Time-dependent toxicity in the long-term inhibition assay with Vibrio fischeri

Froehner, K.; Meyer, Wiebke; Grimme, L. Horst

doi:10.1016/s0045-6535(01)00209-0

Cited by 44 publications

(14 citation statements)

References 13 publications

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“…Vibrio fischeri bioluminescence method is useful for screening of samples for antibacterial activity, but has some disadvantages. When performed with short incubation time of 30 minutes as published by BERDEN ZRIMEC et al (2004), this method is appropriate only for screening fast acting antimicrobial compounds (FROEHNER et al 2002). Due to the short incubation time, chemicals interfering with biosynthetic pathways show only minor toxicities to Vibrio fischeri (BACKHAUS and GRIMME 1999).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of antibacterial activity of extracts of five species of wood‐colonizing fungi

Janeš

Umek

Kreft

2006

J. Basic Microbiol.

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Screening new organisms for antibacterial activity and searching for new antibacterial drugs is important due to the constant generation of new antibiotic-resistant strains of pathogenic bacteria. An E. coli broth microdilution test was used to evaluate the results of the Vibrio fischeri bioluminescence test in five of the most antibacterially active species of wood-colonizing fungi. Serpula lacrymans was found to be a potential source of thermostable antibiotic(s) and the Vibrio fischeri bioluminescence test was confirmed to be a useful method for screening for antibacterial activity.

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Section: Resultsmentioning

confidence: 99%

Evaluation of antibacterial activity of extracts of five species of wood‐colonizing fungi

Janeš

Umek

Kreft

2006

J. Basic Microbiol.

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“…The photocyte bioassay involves eukaryotic cells that do not replicate and thus have limited adaptability to metal exposure. Similarly, the Microtox bioassay measures acute toxicity originating from a physicochemical change because the bacteria have a slow generation time (Ͼ 2 h) that does not allow adaptation to changes in the surrounding environment over the time of the bioassay (Ross, 1993;Bulich and Bailey, 1995;Froehner et al, 2002). The P. phosphoreum bioassay belongs to the noncatalytic group; the bacteria replicate and possibly adapt to changes in the surrounding environment by changes in gene and/or protein expression (Thomulka et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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ABSTRACT:Metal toxicity is a function of the biology of the target organism and the chemical speciation of the metal. The toxicity of 11 metals was assessed with three cell-based bioassays based on marine organisms: the bacterium Photobacterium phosphoreum of the Microtoxா bioassay, an environmental strain of P. phosphoreum, and photocytes isolated from the brittlestar Ophiopsila californica. Metal speciation was calculated for three commonly used media: NaCl-based Microtoxா bioassay medium, artificial seawater glycerol, and artificial seawater. Decreased bioluminescence was considered a proxy for cell toxicity. In all three assays the elements Cd and Hg exhibited similar speciation as well as similar toxicity profiles. The element Cu was toxic in all three assays despite different metal speciation for the P. phosphoreum bioassay. The element Ag was toxic to both bacterial strains but not to photocytes despite a similar chemical speciation for all three assays. In general, the Microtoxா bioassay was sensitive to all metals (except Pb), whereas the photocytes were the least sensitive to the metals. The heightened response of the Microtoxா bioassay probably resulted from a combination of the limited complexing power of the medium and the greater sensitivity of the bacterial strain.

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“…This test was performed according to the methods and quantities used by Krifaton et al (2013). For data analysis, bioluminescence was determined with an inverse formula of Froehner et al (2002) using bioluminescence intensification, instead of inhibition, as follows:…”

Section: Blyes For Analyzing the Biological Effect Of Zon Residuementioning

confidence: 99%

Biodegradation of aflatoxin-B1 and zearalenone by Streptomyces sp. collection

Harkai

Szabó

Cserháti

et al. 2016

International Biodeterioration & Biodegradation

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a b s t r a c tAflatoxin B1 (AFB1) and zearalenone (ZON) are hazardous mycotoxins. AFB1 has cytotoxic, mutagenic and carcinogenic effects, whereas ZON can disrupt the endocrine system. Biodegradation by microbes is an effective method to eliminate these hazardous toxins. The aim of this work was to screen AFB1 and ZON biodegrading potential of one hundred and twenty-four Streptomyces strains deposited in the Actinomycetes strain collection of the Department of Environmental Safety and Ecotoxicology. Two different biotests were used for screening purposes: SOS-Chromotest was used to monitor genotoxicity and select microorganisms with the best AFB1 degrading potential. Estrogenic effect of ZON was measured with a yeast based bioluminescent test including human estrogen receptors Bioluminescent Yeast Estrogen System (BLYES). Biodegradation experiments were conducted with 1 mg l À1 AFB1 and 1 mg l À1 ZON concentration. On the base of the results, ten strains were selected for biodegradation experiments and Enzyme-linked Immunosorbent Assay tests (ELISA). The results of these tests Streptomyces cacaoi subsp. asoensis (K234) strain degraded AFB1 over 88 per cent and totally eliminated genotoxicity. Two strains of Streptomyces rimosus (K145, K189) degraded almost total amount of ZON and estrogenicity was not detected besides that.

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Time-dependent toxicity in the long-term inhibition assay with Vibrio fischeri

Cited by 44 publications

References 13 publications

Evaluation of antibacterial activity of extracts of five species of wood‐colonizing fungi

Evaluation of antibacterial activity of extracts of five species of wood‐colonizing fungi

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Biodegradation of aflatoxin-B1 and zearalenone by Streptomyces sp. collection

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