig.…”

Section: 8mentioning

confidence: 99%

A conductive sodium alginate and carboxymethyl chitosan hydrogel doped with polypyrrole for peripheral nerve regeneration

et al. 2018

RSC Adv.

134

“…Additionally, electrical stimulation could improve circulation to accelerate regenerative processes and ameliorate nerve functions [17]. Our group has also successfully demonstrated the beneficial effects of percutaneous electrical stimulation on long sciatic nerve defects in normal as well as diabetic rats [17][18][19][20]. However, the opposite results of electric fields on regenerating nerves have been reported, too [21,22].…”

Section: Introductionmentioning

confidence: 89%

“…Macrophages (CD68 + ) in the distal regions of the regenerated nerves were studied in immunostaining images under an SP2/SP8X microscope (Leica, Wetzlar, Germany). An image analyzer system (Image-Pro Lite, Media Cybernetics, Rockville, MD, USA) was used to measure the ratio of positive CGRP-immunoreactive areas in the dorsal horn ipsilateral to the injury and the number of neural components [20].…”

Section: Histological Analyses and Image Assaymentioning

confidence: 99%

Effects of Electrical Stimulation on Peripheral Nerve Regeneration in a Silicone Rubber Conduit in Taxol-Treated Rats

et al. 2020

Self Cite

Taxol, a type of antimitotic agent, could modulate local inflammatory conditions in peripheral nerves, which may impair their regeneration and recovery when injured. This study provided in vivo trials of silicone rubber chambers to bridge a long 10 mm sciatic nerve defect in taxol-treated rats. It was aimed to determine the effects of electrical stimulation at various frequencies on regeneration of the sciatic nerves in the bridging conduits. Taxol-treated rats were divided into four groups (n = 10/group): sham control (no current delivered from the stimulator); and electrical stimulation (3 times/week for 3 weeks at 2, 20, and 200 Hz with 1 mA current intensity). Neuronal electrophysiology, animal behavior, neuronal connectivity, macrophage infiltration, calcitonin gene-related peptide (CGRP) expression levels, and morphological observations were evaluated. At the end of 4 weeks, animals in the low- (2 Hz) and medium-frequency (20 Hz) groups had dramatic higher rates of successful regeneration (90% and 80%) across the wide gap as compared to the groups of sham and high-frequency (200 Hz) (60% and 50%). In addition, the 2 Hz group had significantly larger amplitudes and evoked muscle action potentials compared to the sham and the 200 Hz group, respectively (P < 0.05). Heat, cold plate licking latencies, motor coordination, and neuronal connectivity were unaffected by the electrical stimulation. Macrophage density, CGRP expression level, and axon number were all significantly increased in the 20 Hz group compared to the sham group (P < 0.05). This study suggested that low- (2 Hz) to medium-frequency (20 Hz) electrical stimulation could ameliorate local inflammatory conditions to augment recovery of regenerating nerves by accelerating their regrowth and improving electrophysiological function in taxol-treated peripheral nerve injury repaired with the silicone rubber conduit.