Time‐dependent changes in extra‐domain A‐fibronectin concentration and relative amounts of fibronectin‐fibrin complexes in plasma of patients with peripheral arterial disease after endovascular revascularisation

Abstract

Fibronectin (FN) may be involved in time- and stage-dependent and inter-related controlled processes of inflammation, coagulation, and wound healing accompanying peripheral arterial disease (PAD). In the present study, FN and FN-containing extra-domain A (EDA-FN), macromolecular FN-fibrin complexes, and FN monomer were analysed in the plasma of 142 PAD patients, including 37 patients with restenosis, for 37 months after revascularisation. FN concentration increased significantly in the plasma of PAD patients within 7 to 12 months after revascularisation, whereas the high concentration of EDA-FN was maintained up to 24 months, significantly higher in the group 7 to 12 months after revascularisation with recurrence of stenosis and lower in the PAD groups 1 to 3 months and 4 to 6 months after revascularisation with comorbid diabetes and ulceration, respectively. The relative amounts of FN-fibrin complexes up to 1600 kDa and FN monomer were significantly higher, within intervals of 4 to 24 months and 4 to 6 months after revascularisation, respectively. Moreover, the relative amounts of 750 to 1600 kDa FN-fibrin complexes within 13 to 24 months after revascularisation were higher in comparison with those in the group without restenosis. In conclusion, high levels of EDA-FN and FN-fibrin complexes could have potential diagnostic value in the management of PAD patients after revascularisation, predicting restenosis risk.

Cellular fibronectin promotes deep vein thrombosis in diet‐induced obese mice

“…Increased plasma Fn‐EDA levels are reported in patients with cardiovascular disorders 10,23 and in overweight, diabetic patients 9 . We first determined the Fn‐EDA levels in the plasma of VTE patients using ELISA.…”

Changes of plasma fibronectin and fibronectin-fibrin complexes in dams of stillborn dairy calves

“…A high fibrinogen concentration is also reported at the time of delivery and placental expulsion [ 31 ]. Activation of the coagulation cascade by an inflammatory agent(s) leads to formation of fibrin from fibrinogen, which easily forms with pFN macromolecular complexes, which has been reported to occur in plasma of human patients who suffer from some inflammatory diseases [ 16 – 18 , 32 ] and in cynomolgus monkey plasma after treatment with toxic high doses of recombinant clotting factor XIII [ 33 ].…”

“…Transglutaminase XIIIa catalyzes covalent cross-linking of FN to the carboxyl αC-domain of fibrin [11]. The crosslinked FN-fibrin polymers form a lattice of provisional matrix in damaged tissue [6,10,[12][13][14], and moreover they may occur in biological fluids [15][16][17][18]. The circulating soluble FN-fibrin complexes with molecular masses from around 750 kDa to 2100 kDa were revealed by Kątnik-Prastowska's group in puerperal plasma samples in human patients with some diseases, especially when inflammation was present, but rarely in healthy individuals [15][16][17][18].…”

Role of Infection and Immunity in Bovine Perinatal Mortality: Part 2. Fetomaternal Response to Infection and Novel Diagnostic Perspectives

“…Control dams that delivered healthy calves had complexes of 750 kDa to 1000 kDa. In humans circulating soluble FNfibrin complexes with molecular masses from around 750 kDa to 2100 kDa were present in puerperal plasma samples in patients with some diseases, especially when inflammation was present, but rarely in healthy individuals [112][113][114]. For these changes detected in dams to be used as a marker of stillbirth in cattle requires further research.…”