Time-dependent impairments in learning and memory in Streptozotocin-induced hyperglycemic rats

Ahmed, Ayaz; Zeng, Gaofeng; Jiang, Dejiang; Lin, Hua; Azhar, Mudassar; Farooq, Ahsana Dar; Choudhary, Muhammad Iqbal; Liu, Xinmin; Wang, Qiong

doi:10.1007/s11011-019-00448-7

Cited by 21 publications

(16 citation statements)

References 63 publications

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“…MWM test was a classical behavioral method for evaluating the reference, spatial, and working memory of rodents (Meiri and Rosenblu, 1998;Yongliang et al, 2018;Ahmed et al, 2019). It was reported that SAMP8 mice showed spatial memory impairment from 6 months of age (Qin et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Protective Effects of Ginsenosides Rb1 and Rg1 on Improving Cognitive Deficits in SAMP8 Mice Based on Anti-Neuroinflammation Mechanism

Yang

Huang

et al. 2020

Front. Pharmacol.

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This present study was designed to investigate the different effects of ginsenosides Rb1 and Rg1 on improving cognitive deficits in 4-month-old SAMP8 mice. Mice were divided into six groups, including the SAMP8 group, the SAMP8 + Donepezil (1.6 mg/kg) group, the SAMP8 + Rb1 (30 and 60 µmol/kg), and SAMP8 + Rg1 (30 and 60 µmol/kg) groups. SAMR1 mice of the same age were used as the control group. Ginsenosides and donepezil were administrated orally to animals for 8 weeks, then the learning and memory ability of mice were measured by using Morris water maze (MWM) test, object recognition test and passive avoidance experiments. The possible mechanisms were studied including the anti-glial inflammation of Rb1 and Rg1 using HE staining, immunohistochemistry and western blot experiments. Results revealed that Rb1 and Rg1 treatment significantly improved the discrimination index of SAMP8 mice in the object recognition test. Rb1 (60 µmol/kg) and Rg1 (30, 60 µmol/kg) could significantly shorten the escape latency in the acquisition test of the MWM test in SAMP8 mice. Furthermore, Rb1 and Rg1 treatments effectively reduced the number of errors in the passive avoidance task in SAMP8 mice. Western blot experiments revealed that Rb1 showed higher effect than Rg1 in decreasing protein expression levels of ASC, caspase-1 and Ab in the hippocampus of SAMP8 mice, while Rg1 was more effective than Rb1 in decreasing the protein levels of iNOS. In addition, although Rb1 and Rg1 treatments showed significant protective effects in repairing neuronal cells loss and inhibiting the activation of astrocyte and microglia in hippocampus of SAMP8 mice, Rb1 was more effective than Rg1. These results suggest that Rb1 and Rg1 could improve the cognitive impairment in SAMP8 mice, and they have different mechanisms for the treatment of Alzheimer's disease.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Protective Effects of Ginsenosides Rb1 and Rg1 on Improving Cognitive Deficits in SAMP8 Mice Based on Anti-Neuroinflammation Mechanism

Yang

Huang

et al. 2020

Front. Pharmacol.

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“…Learning and memory (both reference and working) can be impaired in diabetes (Ahmed et al, 2019; Saedi et al, 2016). Reference memory is referred as long‐term information storage which remains constant with the passing time, whereas working memory is short‐term and can be manipulated by the situation (Lee, Tucci, Sovrano, & Vallortigara, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The prepared homogenate was used to estimate the protein expression levels of CREB/pCREB, AKT/pAKT. Proteins were electrophoresed by using SDS‐10% PAGE, transferred to PVDF membrane, and protein expression was determined by using antibodies for CREB/pCREB, AKT/pAKT, and actin (1:1,000; Abcam), respectively, as described earlier (Ahmed et al, 2019; Xu, Wang, et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

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Jiawei Shengmai San herbal formula ameliorates diabetic associate cognitive decline by modulatingAKTandCREBin rats

Ahmed

Zeng

Azhar

et al. 2020

Phytotherapy Research

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Memory loss is a complication of diabetes which requires new approaches to its treatment. Shengmai San (SMS) is a famous traditional Chinese formula containing Panax ginseng, Ophiopogon japonicas, and Schisandra chinensis, whereas Radix puerariae has many reported pharmacological uses. In this study the combination, as Jiawei SMS (J-SMS) was screened for its ability to reverse diabetes-associated cognitive decline in rats. This was assessed behaviorally in diabetic rats (Streptozotocin, 45 mg/kg), with biochemical and western blot analysis (Akt and CREB). Diabetic rats showed fasting blood glucose (FBG) in the range of 13-15 mM throughout the study. J-SMS (0.5, 1.5, 4.5 g/kg) treatment significantly improved learning and memory deficit among diabetic rats as evidenced by preference for novel object, reduced escape latency and increased number of platform crossings (p < .05) in the NORT and MWM tests. Treatment with J-SMS also significantly improved the histopathological changes in the diabetic brain and increased the protein expression of AKT and CREB, required for proper memory function (p < .01). This study highlighted that J-SMS can reverse reference and working memory deficit among diabetic rats by modulating AKT and CREB proteins activation. Thus, J-SMS formulation might be possible candidate for further development.

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“…These complications typically include microvascular (retinopathy, neuropathy, and nephropathy) and macrovascular (cardiovascular and cerebrovascular) diseases [4]. Diabetes affects the central nervous system (CNS) associated with cognition, especially memory and learning; moreover, it increases the risk of developing dementia by up to 60% [5]. Hyperglycemia enhances the formation of reactive oxygen species (ROS) through glucose autoxidation, polyol pathways, and non-enzymatic glycation of proteins.…”

Section: Introductionmentioning

confidence: 99%

Memory and Learning Improvement by Resveratrol and Probiotics via the ‎Gut-Brain Axis and Antioxidant Activity in Diabetic Rats

Mashayekhi

Khodadadi

Mirzaei

et al. 2021

ajnpp

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Background and Objectives: Diabetes affects the central nervous system associated with cognition, especially memory and learning. The present study aimed to investigate the effects of probiotics (living microorganisms that provide health benefits) and resveratrol (a polyphenol with potential antioxidant activity) combination on oxidative stress, glucagon-like peptide-1 (GLP-1), memory, and learning in diabetic rats. Materials and Methods: Male Wistar rats were randomly divided into five groups (six animals per group) of control, diabetic, probiotic-treated diabetic (50×109CFU/kg in drinking water), resveratrol-treated diabetic (10 mg/kg, oral gavage), as well as probiotics and resveratrol-treated diabetic. The treatment procedures lasted for four weeks, and a Shuttle Box test was then performed to evaluate memory and learning. At the end of the study, animals were sacrificed, and the hippocampus was removed to perform biochemical studies. Results: The levels of malondialdehyde and total oxidative status significantly decreased in the diabetic group treated with combined resveratrol and probiotics (P<0.05). Furthermore, the levels of superoxide dismutase, catalase, and glutathione peroxidase significantly increased in the hippocampus of the diabetic group treated with combined resveratrol and probiotics (P<0.05). According to the results, the combined therapy improved memory and learning (P<0.05). In addition, the level of GLP-1 increased in the treatment groups (P<0.05). Conclusion: Treatment with resveratrol and probiotics significantly normalized pyramidal cell densities in the hippocampus of diabetic rats. This combination also reduced oxidative stress and activated the gut-brain axis in diabetic animals.

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Time-dependent impairments in learning and memory in Streptozotocin-induced hyperglycemic rats

Cited by 21 publications

References 63 publications

Comparison of the Protective Effects of Ginsenosides Rb1 and Rg1 on Improving Cognitive Deficits in SAMP8 Mice Based on Anti-Neuroinflammation Mechanism

Comparison of the Protective Effects of Ginsenosides Rb1 and Rg1 on Improving Cognitive Deficits in SAMP8 Mice Based on Anti-Neuroinflammation Mechanism

Jiawei Shengmai San herbal formula ameliorates diabetic associate cognitive decline by modulatingAKTandCREBin rats

Memory and Learning Improvement by Resveratrol and Probiotics via the ‎Gut-Brain Axis and Antioxidant Activity in Diabetic Rats

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